Die Lipotropine

Schwandt, P.

doi:10.1007/bf01614390

Cited by 9 publications

(3 citation statements)

References 82 publications

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“…There is considerable evidence that the pituitary gland occupies a central position among the different organs producing lipolytic substances (Astwood, 1965;Chretien, Lis, Bertagna & Gilardeau, 1973); numerous lipolytically active peptides, mostly isolated from the pituitary gland, have been described (Schwandt, 1974). Although extensive work on the fat mobilizing properties of the pituitary peptides has been carried out, a question remains unsettled; do they play a physiological role in lipid mobilization?…”

Section: Introductionmentioning

confidence: 98%

An Extra-Adrenal Action of Adrenocorticotrophin: Physiological Induction of Lipolysis by Secretion of Adrenocorticotrophin in Obese Rabbits

Lafontan¹,

Agid²

1979

Journal of Endocrinology

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The effect of minor stressful stimuli (saline infusions or venepunctures) on lipid mobilization (estimated by plasma free fatty acids (FFA) and glycerol levels) was studied in two strains of conscious rabbits of various ages. Infusions of 0.9% (w/v) NaCl solution induced a prompt increase of FFA levels in naturally obese rabbits while this effect was absent in younger lighter animals. Administration of propranolol (a beta-adrenoceptor blocking drug) beforehand did not prevent lipid mobilization. Adipose tissue in vitro was completely unresponsive to the lipid mobilizing action of adrenaline. The initial output of ACTH induced by stressful stimuli was quickly followed by the increase in the plasma concentration of FFA. A highly significant correlation (r = 0.84 or 0.85; P less than 0.01) existed between plasma ACTH and FFA levels 30 and 60 min after the start of stress. Dexamethasone completely prevented lipid mobilization while adipose tissue in vivo and in vitro responded strongly to ACTH. Consequently the lack of response of plasma FFA concentrations in corticosteroid-treated rabbits seemed to be linked to the failure of the pituitary gland to mobilize ACTH rather than to the unresponsiveness of adipose tissue to the pituitary hormone. Our data indicate that in the rabbit, under conditions of stress, ACTH is of greater physiological importance than catecholamines in the control of lipolysis.

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Section: Introductionmentioning

confidence: 98%

An Extra-Adrenal Action of Adrenocorticotrophin: Physiological Induction of Lipolysis by Secretion of Adrenocorticotrophin in Obese Rabbits

Lafontan¹,

Agid²

1979

Journal of Endocrinology

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“…Besides a number of hormones released in times of stress, lipolysis seems to be stimulated by a specifie lipolytie hormone, found in the pituitary (Schwandt 1974). In our laboratory two peptides P-LF 11 C and P-LF 11 0 were isolated from porcine pituitary glands whieh displayed higher lipolytic aetivity than cortieotropin which is known to be the most potent lipolytie hormone of the pituitary (Schleyer, Voigt and Pfelffer 1976a).…”

Section: Introductionmentioning

confidence: 98%

Studies on the Pituitary "Fettstoffwechselhormon" - VIII. Radioimmunoassay of the Lipolytic Factor P-LF II D

Etzrodt¹,

Schleyer²,

Pfeiffer³

1978

Horm Metab Res

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A radioimmunoassay of the lipolytic peptide P-LF II D from porcine pituitaries is described. The assay is performed with 125-iodine labeled P-LF II D and with antisera either from guinea pigs or from rabbits. Bound antigen is separated from the free by double antibody technique. No cross reaction is observed with gamma lipotropin, peptide B, secretin, glucagon, isoproterenol. Due to contamination P-LF II C, beta lipotropin and human growth hormone displace the tracer when added at large doses. Complete cross reaction is observed between porcine 1-39 ACTH and P-LF II D. Specificity of this reaction is demonstrated by the increase of cross reaction, when ACTH fragments of increasing length of the polypeptide chain are used (1-23 ACTH, 1-24 ACTH and 1-28 ACTH).

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“…The isolation of lipolytic peptides from pituitary glands has been reported (Schwandt 1974) for various species. From pig pituitary glands we have iso¬ lated peptide B; its lipolytical and other metabolic effects have been described elsewhere (Schwandt 1973;Weisweiler 8c Schwandt 1975).…”

mentioning

confidence: 99%

Radioimmunoassay for Porcine Lipotrophic Peptide B

Neureuther¹,

Schwandt²,

Otto³

1977

Acta Endocrinologica

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A sensitive radioimmunoassay for the lipolytic peptide B from pig pituitary glands has been developed. Antisera were raised in rabbits, which allowed a final dilution of 1:6000. Free and antibody bound [125I]peptide B could be separated by double antibody, by dextran-coated charcoal and by polyethyleneglycol. The smallest detectable amount was 0.5 ng/ml. Peptide B could be measured irregularly in porcine sera; there was crossreacting material in an extract of a total human pituitary gland, but not in human sera. The assay seems specific except for a strong cross-reaction with porcine neurophysin. There are indications that peptide B consists of neurophysin and a small lipolytically active peptide which remains to be further characterized.The isolation of lipolytic peptides from pituitary glands has been reported (Schwandt 1974) for various species. From pig pituitary glands we have iso¬ lated peptide B; its lipolytical and other metabolic effects have been described elsewhere (Schwandt 1973; Weisweiler 8c Schwandt 1975). The acidic (Ip. 4.39) peptide B from pig pituitary glands, which has a molecular weight of about 11 000, is homogenous in column chromatography and analytical acrylamidgelelectrophoresis, has only one N-terminal aminoacid (Ruschewski et al. 1973) and contains two tyrosine residues. For further studies on the physiological relevance of lipotrophic hormones and the regulation mechanisms of lipolysis we tried to set up a radioimmunoassay for the lipolytic peptide B.

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Die Lipotropine

Cited by 9 publications

References 82 publications

An Extra-Adrenal Action of Adrenocorticotrophin: Physiological Induction of Lipolysis by Secretion of Adrenocorticotrophin in Obese Rabbits

An Extra-Adrenal Action of Adrenocorticotrophin: Physiological Induction of Lipolysis by Secretion of Adrenocorticotrophin in Obese Rabbits

Studies on the Pituitary "Fettstoffwechselhormon" - VIII. Radioimmunoassay of the Lipolytic Factor P-LF II D

Radioimmunoassay for Porcine Lipotrophic Peptide B

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