Die Identifizierung der Chromosomen im Karyotyp der Rattenleber

Gläss, Eberhard

doi:10.1007/bf00329746

Cited by 30 publications

(4 citation statements)

References 17 publications

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“…Although not stated, this model already suggests the organization of chromosomes in two haploid sets as indicated by us [ 7 ]. The last model also predicts the presence of a certain architecture of CTs in both interphase and metaphase in the way of a haploid grouping, which beyond human samples [ 7 – 13 ] was also found in plant louse cells [ 17 ], rat liver cells [ 14 , 15 ] and in hexaploid vole cells [ 16 ]. …”

Section: Discussionmentioning

confidence: 98%

“…In other words, the parental haploid chromosome sets of diploid cells are well arranged within the nucleus and also within the metaphase stage, when DNA is most condensed and appears as microscopically visible chromosomes. Previously Gläss [ 14 , 15 ] observed the segregation of parental haploid chromosome sets in regenerating liver cells of rats; also Pera [ 16 ] presented a hexaploid metaphase spread in vole cells, where the six sets of chromosomes were apparently lying within distinct haploid domains. Additionally, in insects and plants there are clear examples of separated parental genomes in the nuclei [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Chromosomes in a genome-wise order: evidence for metaphase architecture

et al. 2016

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BackgroundOne fundamental finding of the last decade is that, besides the primary DNA sequence information there are several epigenetic “information-layers” like DNA-and histone modifications, chromatin packaging and, last but not least, the position of genes in the nucleus.ResultsWe postulate that the functional genomic architecture is not restricted to the interphase of the cell cycle but can also be observed in the metaphase stage, when chromosomes are most condensed and microscopically visible. If so, it offers the unique opportunity to directly analyze the functional aspects of genomic architecture in different cells, species and diseases. Another aspect not directly accessible by molecular techniques is the genome merged from two different haploid parental genomes represented by the homologous chromosome sets. Our results show that there is not only a well-known and defined nuclear architecture in interphase but also in metaphase leading to a bilateral organization of the two haploid sets of chromosomes. Moreover, evidence is provided for the parental origin of the haploid grouping.ConclusionsFrom our findings we postulate an additional epigenetic information layer within the genome including the organization of homologous chromosomes and their parental origin which may now substantially change the landscape of genetics.Electronic supplementary materialThe online version of this article (doi:10.1186/s13039-016-0243-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Chromosomes in a genome-wise order: evidence for metaphase architecture

et al. 2016

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“…An ability of adult cells to isolate haploid genomes or even sort out maternal and paternal chromosomes was also deduced from work on rat liver. Diploid metaphase spreads were described to be separable by a single line in two haploid sets of chromosomes while other spreads had only a haploid set of chromosomes ("Genomsonderung";Gläss 1956Gläss , 1957. The latter was also reported for fibroblasts of the mouse Microtus agrestis (Pera and Rainer 1973).…”

Section: Introductionmentioning

confidence: 86%

Parental genomes mix in mule and human cell nuclei

et al. 2009

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Whether chromosome sets inherited from father and mother occupy separate spaces in the cell nucleus is a question first asked over 110 years ago. Recently, the nuclear organization of the genome has come increasingly into focus as an important level of epigenetic regulation. In this context, it is indispensable to know whether or not parental genomes are spatially separated. Genome separation had been demonstrated for plant hybrids and for the early mammalian embryo. Conclusive studies for somatic mammalian cell nuclei are lacking because homologous chromosomes from the two parents cannot be distinguished within a species. We circumvented this problem by investigating the three-dimensional distribution of chromosomes in mule lymphocytes and fibroblasts. Genomic DNA of horse and donkey was used as probes in fluorescence in situ hybridization under conditions where only tandem repetitive sequences were detected. We thus could determine the distribution of maternal and paternal chromosome sets in structurally preserved interphase nuclei for the first time. In addition, we investigated the distribution of several pairs of chromosomes in human bilobed granulocytes. Qualitative and quantitative image evaluation did not reveal any evidence for the separation of parental genomes. On the contrary, we observed mixing of maternal and paternal chromosome sets.

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“…• analyzing ultrathin sections and applying differential in situ suppression hybridization of the two parental sets of chromosomes in monocot plants [40,41] , • in male scale insects (mealy bugs) with paternal chromosomes heterochromatic and condensed all through the cell cycle [42,43] , • in rat liver cells using oxyquinoline banding techniques [44,45] , • in preimplantation embryos of mice following impregnation by spermatozoa completely labeled with bromodeoxyuridine [46,47] , and • in dicot hybrid plants using three-dimensional (3-D)…”

Section: Summary: Intranuclear Order Of Bilateral Symmetry Supports Cmentioning

confidence: 99%

Nuclear Segmentation, Condensation and Bilateral Symmetry in Polymorphonuclear Leukocytes Reflect Genomic Order and Favor Immunologic Function

Chaudhuri¹,

Walther²

2012

Acta Haematol

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Segmentation, condensation and bilateral symmetry of the nuclei of polymorphonuclear leukocytes seem related to their function. Segmentation of the nuclei into two or more lobes and their condensation facilitate their passage (diapedesis) through the endothelial layer of blood vessels to the extravasal space and subsequent locomotion through the interstitial compartment of different tissues. Bilateral symmetry of these nuclei along with their association to the cytoskeletal fibers contribute to their efficiency in locomotion by alignment of the axis of nuclear symmetry to the axis of cellular polarity, which orients towards the direction of locomotion in response to cytokines and other stimuli. Observations of the cytogenetic facets of intranuclear order support these assumptions.

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Die Identifizierung der Chromosomen im Karyotyp der Rattenleber

Cited by 30 publications

References 17 publications

Chromosomes in a genome-wise order: evidence for metaphase architecture

Chromosomes in a genome-wise order: evidence for metaphase architecture

Parental genomes mix in mule and human cell nuclei

Nuclear Segmentation, Condensation and Bilateral Symmetry in Polymorphonuclear Leukocytes Reflect Genomic Order and Favor Immunologic Function

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