Die Typisierung von Streptococcus faecalis- und Streptococcus faecium-Stämmen mit Bakteriophagen

Biotyping, serotyping and phage typing of Streptococcus faecalis isolated from dental plaque in the human mouth

“…Although clinical isolates of S. faecalis and S. faecium from various sources have been serotyped or phage-typed (Sharpe and Shattock, 1952;Plekeai, 1967Plekeai, , 1982Brandis et al, 1985), oral isolates of enterococci have commonly only been speciated or described as varieties (Williams et al, 1950;Winkler and Van Amerongen, 1959;Gold et al, 1975;Campbell et al, 1983). Thus, although oral carriage of enterococci is by no means a novel finding, detailed data on the biotypes, antibiotic susceptibility phenotypes, serotypes and phage types of such strains have not been available previously.…”

“…All possessed the same biotype, phage type and serotype profile I/II/l. Because the phage subtype 11 was relatively rare among German clinical isolates (Brandis et al, 1985), several of these were investigated. Excluding haemolytic properties of strains which have been shown to be determined by transmissible plasmids (Dunny and Clewell, 1975;Jacob et al, 1975), eight of these clinical isolates differed in only two biochemical characters from the oral biotype-I isolates; six of these belonged to the Sharpe and Shattock serotype 1.…”

“…S. faecalis of phage subtype V6b were the most common amongst clinical isolates in a German survey (Brandis et al, 1985), as well as amongst oral isolates in Eire. Although most of the latter were of biotype 111, the occurrence of four biotype I1 isolates and the finding that strain M440 was biotype I indicates that biotyping may be useful for discrimination within this phage type for epidemiological purposes.…”

“…faecalis strains and of the one S. durans isolate was performed according to the scheme of PlekeaS (1979) as modified by Brandis et al (1985), with 17 phages for the former and seven for the latter. The…”

“…Since a survey of 441 German strains of S. faecalis from different clinical sources had revealed only 19 strains (4.3%) of phage type I1 (Brandis et al, 1985), a number of these were examined for bio type, phage type, serotype and tetracycline susceptibility (tables IV and V).…”