Die PAS-AO-Methode, eine Spezialf�rbung f�r Coccidien im Gewebe

Wagner, W. H.; Foerster, O.

doi:10.1007/bf00348666

Cited by 32 publications

(6 citation statements)

References 10 publications

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“…The horseshoe-shaped distribution of fluorescent material in some second generation merozoites of E. tenella is similar to that reported for the electron-dense material seen in electron micrographs of these stages (McLaren, 1969;Senaud & Cerna, 1969). In view of a similar fluorescence distribution in macrogametes, it is plausible that these second generation merozoites in fact represent macrogamontoblasts (Overdulve, 19786), which means that second generation merozoites are already sexually differentiated before entering a new host cell, as claimed by Klimes, Rootes & Tanielian (1972) and Wagner & Foerster (1964).…”

Section: Comparison With Electron Microscopic Observationssupporting

confidence: 76%

Cytochemical studies on nuclear DNA of four eucoccidian parasites,Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruziandPlasmodium berghei

1984

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Feulgen-pararosaniline (SO2) staining was performed on stages in the life-cycle of Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruzi and Plasmodium berghei. The fluorescence emission of the stained DNA in nuclei of these stages was examined and compared with absorption microscopy measurements at 560 nm (green light) of the same specimens. Accurate identification of single cells, and especially discrimination between young schizonts and young gamonts was difficult after Feulgen staining. To overcome this problem preparations were first stained with Giemsa and the cells of interest precisely located with the aid of an England finder. The same preparations were then hydrolysed and stained with Feulgen-pararosaniline (SO2), after which the previously identified cells were investigated. The DNA distribution after Feulgen staining corresponded with the shape of nuclei after Giemsa staining. DNA was present in all investigated stages of the four parasites, including macrogamonts of I. (T.) gondii and E. tenella and peripheral blood gamonts of P. berghei. Macrogamonts could be recognized, even at a stage at which they can hardly be differentiated from young schizonts in Giemsa-stained preparations, by their typical distribution pattern of Feulgen fluorescence. Feulgen fluorescence was more granular and confined to the peripheral region of the nucleus, leaving a distinct nucleolus unstained. The horseshoe-shaped nuclei typical of macrogamonts could also be observed in some second generation merozoites of E. tenella, indicating that these merozoites are already sexually differentiated. The relationship between the present cytochemical observations and the ultrastructure of the four investigated parasites is discussed.

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Section: Comparison With Electron Microscopic Observationssupporting

confidence: 76%

Cytochemical studies on nuclear DNA of four eucoccidian parasites,Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruziandPlasmodium berghei

1984

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“…Segregation of wall materials in a pattern suggesting a zonal mode of growth was first demonstrated independently by Cole and Hahn (3) and by May (5), and later for D . pneumoniae by Wagner (11) . These studies employed immunofluorescence techniques .…”

Section: Discussionmentioning

confidence: 99%

“…Studies of bacterial wall growth, employing fluorescent antibodies to label specific areas of the growing walls, have suggested that in Gram-positive bacteria wall growth occurs by the intercalation of newly synthesized wall materials into discrete, specific equatorial zones of the preexistent wall (2,3,5,11) .…”

Section: Introduction Materials and Methodsmentioning

confidence: 99%

Radioautographic Evidence for Equatorial Wall Growth in a Gram-Positive Bacterium

Briles

Tomasz

1970

The Journal of Cell Biology

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“…For determination as to whether mAb 35B9 recognized amylopectin itself the sugar was degraded by exposure of methanol-®xed merozoites to a-amylase (1 U/ml, 30 min) and, subsequently, to a-glucosidase (1 U/ml, 30 min). The degradation of carbohydrates was obviously complete as they could no longer be demonstrated in the parasites by PAS-AO (periodic acid-SchiQuiline/orange) staining (Wagner and Foerster 1964). Nevertheless, the binding of mAb 35B9 was unaected as determined by IFAT (data not shown).…”

mentioning

confidence: 91%

A monoclonal antibody reacts species-specifically with amylopectin granules of Eimeria bovis merozoites

Heise

Peters

Zahner

1999

Parasitology Research

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An IgG1 monoclonal antibody (mAb 35B9) developed against first-generation merozoites of Eimeria bovis was shown by immunoelectron microscopy to react selectively with antigens localized in amylopectin granules. Amylopectin does not contribute to the epitope, as enzymatic degradation of carbohydrates in the parasite did not alter the binding pattern of mAb 35B9. When tested by immunoblotting, despite its organelle specificity the mAb recognized a variety of E. bovis merozoite I components with predominant molecules of 135 and 200 kDa. The epitope was not affected by treatment with endoglycosidase H; thus, N-linked sugar residues should not be involved in it. Alkaline cleavage (beta-elimination), however, destroyed the epitope; thus, the involvement of O-linked carbohydrates cannot be excluded. Treatment of E. bovis merozoite extract with phospholipase C changed the binding pattern of mAb 35B9 in a way that suggests the presence of phosphorylcholine molecules on several antigens recognized by the mAb, albeit not belonging to the epitope but rather masking it. The epitope was not found in free sporozoites of E. bovis or young intracellular parasites up to day 4 after invasion of cells in vitro, whereas 5-day-old trophozoites were found to contain it. It seems to be species-specific, as it could not be shown in sporozoites or merozoites of E. tenella or in stages of several other Coccidia.

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Die PAS-AO-Methode, eine Spezialf�rbung f�r Coccidien im Gewebe

Cited by 32 publications

References 10 publications

Cytochemical studies on nuclear DNA of four eucoccidian parasites,Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruziandPlasmodium berghei

Cytochemical studies on nuclear DNA of four eucoccidian parasites,Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruziandPlasmodium berghei

Radioautographic Evidence for Equatorial Wall Growth in a Gram-Positive Bacterium

A monoclonal antibody reacts species-specifically with amylopectin granules of Eimeria bovis merozoites

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