Dietary Berries and Ellagic Acid Prevent Oxidative DNA Damage and Modulate Expression of DNA Repair Genes

Aiyer, Harini S.; Vadhanam, Manicka V.; Stoyanova, Radka; Caprio, Gerard D.; Clapper, Margie L.; Gupta, Ramesh C.

doi:10.3390/ijms9030327

Cited by 60 publications

(48 citation statements)

References 41 publications

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“…Pterostilbene, primarily found in blueberries, is an antioxidant that acts as an effective anticancer agent in various common malignancies, including breast and colon cancer (20). In addition, ellagic acid has been associated with the prevention of oxidative DNA damage and modulation of DNA repair gene expression (21). These components interact and influence biological processes in the human body, resulting in the potentially protective and preventive actions of blueberries.…”

Section: Discussionmentioning

confidence: 99%

Effects of blueberries on migration, invasion, proliferation, the cell cycle and apoptosis in hepatocellular carcinoma cells

Zhan

Liao

et al. 2016

Biomedical Reports

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Abstract. The aim of the present study was to investigate the effects of blueberry consumption on the migration, invasion, proliferation, cell cycle and apoptosis in human hepatocellular carcinoma (HCC) cells, in order to provide clinical treatment and prevention strategies for liver cancer using anticancer therapeutic agents. Rabbiteye blueberry was prepared as fresh juice and fed to rats at low, moderate and high dosages (25, 50 and 100%, respectively) by daily gastric gavage. Seven days later, the rats were sacrificed and the blood serum was obtained for co-culture with HEPG2 cells. The MTT assay was used for detecting cell proliferation, Transwell assay was performed for migration and invasion evaluation, and cell cycle and apoptosis were assessed by flow cytometry. After co-culturing with the blood serum of rats that were fed different dosages of blueberry juice, the inhibition rate of HEPG2 cells in the three groups was significantly lower than that in the control group at 48 and 72 h (P<0.05). The number of migrated and transmembrane HEPG2 cells in the three groups was significantly lower than that in the control group at 48 and 72 h (P<0.05). The number of migrated HEPG2 cells in the high dosage group was significantly lower than that in the low dosage group at 48 h, and the numbers of migrated HEPG2 cells in the high and moderate dosage groups were significantly lower than that in the low dosage group at 72 h (P<0.05). The number of transmembrane HEPG2 cells in the high dosage group was significantly lower than that in the low dosage group at 48 h (P<0.05). The numbers of HEPG2 cells at the G 2 /M stage in the three groups were significantly lower than that in the control group, and the number of HEPG2 cells in the high dosage group was significantly lower than that in the low dosage group, at 48 and 72 h (P<0.05). The apoptosis rate in the three groups was significantly higher than that in the control group, and the apoptosis rate in the high dosage group was significantly higher than that in the low dosage group at 48 and 72 h (P<0.05). Thus, blueberries may facilitate the clinical treatment of HCC, providing a novel therapeutic and prevention strategy for HCC as an anticancer therapeutic agent.

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Section: Discussionmentioning

confidence: 99%

Effects of blueberries on migration, invasion, proliferation, the cell cycle and apoptosis in hepatocellular carcinoma cells

Zhan

Liao

et al. 2016

Biomedical Reports

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“…Aiyer et al [49] fed mice a diet containing either ellagic acid (a polyphenol found in many berries), or dehydrated blueberry, strawberry or raspberry. Ellagic acid and raspberry had particularly strong effects, increasing mRNA levels of Xpa, Ercc5 and Dnl3 (DNA ligase III) by three-to eightfold.…”

Section: Animal Studies Of the Effect Of Micronutrients On Dna Repairmentioning

confidence: 99%

Effects of micronutrients on DNA repair

2012

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“…For the preparations of the BB and BRB diet, AIN 93M diet was powdered and mixed with BB and BRB powder at 5% and 2.5% (w/w), respectively, and stored at 4 • C until use. The diets were isocaloric and the recipe used was the same as described by us elsewhere [8,16,17].…”

Section: Dietmentioning

confidence: 99%

Lung cancer inhibitory activity of dietary berries and berry polyphenolics

Aqil

Jeyabalan

Kausar

et al. 2016

JBR

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Abstract.BACKGROUND: Blueberry (BB) and black raspberry (BRB) have been shown to be chemopreventive against estrogenmediated breast cancer in pre-clinical studies. However, therapeutic efficacy of these berries against lung cancer is not known. METHODS: In this study we investigated i) relative efficacy of individual anthocyanidins vs. respective anthocyanins, ii) relative antiproliferative activity of mixture of anthocyanidins compared to individual anthocyanidins, iii) antitumor activity of dietary BB, iv) Tumor inhibitory activity of diet supplemented with BB, alone and in combination with BRB, against lung tumor xenograft using nude mice, and finally, v) the efficacy of select polyphenolics present in BB and BRB against lung tumor xenograft. RESULTS: Our findings indicated that individual anthocyanidins (aglycones) were significantly more potent (2-3 fold lower IC 50 ) in inhibiting the non-small-cell lung cancer (NSCLC) cell growth vs. respective anthocyanins (glycones). Further, anthocyanidins mixture at equimolar concentrations exhibited synergistic antiproliferative activity vs. individual anthocyanidins. When tested against NSCLC (A549 and H1299) cells in nude mice, dietary BB (7.5%, w/w) showed >40% reduction in tumor volume against H1299 xenografts. The maximal growth inhibition occurred with 5% BB dose, with no additional protection occurring at a higher dose (7.5%). However, somewhat lower protection was found when the BB diet initiated prior to tumor cell inoculation. The mixture of BB (5%, w/w) and BRB (2.5%) resulted in higher inhibition of tumor growth vs. BB alone (71% vs 42%). Likewise, a combination of delphinidin (bioactive of BB) and punicalagins (a bioactive of BRB, which gets converted to ellagic acid in vivo) showed higher tumor growth inhibition compared to delphinidin. CONCLUSIONS: The therapeutic effects of the berries and berry polyphenolics observed against lung cancer in this study are highly encouraging. Further investigation into the mechanism of action of the combinations of the berry bioactives will be valuable for clinical use of this potent natural product against lung cancer.

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Dietary Berries and Ellagic Acid Prevent Oxidative DNA Damage and Modulate Expression of DNA Repair Genes

Cited by 60 publications

References 41 publications

Effects of blueberries on migration, invasion, proliferation, the cell cycle and apoptosis in hepatocellular carcinoma cells

Effects of blueberries on migration, invasion, proliferation, the cell cycle and apoptosis in hepatocellular carcinoma cells

Effects of micronutrients on DNA repair

Lung cancer inhibitory activity of dietary berries and berry polyphenolics

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