Dietary carbohydrate effects on histological features of ileal mucosa in White Leghorn chicken

Abstract

White Leghorn chickens were divided into the control , lowcarbohydrate (CHO), and CHO-free groups to investigate dietary CHO's significance on histological features of chicken ileal mucosa. Paraffin sections of distal ileum from each chicken were stained by periodic acid-Schiff reaction and subjected to morphometrical analysis. Most villi in the control group had a fingerlike shape but those of the experimental groups showed irregular shapes. Villus height, crypt depth and the number of mitotic cells per crypt were significantly lower in the CHO-free group than in the control group. The density of goblet cells also showed a significant decreasing trend with a reduction in dietary CHO level. In conclusion, dietary CHO positively affects the proliferation of epithelial cells in the chicken ileum.

Polar lipid-enriched milk fat globule membrane supplementation in maternal high-fat diet promotes intestinal barrier function and modulates gut microbiota in male offspring

“…This journal is © The Royal Society of Chemistry 2023 shallower the crypt, the better the maturity of cells, resulting in a better secretion function. 37 Therefore, the V/C ratio positively correlates with the functional state of the small intestine. 38 Mice fed with HFD had a decreased V/C ratio in the small intestine and maternal HFD disrupted the structure of the mucosal epithelium in offspring.…”

In vitro Digestion Characteristics of Hydrolyzed Infant Formula and Its Effects on the Growth and Development in Mice

“…The digestion and absorption of nutrients in mammals mainly occur in the small intestine. Villus height corresponds to the nutrient absorption area and crypt depth corresponds to intestinal epithelium turnover, so villus height and crypt depth are regarded as indicators of intestinal health and development (51). The integrity, uniformity, and compactness of the intestines of the mice in the pHIF and eHIF groups were significantly better than those in the control and sIF groups (Figure 5D).…”

“…In addition, the villus height and crypt depth in the eHIF group were significantly better than those in the control, sIF, and pHIF groups (p < 0.05) (Figures 5E,F). Higher villi enhanced the surface area of the lumen available for absorption, increased the action of digestive enzymes, and sped up nutrient transport; whereas deeper crypts indicated the rapid turnover of cells, such as absorbing, secreting, and regenerating cells (51). Therefore, eHIF could promote the development of the small intestine in mice.…”