Dielektrophorese als Grundlage für ein neues Verfahren zur präparativen Zelltrennung

Gläser, Roland; Pescheck, C; Krause, G.; Kp, Schmidt; Teuscher, L.

doi:10.1002/jobm.3630190902

Cited by 12 publications

(4 citation statements)

References 8 publications

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“…Close membrane contact is of course one of the prerequisites for fusion. With the field-induced fusion technique close membrane contact is achieved by dielectrophoresis and mutual interaction between cells which have dipoles generated by the field [23,35,37,41,44,62]. The term dielectrophoresis should not be confused with electrophoresis.…”

Section: Dielectrophoresismentioning

confidence: 98%

“…This leads to the formation of "pearl chains" of cells the neutral particle will still move towards the region of highest field intensity; this means that, in contrast to electrophoresis, dielectrophoresis also occurs in an a-c field. As in all polarization processes, the force is proportional to the square of the field strength [23,41,44,54,55,62,63]. Dielectrophoresis (particularly of small cells) usually requires quite divergent fields for a strong effect.…”

Section: Fig 1 (A)mentioning

confidence: 99%

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Electric field-induced cell-to-cell fusion

1982

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Cell-to-cell fusion has great potential in membrane research, membrane reconstitution and genetic mapping [25,34,49]. Furthermore, both somatic hybridization and genetic engineering offer a way of modifying plant cells and, in turn, of improving crops [49,53]. If lymphocyte cells are fused with a permanent cell line so-called hybridoma cells are produced [32] which are capable of producing monoclonal antibodies of predetermined antigenic specificity. These hybridoma cells (antibody-producing hybrids) can be envisaged as important tools in future clinical diagnosis and therapy as well as in the purification and enrichment of compounds of cell-biological and medical interest [18,65, 72]. However, despite many promising results and considerable efforts, fusion still seems to be something of an art rather than a precise science.Phenomenologically, cell-to-cell fusion is achieved presently in vitro by chemicals or inactivated virus [2,30,31,33,34,48,64]. Fusion can usually only be achieved by using membrane-disrupting agents and procedures and/or unphysiological conditions (e.g., high Ca/+ concentrations, high or low pH values, hypotonic conditions, etc.)The field of chemically and virus-induced fusion has been excellently reviewed by several authors over the last years [17,34,45,47,48]. In view of the bewildering array of data on chemically and virusinduced fusion we are obliged to conclude that we are still far from solving the molecular mechanism underlying the fusion process.Any progress in this field and in the development of novel fusion techniques will have to be evaluated by comparison with the inherent limitations of the current fusion procedures, which are listed below:

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Section: Dielectrophoresismentioning

confidence: 98%

Section: Fig 1 (A)mentioning

confidence: 99%

Electric field-induced cell-to-cell fusion

1982

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“…The essential characteristic of DEP is the movement of objects with respect to their suspending medium; thus particles can be concentrated to a focal point by negative DEP or trapped by positive DEP, and different particle types can be moved apart from one another all under appropriate field conditions. These basic manipulations can be used to sort, isolate, and trap particles, cells and microbeads [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30].…”

Section: Theorymentioning

confidence: 99%

Particle separation by dielectrophoresis

2002

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“…Best known among them are the collections of cells at the electrodes (Pohl and Hawk, 1966;Mason and Townsley, 1971) and the alignment of cells in form of "pearl chains" along the lines of the electric field (Teixeira-Pinto et al, 1960;Furedi and Ohad, 1964;Glaser et al, 1979), a procedure which is used in recent times as prerequisite for cell fusion by electric pulses (Scheurich et al, 1980;Zimmermann, 1982). Moreover, orientation of prolonged particles perpendicular or parallel to the field lines in dependence upon the frequency is observed in many systems (Griffin and Stowell, 1960;Teixeira-Pinto et al, 1960;Furedi and Ohad, 1964} or rotation of cells in alternating (Furedi and Ohad, 1964;Mischel and Lamprecht, 1980;Holzapfel et al, 1982) or rotating external fields (Arnold and Zimmermann, 1982;Mischel et al, 1982).…”

Section: Introductionmentioning

confidence: 98%

Dynamic and static cord-structure: A new dielectrophoretic phenomenon

et al. 1983

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During the dielectrophoresis of ceils or other particles it is observed that unusual distributions of the cells appear on the floor of the test chamber. In certain narrow frequency ranges, the distributions are cord-like, with the "cords" running more or less parallel to the electrode faces, but also developing highly branched structures. It is observed that the cells within the cords form "pearl-chains" along the field lines and across the cords. Within the cords, the cells move actively in spiralized paths. This the dynamic cord-structure is considered to be due to the combined action of negative dieleetrophoresis, Brownian motion, and gravity. Outside the conditions requisite for dynamic cord formation, the cells are seen to assume conformations reflective of simple negative dielectrophoresis which merely causes them to amass into regions of low field strength. The theory for the effects is presented, together with experimental evidence for the kinetic behavior of the dynamic cord effect.

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Dielektrophorese als Grundlage für ein neues Verfahren zur präparativen Zelltrennung

Cited by 12 publications

References 8 publications

Electric field-induced cell-to-cell fusion

Electric field-induced cell-to-cell fusion

Particle separation by dielectrophoresis

Dynamic and static cord-structure: A new dielectrophoretic phenomenon

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