Diet-delivered RNAi in Helicoverpa armigera – Progresses and challenges

Lim, Zhi Xian; Robinson, Karl E.; Jain, Ritesh G.; Chandra, G. Sharath; Asokan, R.; Asgari, Sassan; Mitter, Neena

doi:10.1016/j.jinsphys.2015.11.005

Cited by 44 publications

(37 citation statements)

References 95 publications

(103 reference statements)

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“…Further increase in dsRNAs did not significantly increase silencing efficiency, indicating that the concentration was saturated once it passed a certain threshold. Saturation of the silencing effect has also been reported in other insects; for example, saturation in H. armigera was reported at 53.125 µg dsRNA day −1 …”

Section: Discussionsupporting

confidence: 58%

“…Saturation of the silencing effect has also been reported in other insects; for example, saturation in H. armigera was reported at 53.125 μg dsRNA day −1 . 26,42 The success of RNAi-based strategy in future pest management relies on the identification of essential target genes that confer a lethal phenotypic effect upon knockdown of the intended gene. 61 Generally, functional genes involved in insect herbivory, development, metamorphosis or key metabolic processes could be suitable RNAi targets as part of insect control strategies.…”

Section: Discussionmentioning

confidence: 99%

“…Since its first discovery in Caenorhabditis elegans , gene knockdown through dsRNA‐mediated RNAi has been widely applied for genetic research that includes the economically important insect orders Lepidoptera, Coleoptera and hemipteran pests such as aphids, whitefly and psyllids . Owing to its high sequence‐dependent specificity and wide range of gene targets for exploitation, RNAi‐mediated insect control is considered as the next‐generation pest management strategy with benefits over chemical‐based pesticides or traditional biological control . Efficient dsRNA delivery and suitable RNAi targets are the two prerequisites for RNAi‐mediated insect management .…”

Section: Introductionmentioning

confidence: 99%

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Double‐stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri

Gowda

Killiny

2017

Pest Management Science

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Double‐stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri

Gowda

Killiny

2017

Pest Management Science

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“…mori was not successful in this study. RNAi efficiencies of some genes have been obtained in some lepidopteran insects (Tian et al, 2009;Lim et al, 2016). However, the RNAi efficiencies of a lot of genes in Lepidoptera are far inferior to those in other species owing to a strong resistance mechanism and unknown reasons (Terenius et al, 2011;Xu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

20‐Hydroxyecdysone receptor‐activated Bombyx mori CCAAT/enhancer‐binding protein gamma regulates the expression of BmCBP and subsequent histone H3 lysine 27 acetylation in Bo. mori

Lyu

Chen

et al. 2020

Insect Molecular Biology

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Cyclic adenosine monophosphate (cAMP) response element binding protein (CREB)‐binding protein (CBP or CREBBP) plays important roles in regulating gene transcription and animal development. However, the process by which CBP is up‐regulated to impact insect development is unknown. In this study, the regulatory mechanism of Bombyx mori CBP (BmCBP) expression induced by 20‐hydroxyecdysone (20E) was investigated. In the Bo. mori cell line, DZNU‐Bm‐12, 20E enhanced BmCBP transcription and histone H3K27 acetylation. BmCBP RNA interference (RNAi) resulted in decreased histone H3K27 acetylation. Additionally, the luciferase activity analysis revealed that the transcription factor, Bo. mori CCAAT/enhancer‐binding protein gamma (BmC/EBPg), activated BmCBP transcription, which was suppressed by BmC/EBPg RNAi and promoted by BmC/EBPg overexpression. Electrophoretic mobility shift assay and chromatin immunoprecipitation results demonstrated that BmC/EBPg could bind to the C/EBP cis‐regulatory elements in two positions of the BmCBP promoter. Moreover, BmC/EBPg transcription was enhanced by the 20E receptor (BmEcR), which bound to the BmC/EBPg promoter. BmEcR RNAi significantly inhibited the transcriptional levels of BmC/EBPg and BmCBP in the presence of 20E. Furthermore, the BmEcR‐BmC/EBPg pathway regulated the acetylation levels of histone H3K27. Altogether, these results indicate that BmEcR enhances the expression of BmC/EBPg, which binds to the BmCBP promoter, activates BmCBP expression and leads to histone H3K27 acetylation.

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“…It is also possible that RNAi can spread without the need for amplification, if sufficient dsRNA is continuously taken up. Indeed, single high‐dose feeding of dsRNA was less effective than continuous diet‐based application in Coleoptera . There is clear evidence that the RNAi response can be systemic in insects lacking a canonical RdRp .…”

Section: Rnai Environmental Rna and Trans‐kingdom Rnaimentioning

confidence: 99%

Improved insect‐proofing: expressing double‐stranded RNA in chloroplasts

Bally¹,

Fishilevich²,

Bowling³

et al. 2018

Pest Management Science

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RNA interference (RNAi) was discovered almost 20 years ago and has been exploited worldwide to silence genes in plants and animals. A decade later, it was found that transforming plants with an RNAi construct targeting an insect gene could protect the plant against feeding by that insect. Production of double‐stranded RNA (dsRNA) in a plant to affect the viability of a herbivorous animal is termed trans‐kingdom RNAi (TK‐RNAi). Since this pioneering work, there have been many further examples of successful TK‐RNAi, but also reports of failed attempts and unrepeatable experiments. Recently, three laboratories have shown that producing dsRNA in a plant's chloroplast, rather than in its cellular cytoplasm, is a very effective way of delivering TK‐RNAi. Our review examines this potentially game‐changing approach and compares it with other transgenic insect‐proofing schemes. © 2018 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Diet-delivered RNAi in Helicoverpa armigera – Progresses and challenges

Cited by 44 publications

References 95 publications

Double‐stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri

Double‐stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri

20‐Hydroxyecdysone receptor‐activated Bombyx mori CCAAT/enhancer‐binding protein gamma regulates the expression of BmCBP and subsequent histone H3 lysine 27 acetylation in Bo. mori

Improved insect‐proofing: expressing double‐stranded RNA in chloroplasts

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