Diel and Circadian Patterns of Locomotor Activity in the Adults of Diamondback Moth (Plutella xylostella)

Wang, Danfeng; Guang, Yang; Chen, Wenfeng

doi:10.3390/insects12080727

Cited by 13 publications

(14 citation statements)

References 38 publications

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“…Consistent with our previous study (Wang et al. , 2021), eduction graphs of average activities over several days revealed that the wild‐type moths showed nocturnal activity under the LD cycle, with activity peaking very early after lights off and quickly declining after lights on (Fig. 3B).…”

Section: Resultssupporting

confidence: 91%

“…To evaluate whether P. xylostella PER is involved in the control of circadian rhythmicity of locomotor activity, the locomotor activities of wild‐type and mutant adults were monitored using a previously described system (Wang et al. , 2021). From the actogram plots, a graphical representation of phases of activity and rest over the course of a day under LD and DD conditions, we found that the wild‐type moths had more obvious locomotor rhythmicity than PxPer ‐KO (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…A LAM16 activity monitor system we previously established for measuring locomotor activities of individual P. xylostella was used to monitor the circadian rhythms of adult wild‐type and per ‐knockout moths (Wang et al. , 2021). Briefly, male P. xylostella were placed into the tubes with a supply of food and activity was monitored using the LAM16 activity monitors.…”

Section: Methodsmentioning

confidence: 99%

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CRISPR/Cas9‐mediated knockout of period reveals its function in the circadian rhythms of the diamondback moth Plutella xylostella

et al. 2022

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Circadian clocks control the rhythmicity of many behaviors and physiological features of insects. To study the circadian clock of the moth Plutella xylostella, we employed CRISPR/Cas9-mediated genome editing to investigate the effect of loss of the clock gene period on the circadian rhythms. P. xylostella harbors a single copy of period. Phylogenetic analysis showed that P. xylostella PERIOD is more homologous to mouse PERIOD than the PERIOD proteins from bees, flies, mosquitos, and many other Lepidoptera, such as Danaus plexippus and Bombyx mori. The circadian rhythms in adult locomotor activity were altered in the period knockout strain of P. xylostella under lightdark (LD) and continuous dark (DD) conditions. Under the LD cycle, the wild-type moths displayed nocturnal activity with activity peaking very early after lights off and quickly declining after lights on. In contrast, the period knockout strain had no peak in activity when the lights were turned off and exhibited steady activity throughout the hours of darkness. Interestingly, under DD conditions, our results showed that the locomotor rhythm can be maintained without period gene, but at a lower rhythmicity ratio than wild-type. In addition, knockout of period in P. xylostella changed circadian rhythms patterns related to pupal eclosion, mating, egg-laying, and egg hatching. Mechanistically, loss of PERIOD disrupted the molecular rhythm of period and changed the clock transcription rhythm in the heads of the moths under LD and DD conditions. Together, our study indicates that the PERIOD is required for normal expression of many behavioral rhythms in P. xylostella.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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CRISPR/Cas9‐mediated knockout of period reveals its function in the circadian rhythms of the diamondback moth Plutella xylostella

et al. 2022

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“…, 1990; Wang et al. , 2021). The cry1 mutants showed more activities than G88 in daytime, in other words, the mutant of cry1 caused a certain defect in P .…”

Section: Discussionmentioning

confidence: 99%

Knockout of cryptochrome 1 disturbs the locomotor circadian rhythm and development of Plutella xylostella

Chen

Wang

et al. 2022

Insect Science

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Cryptochrome 1 (CRY1) functions as a light-responsive photoreceptor, which is crucial for circadian rhythms. The identity and function of CRY1 in Plutella xylostella remain unknown. In this study, cry1 was cloned and identified in P. xylostella. Then, a cry1-knockout strain (Cry1-KO) of P. xylostella with a 2-bp deletion was established from the strain Geneva 88 (G88) using the CRISPR/Cas9 technology. No daily temporal oscillation of cry1 was observed in G88 and Cry1-KO, and cry1 mean daily transcription of Cry1-KO was lower than that of G88. Both G88 and Cry1-KO demonstrated rhythmic locomotion under the light/dark condition with Cry1-KO being more active than G88 in the daytime, whereas Cry1-KO completely lost rhythmicity under constant darkness. The developmental period of pre-adult of Cry1-KO was longer than that of G88; the lifespan of the Cry1-KO male adult was shorter than that of G88; the fecundity of Cry1-KO was lower than that of G88; and Cry1-KO showed lower intrinsic rate of increase (r), net reproduction rate (R 0 ), finite increase rate (λ), and longer mean generation time (T) than G88. Our results indicate that cry1 is involved in the regulation of locomotor circadian rhythm and development in P. xylostella, providing a potential target gene for controlling the pest and a basis for further investigation on circadian rhythms in lepidopterans.

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“…A gender difference in the phototaxis behavior was also observed in the P. xylostella LW-opsin mutant [ 17 ]. Moreover, the P. xylostella males exhibited a better locomotor circadian rhythm (induced by light) than the females [ 43 ]. These results suggest that the phototransduction mechanism might be diverse between P. xylostella male and females.…”

Section: Discussionmentioning

confidence: 99%

An LW-Opsin Mutation Changes the Gene Expression of the Phototransduction Pathway: A Cryptochrome1 Mutation Enhances the Phototaxis of Male Plutella xylostella (Lepidoptera: Plutellidae)

Chen

Lin

Qiu

et al. 2023

Insects

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Plutella xylostella is a typical phototactic pest. LW-opsin contributes to the phototaxis of P. xylostella, but the expression changes of other genes in the phototransduction pathway caused by the mutation of LW-opsin remain unknown. In the study, the head transcriptomes of male G88 and LW-opsin mutants were compared. A GO-function annotation showed that DEGs mainly belonged to the categories of molecular functions, biological processes, and cell composition. Additionally, a KEGG-pathway analysis suggested that DEGs were significantly enriched in some classical pathways, such as the phototransduction-fly and vitamin digestion and absorption pathways. The mRNA expressions of genes in the phototransduction-fly pathway, such as Gq, ninaC, and rdgC were significantly up-regulated, and trp, trpl, inaD, cry1, ninaA and arr1 were significantly down-regulated. The expression trends of nine DEGs in the phototransduction pathway confirmed by a RT-qPCR were consistent with transcriptomic data. In addition, the influence of a cry1 mutation on the phototaxis of P. xylostella was examined, and the results showed that the male cry1 mutant exhibited higher phototactic rates to UV and blue lights than the male G88. Our results indicated that the LW-opsin mutation changed the expression of genes in the phototransduction pathway, and the mutation of cry1 enhanced the phototaxis of a P. xylostella male, providing a basis for further investigation on the phototransduction pathway in P. xylostella.

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Diel and Circadian Patterns of Locomotor Activity in the Adults of Diamondback Moth (Plutella xylostella)

Cited by 13 publications

References 38 publications

CRISPR/Cas9‐mediated knockout of period reveals its function in the circadian rhythms of the diamondback moth Plutella xylostella

CRISPR/Cas9‐mediated knockout of period reveals its function in the circadian rhythms of the diamondback moth Plutella xylostella

Knockout of cryptochrome 1 disturbs the locomotor circadian rhythm and development of Plutella xylostella

An LW-Opsin Mutation Changes the Gene Expression of the Phototransduction Pathway: A Cryptochrome1 Mutation Enhances the Phototaxis of Male Plutella xylostella (Lepidoptera: Plutellidae)

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