Dietary calcium supplementation in adult rats reverts brown adipose tissue dysfunction programmed by postnatal early overfeeding

Conceição, Ellen Paula Santos da; Moura, Egberto Gaspar de; Oliveira, Elaine de; Guarda, Deysla Sabino; Figueiredo, Mariana Sarto; Quitete, Fernanda Torres; Calvino, Camila; Miranda, Rosiane Aparecida; Mathias, Paulo Cézar de Freitas; Manhães, Alex C.; Lisboa, Patrícia Cristina

doi:10.1016/j.jnutbio.2016.09.013

Cited by 16 publications

(19 citation statements)

References 55 publications

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“…Also, calcium supplementation increases intracellular calcium concentration to regulate the activity of Fasn and hormone sensitive lipases, which results in a reduction of fat storage and increases in fat decomposition and oxidation 19 . Dietary calcium supplementation is reported to be able to improve cardiovascular parameters and brown adipose tissue thermogenesis capacity in adult animals that were early overfed during lactation 23 . Studies in mice have shown that an increase in dietary calcium consumption creates a shift in the utilization of energy stores from carbohydrates to fat and a shift in the partitioning of energy from storage (as fat) to expenditure (as heat) 8 , 23 .…”

Section: Discussionmentioning

confidence: 99%

“…Dietary calcium supplementation is reported to be able to improve cardiovascular parameters and brown adipose tissue thermogenesis capacity in adult animals that were early overfed during lactation 23 . Studies in mice have shown that an increase in dietary calcium consumption creates a shift in the utilization of energy stores from carbohydrates to fat and a shift in the partitioning of energy from storage (as fat) to expenditure (as heat) 8 , 23 . Other mechanisms may also be involved, because calcium imbalance may change secretion of parathyroid hormone and 1,25-dihydroxyvitamin D concentration, which regulated adipocyte activity and lipolysis 24 , 25 .…”

Section: Discussionmentioning

confidence: 99%

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Dietary calcium status during maternal pregnancy and lactation affects lipid metabolism in mouse offspring

Chang

Fan

et al. 2018

Sci Rep

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Calcium plays important roles in lipid metabolism and adipogenesis, but whether its status in early life affects later lipid profiles needs to be clarified. Three to four-week old C57BL/6J female mice were fed with three different reproductive diets containing normal, low (insufficient) and high (excessive) calcium concentrations respectively throughout pregnancy and lactation. At postnatal 21 days, the weaning male and female pups from each group were sacrificed for experiments and the remaining were fed with the normal chow diet for 16 weeks. Meanwhile, some of the weaning female pups from maternal low calcium diet group were fed with the normal calcium, low calcium and high calcium mature diets respectively for 8 weeks. Maternal insufficient or excessive calcium status during pregnancy and lactation programmed an abnormal expression of hepatic and adipose genes (PPAR-γ, C/EBP-α, FABP4, Fasn, UCP2, PPAR-α, HMG-Red1, Acc1, and SREBP-1c) in the offspring and this may lead to dyslipidemia and accumulation of hepatic triglyceride (TG) and total cholesterol (TC) in later life. The effects of maternal calcium status on lipid metabolism were found only in the female adult offspring, but were similar between offspring males and females at postnatal 21 days. Additionally, the dyslipidemia and hepatic lipid accumulation caused by insufficient calcium status in early life may be reversed to some extent by dietary calcium supplementation in later life.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dietary calcium status during maternal pregnancy and lactation affects lipid metabolism in mouse offspring

Chang

Fan

et al. 2018

Sci Rep

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“…Intracellular calcium chelator, BAPTA‐AM enhanced insulin‐activated protein–protein interaction, PI3K activity and adipogenesis (Worrall and Olefsky, ; Sun et al, ). Changes in [Ca 2+ ] i have also been reported to be involved in energy metabolism of BAT and beige adipocytes in sWAT (Klepac et al, ; Conceicao et al, ). In the present study, we focused on the non‐selective calcium‐permeable cation channels, thermoTRPs which are activated by temperatures at physiological ranges in iBAT and sWAT.…”

Section: Discussionmentioning

confidence: 99%

Gene expression changes of thermo‐sensitive transient receptor potential channels in obese mice

Sun

Zhang

et al. 2017

Cell Biology International

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Adipose tissues play key roles in energy homeostasis. Brown adipocytes and beige adipocytes in white adipose tissue (WAT) share the similar characters of thermogenesis, both of them could be potential targets for obesity management. Several thermo-sensitive transient receptor potential channels (thermoTRPs) are shown to be involved in adipocyte biology. However, the expression pattern of thermoTRPs in adipose tissues from obese mice is still unknown. The mRNA expression of thermoTRPs in subcutaneous WAT (sWAT) and interscapular brown adipose tissue (iBAT) from lean and obese mice were measured using reverse transcriptase-quantitative PCRs (RT-qPCR). The results demonstrated that all 10 thermoTRPs are expressed in both iBAT and sWAT, and without significant difference in the mRNA expression level of thermoTRPs between these two tissues. Moreover, Trpv1 and Trpv3 mRNA expression levels in both iBAT and sWAT were significantly decreased in high fat diet (HFD)-induced obese mice and db/db (leptin receptor deficient) mice. Trpm2 mRNA expression level was significantly decreased only in sWAT from HFD-induced obese mice and db/db mice. On the other hand, Trpv2 and Trpv4 mRNA expression levels in iBAT and sWAT were significantly increased in HFD-induced obese mice and db/db mice. Taken together, we conclude that all 10 thermoTRPs are expressed in iBAT and sWAT. And several thermoTRPs differentially expressed in adipose tissues from HFD-induced obese mice and db/db mice, suggesting a potential involvement in anti-obesity regulations.

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“…At PND180, one animal from each litter per group (C and SE) was fasted for 12 h and then anesthetized (ketamine 70 mg/kg and xylazine 7 mg/kg) for in vivo autonomic nerve electrical activity assessment as previously described ( 24 ). Interscapular BAT sympathetic nerve activity (SNA) from the left interscapular nerve was exposed under a dissection microscope.…”

Section: Methodsmentioning

confidence: 99%

“…Animals were kept under a warming light. After 10 min of stabilization, the average number of spikes per 10 s intervals during a 10-min period was calculated ( 24 ). The background noise level was determined in a nerve segment.…”

Section: Methodsmentioning

confidence: 99%

Neonatal tobacco smoke reduces thermogenesis capacity in brown adipose tissue in adult rats

Peixoto

Moura

Oliveira

et al. 2018

Braz J Med Biol Res

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Maternal smoking is a risk factor for progeny obesity. We have previously shown, in a rat model of neonatal tobacco smoke exposure, a mild increase in food intake and a considerable increase in visceral adiposity in the adult offspring. Males also had secondary hyperthyroidism, while females had only higher T4. Since brown adipose tissue (BAT) hypofunction is related to obesity, here we tested the hypothesis that higher levels of thyroid hormones are not functional in BAT, suggesting a lower metabolic rate. We evaluated autonomic nerve activity in BAT and its function in adult rats that were exposed to tobacco smoke during lactation. At birth, litters were adjusted to 3 male and 3 female pups/litter. From postnatal day (PND) 3 to 21, Wistar lactating rats and their pups were divided into SE group, smoke-exposed in a cigarette smoking machine (4 times/day) and C group, exposed to filtered air. Offspring were sacrificed at PND180. Adult SE rats of both genders had lower interscapular BAT autonomic nervous system activity, with higher BAT mass but no change in morphology. BAT UCP1 and CPT1a protein levels were decreased in the SE groups of both genders. Male SE rats had lower β3-AR, TRα1, and TRβ1 expression while females showed lower PGC1α expression. BAT Dio2 mRNA and hypothalamic POMC and MC4R levels were similar between groups. Hypothalamic pAMPK level was higher in SE males and lower in SE females. Thus, neonatal cigarette smoke exposure induces lower BAT thermogenic capacity, which can be obesogenic at adulthood.

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Dietary calcium supplementation in adult rats reverts brown adipose tissue dysfunction programmed by postnatal early overfeeding

Cited by 16 publications

References 55 publications

Dietary calcium status during maternal pregnancy and lactation affects lipid metabolism in mouse offspring

Dietary calcium status during maternal pregnancy and lactation affects lipid metabolism in mouse offspring

Gene expression changes of thermo‐sensitive transient receptor potential channels in obese mice

Neonatal tobacco smoke reduces thermogenesis capacity in brown adipose tissue in adult rats

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