Diesel Oil Degradation Potential of a Bacterium Inhabiting Petroleum Hydrocarbon Contaminated Surface Waters and Characterization of Its Emulsification Ability

Abstract

Degradation of poorly water soluble hydrocarbons, like n‐alkanes and polycyclic aromatic hydrocarbons are challenged by some bacteria through emulsification of hydrocarbons by producing biosurfactants. In diesel oil bioremediation, diesel oil degrading and surfactant producing bacteria are used to eliminate these pollutants from contaminated waters. Therefore, identifying and characterizing bacteria capable of producing surfactant and degrading diesel oil are pivotal. In this study, bacteria isolated from hydrocarbon contaminated river water were screened for their potential to degrade diesel oil. Primary selection was carried out by using conventional enrichment culture technique, emulsification index measurement, gravimetric and gas chromatographic analyses of diesel oil degradation. A bacterium with 60 % emulsification index and 92 % diesel oil degradation ability in 14 days was identified as Acinetobacter haemolyticus Zn01 by 16S rRNA sequencing. A. haemolyticus Zn01 was shown to harbor both catabolic genes alkB and C23O effective in diesel oil degradation. The biosurfactant of the bacterium was also characterized in terms of surface tension, zeta potential, Fourier transform infrared spectroscopy and scanning electron microscopy. Being able to emulsify and degrade diesel oil, A. haemolyticus Zn01 seems to have high potential for the elimination of diesel oil from polluted waters.

Effect of spatial origin and hydrocarbon composition on bacterial consortia community structure and hydrocarbon biodegradation rates

“…A 10 mL aliquot was taken and evaporated until constant dryness. Then degradation calculated as explained in Onur, Yilmaz and Icgen ( 2015 ). Degradation of model oil was quantified by separating the extract (1 µL) using gas chromatography (GC; Varian CP3800 fitted with 30 m Zebron ZB-50 column) and individual HCs were detected by a flame ionisation detector (FID).…”

Falsochrobactrum tianjinense sp. nov., a New Petroleum-Degrading Bacteria Isolated from Oily Soils

“…The universal bacterial 16S rRNA primers, 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-CTACGGCTACCTTGTTACGA-3′) were used to amplify bacterial 16S rDNA [ 22 ]. The PCR product of purified strains was subjected to DNA sequencing by the sequencerABI3730-XL.…”

Bioremediation of Petroleum Hydrocarbons Using Acinetobacter sp. SCYY-5 Isolated from Contaminated Oil Sludge: Strategy and Effectiveness Study

“…The bacterial genomic DNA was extracted using the AxyPrep DNA isolation kit. The universal bacterial 16S rDNA primers, 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-CTACGGCTACCTTGTTACGA-3′) were used to amplify bacterial 16S rDNA [ 26 ]. The PCR product of purified strains was subjected to DNA sequencing by the sequencer ABI3730-XL.…”