Dietary Beta-Hydroxy-Beta-Methyl Butyrate Supplementation Affects Growth, Carcass Characteristics, Meat Quality, and Serum Metabolomics Profile in Broiler Chickens

Abstract

This study aimed to explore the effects of beta-hydroxy-beta-methyl butyrate (HMB) on serum metabolic profiles and meat quality of muscles in Wenshi broiler chickens. Birds were fed a basal diet with an additional 0, 0.05, 0.10, or 0.15% HMB, respectively. Results showed that dietary HMB quadratically increased the average daily gain (P = 0.058) and decreased feed:gain (P < 0.05) mainly in the starter phase. At 51 days of age, birds receiving 0.10% HMB diet exhibited less abdominal fat and more breast yield than the control (P < 0.05). Moreover, dietary HMB quadratically decreased the L∗ value and drip loss in selected muscles (P < 0.05) and increased the a∗ value in breast muscle (P < 0.05). Serum metabolome profiling showed that the most differentially abundant metabolites are lipids and lipid-like molecules, including phosphatidylcholines. It was concluded that HMB improved growth performance and meat quality of muscle in broilers.

Dietary Beta-Hydroxy-Beta-Methyl Butyrate Supplementation Inhibits Hepatic Fat Deposition via Regulating Gut Microbiota in Broiler Chickens

“…Moreover, accumulating and emerging lines of evidence have revealed a positive association between the microbiota diversity in cecum, where the chyme is largely fermented, and growth performance in broilers [43,44]. Similarly in our study, we found that higher richness of microbiota in cecum is accompanied by higher growth performance in broilers [14]. Furthermore, we performed PICRUSt analysis to reveal alterations in metabolism triggered by these bacteria, and demonstrated that carbohydrate transport and metabolism was the primary factor that promoted growth.…”

“…The HMB was obtained from Jiangyin TSI Pharmaceutical Co., Ltd. (Shanghai, China). The diet composition is presented in Supplementary Table S1 [14]. During the rearing period, all broilers were housed in an environmentally controlled house.…”

“…On day 51, 12 broilers (two broilers per replicate, n = 6/treatment) of each treatment were randomly selected and killed after a 12 h fast to collect blood samples [14]. The abdominal fat and liver tissue samples were collected and weighed.…”

“…In addition, slaughter performance in poultry can be improved by effectively regulating the expression of lipid metabolism-related genes in the liver [11,12]. Previous studies have demonstrated that β-hydroxy-β-methylbutyrate (HMB), a metabolite of leucine, could augment body weight gain and slaughter performance in broiler chickens [13,14]. Moreover, recent evidence from rodent and swine models suggests that HMB administration could reduce fat deposition [15,16].…”

Synergistic Effects of Lotus Seed Resistant Starch and Sodium Lactate on Hypolipidemic Function and Serum Nontargeted Metabolites in Hyperlipidemic Rats

“…The instrumental platform for LC-MS analysis was an ultrahigh performance liquid chromatography tandem Fourier transform mass spectrometry UHPLC-Q Exactive HF-X system (Thermo Fisher) according to the previous method . The following chromatographic conditions were used: The column was an ACQUITY UPLC HSS T3 (100 mm × 2.1 mm i.d., 1.8 μm; Waters, Milford, USA).…”