Die klinisch-diagnostische Bedeutung lokalisationsspezifischer Enzyme im Harn

Mackenrodt, G.; Zegelman, M.

doi:10.1007/bf01781055

Cited by 5 publications

(1 citation statement)

References 4 publications

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“…Another approach to characterize tubular disease is the determination of tubular epithelial enzymes such as alkaline phosphatase, gamma-glutamyltransferase, alanine-amino-peptidase or maltase in the urine (Burchardt et al 1979;Mondorf et al 1976;Kochmann et al 1980). However, since these and other tubular enzymatic activities are also present in the plasma their excretion might be elevated in glomerular disease as well unless specific immunological methods are applied (Scherberich and Mondorf 1979).…”

Section: Characterization Of Urinary Proteinsmentioning

confidence: 99%

Urinary protein excretion in interstitial and tubular kidney disease as characterized by gradient electrophoresis

et al. 1983

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Summary.In order to estimate the degree of tubular lesions and to detect a possible glomerular involvement, proteinuria was studied in tubular and/ or interstitial nephropathies by determination of total protein and beta-2 microglobulin and by micro-gradientgel electrophoresis. The excretion of albumin, low molecular weight proteins (LMW) and high molecular weight (HMW) proteins was measured in addition. Other proximal tubulus functions such as glucose and phosphate reabsorption were also measured. The clinical entities studied were: chronic postinfectious nephritis, patients with nephropathy following analgesic abuse, tubular glucosuria in pregnancy and cis-platinum treated patients suffering from testicular tumor. The results were interpreted basing on the "selectivity" or urinary protein patterns, which means the relation between LMW and HMW proteins. Micro-gradientgel electrophoresis proved to be a fast and sensitive tool for quantitative as well as qualitative analysis of albumin, LMW and HMW proteins.All patients with advanced tubulo-interstitial disease excreted increased amounts of LMW proteins. The excretion of beta-2 microglobulin was also high and correlated with the LMW protein excretion. The albumin and HMW protein excretion showed no relation to LMW proteins. The disproportionate increase of LMW protein excretion on the one hand and albumin and HMW protein excretion on the other suggests different reabsorption mechanisms. The tubular patterns originate from a more easily disturbed reabsorption of LMW proteins and/or from a normally more active reabsorption of LMW proteins. Except in renal glucosuria phosphate and glucose excretion were not increased. This means that the capacity Offprint requests to: Dr. rer. nat. J. Alt (address see page 648) for the reabsorption of glucose and phosphate copes with a normal filtered load even in patients with highly increased proteinuria of a tubular type.

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Section: Characterization Of Urinary Proteinsmentioning

confidence: 99%

Urinary protein excretion in interstitial and tubular kidney disease as characterized by gradient electrophoresis

et al. 1983

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Quantification of nephrotoxicity in rabbits by automated morphometry of alkaline phosphatase stained kidney sections

Wachsmuth

1981

Histochemistry

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Male and female rabbits were injected intravenously with a single dose of either cefroxadine or cefsulodin or cephaloridine. Quantitative determinations of the activity of two brush border membrane enzymes, aminopeptidase and alkaline phosphatase, were made in homogenates of cortical kidney tissue, in the urine and morphometrically in proximal tubules of cryostat sections. Morphometry was done by classification and enumeration of proximal tubule sections with the same level of enzyme reaction product using a microscopic television analysis system. By comparison with the control values, no changes were detectable 24 h after the injection of up to 1.2 g cefroxadine or cefsulodin per kg body weight. By contrast, after 300 mg/kg cephaloridine, the concentrations of the two enzymes were decreased in a large number of proximal tubules, i.e. the brush border membranes, and concomitantly cell degeneration and necrosis took place. Alkaline phosphatase activity in sections and tissue homogenates was reduced to a greater extent than aminopeptidase activity. A corresponding, significant increase in enzymic activity in the urine was only demonstrable in respect of aminopeptidase. The classification of proximal tubules in sections by television analysis on the basis of alkaline phosphatase reaction product concentration appears to be a reliable measure for detecting and quantifying toxic effects on proximal tubules of kidney.

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Effect of aminoglycosides on proximal tubular membranes of the human kidney

Mondorf

Breier

Hendus

et al. 1978

Eur J Clin Pharmacol

104

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The effect of the aminoglycosides amikacin, gentamicin, netilmicin sisomicin and tobramycin on the proximal tubule of the human kidney was investigated in 78 healthy subjects. Fifteen adults were each given gentamicin, sisomicin or tobramycin 3 mg/kg bodyweight, 10 subjects received netilmicin 3 mg/kg or amikacin 15 mg/kg additionally seven subjects amikacin 10 mg and six subjects netilmicin 6 mg on three consecutive days. The principal enzyme of the brush border membrane of the proximal tubule, alanine aminopeptidase (AAP), was determined enzymatically and immunologically in 24-hour urines. The effects of the various aminoglycosides on the membranes were different. Less of membrane AAP was greatest after amikacin and was least after tobramycin. There was no difference between gentamicin, netilmicin, and sisomicin, which had an effect intermediate between the other two compounds. The elimination of AAP occurred at intervals which might possibly have been caused by impairment of cell cycles.

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Die klinisch-diagnostische Bedeutung lokalisationsspezifischer Enzyme im Harn

Cited by 5 publications

References 4 publications

Urinary protein excretion in interstitial and tubular kidney disease as characterized by gradient electrophoresis

Urinary protein excretion in interstitial and tubular kidney disease as characterized by gradient electrophoresis

Quantification of nephrotoxicity in rabbits by automated morphometry of alkaline phosphatase stained kidney sections

Effect of aminoglycosides on proximal tubular membranes of the human kidney

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