Diesel-born organosulfur compounds stimulate community re-structuring in a diesel-biodesulfurizing consortium

Awadh, Maysoon N.; Mahmoud, Huda; Abed, Raeid M. M.; Nayal, Ashraf M. El; Abotalib, Nasser; Ismail, Wael

doi:10.1016/j.btre.2020.e00572

Cited by 15 publications

(11 citation statements)

References 59 publications

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“…Also, the efficiency varies due to the specificity of microorganisms according to the various types of HCS in the crude oil submitted to the biotreatment. Whereas Rhodococcus erythropolis IGTS8 has a tendency of specificity towards DBT more than alkylated forms and BT respectively, Also, each microorganism has special selectivity for which sources of sulfur [ 22 , 32 , 33 ]. Also, the various availability of sources of sulfur compounds generate a competitive in desulfurization, therefore the efficiency with multiple substrates is less than single substrate [ 10 , 26 , 34 ].…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, a model compounds can be applied to lump and represent the whole fossil fuel especially the recalcitrant HCS [13] such as BT, DBT, DBTO2,M DBTSO2, MgSO4, BNT, DBS, 2,8 DMDBT, 2,6 DNDBT, DMDBT, thianthrene [8,[14][15][16][17][18][19][20][21][22][23]. Finally, the microorganisms of BDS applied on water or coal could be common in application of oil [24].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Biodesulfurization of sour heavy crude oil

Al-Khazaali

Ataei

2023

PLoS ONE

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Biodesulfurization of fossil fuels is a promising method for treating the sour oil due to its environmental friendliness and ability to get rid of the recalcitrant organosulfur compounds. In this study, many types of microorganisms such as Ralstonia eutropha, Rhodococcus erythropolis, Acidithiobacillus ferrooxidans, and Acidithiobacillus thiooxidans applied on a sour heavy crude oil (sulfur content was 4.4%). Also, a colony isolated from the crude oil and oil concentrate was examined by supplying it with PTCC 106. The various official and famous mediums were significantly evaluated such as (PTCC 2, PTCC 105, PTCC 106 (9K), PTCC 116, PTCC 123, PTCC 132), sulfur-free MG-medium, basal salts medium, and mineral salts. It was found that Rhodococcus erythropolis and Acidithiobacillus ferrooxidans from microorganisms and SFM and the medium PTCC 105 were selected as the higher desulfurization efficiencies of crude oil equaling 47 and 19.74% respectively. The bioreactions depend on the treated fluid, targeting sulfur compounds as these represent the environmental status (amounts and types of nutrients), and the type of biotreaters whether microorganism are septic, semiseptic, or aseptic. The optimum operation conditions have been designed by using Definitive method such as mixing speed, temperature, surfactant dose, OWR, acidity. The optimum efficiencies obtained here are better than the previous efforts even though those gained by bioengineering. Biodesalination was a simultaneous process with the BDS.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of Biodesulfurization of sour heavy crude oil

Al-Khazaali

Ataei

2023

PLoS ONE

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“…Our findings showed that NR1 desulfurized DBT via the 4S pathway at a 90% of desulfurization rate. Previously reported strains of the T7b can desulfurize 25–59% DBT, including MG1 consortium and Sphingomonas subarctica ( Awadh et al, 2020 ). Overall, we observed that NR1 is a reflective mangrove bacterium in BDS that can degrade organosulfur compounds found in mangrove ecosystems.…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome and Transcriptome Sequencing-Based Characterization of Bacillus Cereus NR1 From Subtropical Marine Mangrove and Its Potential Role in Sulfur Metabolism

Kashif

Sang

et al. 2022

Front. Microbiol.

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Sulfur, organosulfur compounds, and sulfides are essential parts of life. Microbial sulfate assimilation is among the most active and ancient metabolic activities in the sulfur cycle that operates in various ecosystems. We analyzed the molecular basis of bacterial characterization. NR1 was isolated and purified from mangrove sediments. Whole-genome sequencing indicated that the NR1 isolate was closely related to Bacillus cereus. The genome contained 5,305 functional genes with a total length of 5,420,664 bp, a GC content of 35.62%, 42 rRNA, and 107 tRNA. DBT-grown cultures exhibited DBT utilization, fleeting emergence of DBT sulfone (DBTO2), and formation of 2-hydroxybiphenyl (2-HBP). Molecular analysis of the PCR products’ dsz operon revealed the presence of dszA, dszB, and dszC genes, which encoded for NR1’s 90% DBT desulfurization activity. Furthermore, 17 sulfur metabolism-related genes, including genes involved in assimilation sulfate reduction, APS and PAPS, and the cys, ssu, and TST gene families, were identified. In sulfate media, alkenesulfonate was converted to sulfite and inhibited ssu enzymes. Downregulated cysK variants were associated with nrnA expression and the regulation of L-cysteine synthesis. These findings established a scientific foundation for further research and application of bacteria to mangrove rehabilitation and ecological treatment by evaluating the bacterial characterization and sulfur degradation metabolic pathway. We used whole-genome and transcriptome sequencing to examine their genetic characteristics.

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“…IMPS02 is able to remove around 60% of the crude oil's organosulfur from crude oil after incubation at 30 °C for 7 d (Castorena et al 2002). The MG1 consortium and Rhodococcus strain IGTS8 reduced the sulfur content of diesel oil by 25% for 7 d of incubation (Awadh et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Biodesulfurization of the mixture of dibenzothiophene and its alkylated derivatives by Sphingomonas subarctica T7b

Gunam

Sone

Asano

2021

Indones. J. Biotechnol.

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Organosulfur compounds classified as dibenzothiophenes (DBTs) and their derivatives are contained in petroleum. When used as fuel, these substances release SOx emissions, thus contributing to air pollution, acid rain, and climate change. Therefore, it is necessary to reduce the content of these organic sulfur compounds in fuels and one way to achieve this is through bacterial desulfurization. This study reports the biodesulfurization process of a mixture of DBT, 4-hexyl DBT, 4,6-dibutyl DBT, and various organosulfur compounds in light gas oil (LGO). The experiment was conducted by treating 1 mL of aromatic organosulfur compounds with 100 mg/L in \textit{n}-tetradecane or 1 mL LGO with 5 mL mineral salts in sulfur-free medium, incubated at 27 °C for 5 days with shaking at 273 rpm. Gas chromatography analyses revealed that the growing Sphingomonas subarctica T7b cells desulfurized and converted 88.29% of DBT to 2-hydroxybiphenyl as a metabolite while a mixture of DBT and 4,6-dibutyl DBT was desulfurized at 86.40\% and 7.00%, respectively. Furthermore, the mixture of DBT, 4-hexyl DBT, and 4,6-dibutyl DBT had a desulfurization percentage of 84.40%, 41.00%, and 6.66%, respectively, after five days of incubation. The compounds were observed to desulfurize slightly better as single compounds compared to when mixed with other aromatic sulfur compounds.

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Diesel-born organosulfur compounds stimulate community re-structuring in a diesel-biodesulfurizing consortium

Abstract: Highlights A biodesulfurizing consortium consisting of biodesulfurizers and non-biodesulfurizers. Sulfur source-driven compositional shifts in a biodesulfurizing consortium. A biodesulfurizing consortium harbors a non-destructive desulfurization pathway.

Cited by 15 publications

References 59 publications

Optimization of Biodesulfurization of sour heavy crude oil

Optimization of Biodesulfurization of sour heavy crude oil

Whole-Genome and Transcriptome Sequencing-Based Characterization of Bacillus Cereus NR1 From Subtropical Marine Mangrove and Its Potential Role in Sulfur Metabolism

Biodesulfurization of the mixture of dibenzothiophene and its alkylated derivatives by Sphingomonas subarctica T7b

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