Falsochrobactrum tianjinense sp. nov., a New Petroleum-Degrading Bacteria Isolated from Oily Soils

Abstract

The microbial remediation technology had great potential and attracted attention to total petroleum hydrocarbon pollution (TPH) remediation, but its efficiency is limited by its application in the field. In this study, a new TPH-degrading strain, TDYN1, was isolated from contaminated oil soil in Dagang Oilfield in Tianjin, China, and identified as Falsochrobactrum sp. by 16S rRNA sequence analysis. The physiological characterization of the isolate was observed. The orthogonal experiment was carried out for the optimum degradation conditions to improve its biodegradation efficiency. The strain was the gram-stain-negative, short rod-shaped, non-spore-forming, designated Falsochrobactrum tianjinense sp. nov (strain TDYN1); it had 3.51 Mb, and the DNA G + C content of the strain was 56.0%. The degradation rate of TDYN1 was 69.95% after 7 days of culture in optimal degradation conditions (temperature = 30 °C, pH = 8, salinity = 10 g L−1, petroleum concentration = 1 g L−1, and the inoculation dose of strain TDYN1 = 6%) and also reached more than 30% under other relatively extreme conditions. It suggested that the TDYN1 has great potential for TPH remediation in the soils of North China.

Isolation and Characterization of Brucella spp., Low-Density Polyethylene (LDPE) Plastic Degrading Bacteria in Al-Ahsa Region, Saudi Arabia

“…The PCR reaction was performed in a 25 µL volume, consisting of 12.5 µL PCR Master Kit (2×), 1 µL of each primer, and 1µL of the DNA template, except for the negative control. Each isolate's 16S rRNA gene was amplified using the universal primers, forward primer (8-F) 5'-AGAGTTTGATCCTGGCTCAG-3', and reverse primer(1492R) 5'-GGTTACCTTGTTACGACTT-3' [37]. In 30 cycles of amplification, the following primary PCR steps were performed: initial denaturation at 94 • C for 5 min, followed by denaturation at 94 • C for 45 s, annealing at 55 • C for 60 s, extension at 72 • C for 60 s, and final extension at 72 • C for 10 min.…”

“…The 16S rRNA gene for bacterial isolates was sequenced on an automated DNA sequencing machine made by Applied Biosystems (model 3730XL), then compared with those in the GenBank database using the BLAST algorithm (National Center for Biotechnology Information, Bethesda, MD, USA). Phylogenetic trees were constructed using the Mega7 program, using the neighbor-joining method based on the Tamura-Nei model [37,39]. The capacity of pure APCK5 and APCZ14 to break down LDPE plastic was studied using synthetic medium containing LDPE as the sole source of energy and carbon.…”

Molecular and metabolic characterization of petroleum hydrocarbons degrading Bacillus cereus

“…strain TY-5, Acinetobacter sp. and, Falsochrobactrum tianjinense , respectively ( Hu et al 2022 ; Wang et al 2023 ). Additionally, petroleum aromatic compounds degradation mechanisms have also been reported in literature like o-ring cleavage and m-ring cleavage pathways, toluene 4-monooxygenase pathway (TMO), toluene 3-monooxygenase pathway (Tbu), toluene 2-monooxygenase pathway (TOM), toluene dioxygenase pathway (Tod), toluene methyl monooxygenase pathway (TOL) for toluene, salicylate pathway, gentisate pathway, procatechuic acid pathways for naphthalene, anaerobic benzene degradation pathways via methylation, hydroxylation and carboxylation, xylene degradation pathways via phenol hydroxylase, monooxygenase and dioxygenase routes, β-ketoadipic acid pathway of benzoic acid degradation and the novel pathways of toluene and naphthalene degradation involving the cyclohexene, cyclohexane, cyclohexanol and cyclohexanone formation ( Reiner 1971 ; Denome et al 1993 ; Goyal and Zylstra 1997 ; Abu Laban et al 2010 ; Brzeszcz and Kaszycki 2018 ; Di Canito et al 2018 ; Muccee et al 2019 ; Muccee et al 2021 ).…”