Dielectrophoretic platforms for bio-microfluidic systems

Khoshmanesh, Khashayar; Nahavandi, Saeid; Baratchi, Sara; Mitchell, Arnan; Kalantar‐zadeh, Kourosh

doi:10.1016/j.bios.2010.09.022

Cited by 334 publications

(271 citation statements)

References 142 publications

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“…Several different designs of DEP separation devices have been developed, [17][18][19][20] with the inhomogeneity of the electric field generating the DEP force achieved either by the shapes of the electrodes, referred to as classical DEP, 21,22 or by inclusion of insulating structures into the otherwise largely uniform field, referred to as insulator DEP (iDEP) 23,24 and contactless DEP (cDEP). 25,26 Dielectrophoretic separation is based on bioelectrical cell properties and is independent of the cells' genotype.…”

Section: Introductionmentioning

confidence: 99%

Enhanced contactless dielectrophoresis enrichment and isolation platform via cell-scale microstructures

et al. 2016

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We designed a new microfluidic device that uses pillars on the same order as the diameter of a cell (20 lm) to isolate and enrich rare cell samples from background. These cell-scale microstructures improve viability, trapping efficiency, and throughput while reducing pearl chaining. The area where cells trap on each pillar is small, such that only one or two cells trap while fluid flow carries away excess cells. We employed contactless dielectrophoresis in which a thin PDMS membrane separates the cell suspension from the electrodes, improving cell viability for off-chip collection and analysis. We compared viability and trapping efficiency of a highly aggressive Mouse Ovarian Surface Epithelial (MOSE) cell line in this 20 lm pillar device to measurements in an earlier device with the same layout but pillars of 100 lm diameter. We found that MOSE cells in the new device with 20 lm pillars had higher viability at 350 V RMS , 30 kHz, and 1.2 ml/h (control 77%, untrapped 71%, trapped 81%) than in the previous generation device (untrapped 47%, trapped 42%). The new device can trap up to 6 times more cells under the same conditions. Our new device can sort cells with a high flow rate of 2.2 ml/h and throughput of a few million cells per hour while maintaining a viable population of cells for off-chip analysis. By using the device to separate subpopulations of tumor cells while maintaining their viability at large sample sizes, this technology can be used in developing personalized treatments that target the most aggressive cancerous cells. V C 2016 AIP Publishing LLC. [http://dx

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Section: Introductionmentioning

confidence: 99%

Enhanced contactless dielectrophoresis enrichment and isolation platform via cell-scale microstructures

et al. 2016

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“…A particle moves to weaker electric field regions if the particle is less polarizable than the medium, called negative DEP. [28][29][30][31][32][33] To migrate a particle in an electric field using dielectrophoresis, there must be an electric field gradient. In a uniform electric field region, although particles are polarized, no net DEP forces can be induced for moving.…”

Section: High Aspect Ratio Deformable Membranes For 3d Dielectrophorementioning

confidence: 99%

Fabrication of 3D high aspect ratio PDMS microfluidic networks with a hybrid stamp

et al. 2015

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“…The dielectrophoretic force is sensitive to electrode design, based on the previous reports, 44 this study adopted COMSOL MULTIPHYSICS 3.5 (COMSOL, Inc., Burlington) to simulate the gradient of electric field square (E 2 ) and the electric field distribution in the electrode plane. A 2D simulation was performed at quasi-static conditions.…”

Section: B Numerical Simulationmentioning

confidence: 99%

A capillary dielectrophoretic chip for real-time blood cell separation from a drop of whole blood

Liao

Chang²,

Chang

2013

Biomicrofluidics

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This study proposes a capillary dielectrophoretic chip to separate blood cells from a drop of whole blood (approximately 1 ll) sample using negative dielectrophoretic force. The separating efficiency was evaluated by analyzing the image before and after dielectrophoretic force manipulation. Blood samples with various hematocrits (10%-60%) were tested with varied separating voltages and chip designs. In this study, a chip with 50 lm gap design achieved a separation efficiency of approximately 90% within 30 s when the hematocrit was in the range of 10%-50%. Furthermore, glucose concentration was electrochemically measured by separating electrodes following manipulation. The current response increased significantly (8.8-fold) after blood cell separation, which was attributed not only to the blood cell separation but also to sample disturbance by the dielectrophoretic force.

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Dielectrophoretic platforms for bio-microfluidic systems

Cited by 334 publications

References 142 publications

Enhanced contactless dielectrophoresis enrichment and isolation platform via cell-scale microstructures

Enhanced contactless dielectrophoresis enrichment and isolation platform via cell-scale microstructures

Fabrication of 3D high aspect ratio PDMS microfluidic networks with a hybrid stamp

A capillary dielectrophoretic chip for real-time blood cell separation from a drop of whole blood

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