Die Peroxydasereaktionen und ihre praktische Bedeutung

Undritz, E

doi:10.1007/978-3-642-94866-4_15

Cited by 14 publications

(3 citation statements)

References 6 publications

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“…The MPO reaction permits a definite identification of various types of leuko cytes, as well as identification of different developmental stages of myelogenous cells, in both healthy and sick subjects. MPO reactions carried out in bone marrow and peripheral hlood smears from patients with hematological disorders showed no deviation from normal [9,11,12,18,19]. Hence myeloblastic leukemia can be differentiated from other types of leukemia.…”

Section: [9]-mentioning

confidence: 98%

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On the Myeloperoxidase Reaction of Leukocytes

Jd¹

1968

Enzymol Biol Clin

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Summary. There exist several cytochemical reactions known as ''peroxidase'' reactions or ''oxidase reactions'' employed to detect myeloperoxidase (MPO) activity in leukocytes. These reactions like those of Armitage, Graham, Graham-Knoll, Sato-Sekiya, Washburn, Winkler and other authors, very often do not give positive results when dealing with the most immature forms of myelogenous cells, namely myeloblasts. Qualitative and quantitative tests of the reagents used in these reactions were carried out. The demonstration and localization of the enzyme activity by means of these reactions depend primarily on the correct choice of the concentration of the reagents used, as well as on the concentration of myeloperoxidase contained in granulocytic cells. Benzidine base and hydrogen peroxide have been employed in quantities necessary to develop the cytochemical myeloperoxidase reaction in optimal biochemical conditions. The compound developed by these cytochemical methods produces a greenish-yellow colour in the cytoplasm of myelogenous cells. Two new methods are reported: one for bone marrow smears and the other for peripheral blood smears. With the former positive results have been obtained in myeloblasts, which promises to be helpful in the differential diagnosis of myeloblastic leukemia from other types of leukemia.

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Section: [9]-mentioning

confidence: 98%

“…This seems to be the most important clinical application of the MPO reaction. It can be of great help considering the fact that a precise differential diagnosis indicates the most suitable therapy [11,12,18,19].…”

Section: [9]-mentioning

confidence: 99%

On the Myeloperoxidase Reaction of Leukocytes

Jd¹

1968

Enzymol Biol Clin

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“…

One of the most usual methods for the demonstration of peroxidase in blood and bone marrow smears is that of G raham and K noll, several modifications of which were developed by U ndritz [5,6]. However, very weak activities are not readily demonstrable by these methods.

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confidence: 99%