2011
DOI: 10.1152/ajpregu.00579.2010
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Dietary carbohydrate-to-protein ratio affects TOR signaling and metabolism-related gene expression in the liver and muscle of rainbow trout after a single meal

Abstract: Most teleost fish are known to require high levels of dietary proteins. Such high-protein intake could have significant effects, particularly on insulin-regulated gene expression. We therefore analyzed the effects of an increase in the ratio of dietary carbohydrates/proteins on the refeeding activation of the Akt-target of rapamycin (TOR) signaling pathways in rainbow trout and the effects on the expression of several genes related to hepatic and muscle metabolism and known to be regulated by insulin, amino ac… Show more

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Cited by 117 publications
(132 citation statements)
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“…Moreover, this induction of activity is mainly linked to the induction of GK gene expression in the liver. The increased GK gene expression in the liver of carnivorous fish appears after long-term feeding with carbohydrates but also after a single meal with carbohydrates, as shown in rainbow trout (204,209,210) ( Figs. 3 and 4).…”
Section: Nutritional Regulation Of Hepatic Glucokinasementioning
confidence: 74%
“…Moreover, this induction of activity is mainly linked to the induction of GK gene expression in the liver. The increased GK gene expression in the liver of carnivorous fish appears after long-term feeding with carbohydrates but also after a single meal with carbohydrates, as shown in rainbow trout (204,209,210) ( Figs. 3 and 4).…”
Section: Nutritional Regulation Of Hepatic Glucokinasementioning
confidence: 74%
“…Recent studies performed in mammals and fish indicate that TOR signalling pathway controls de novo lipogenesis by regulating the expression and processing of sterol regulatory element-binding protein 1c (SREBP1c), a master regulator of sterol-and lipogenic gene transcription (Dentin et al 2005;Duvel et al 2010;Eberle et al 2004;Lansard et al, 2010;Seiliez et al 2011). Genes that code the enzymes glucokinase (GK), ATP-citrate lyase (acyl), acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) have been thus identified as targets of the transcription factor SREBP-1c (Girard et al 1997;Kim 2004;Portsmann et al 2008;Sato 2000;.…”
Section: Discussionmentioning
confidence: 99%
“…As such, our data confirm the link between the mTOR pathway and SREBP-1c as the master regulator of fatty acid and triglyceride synthesis in barramundi, resulting in increased expression of glycolytic and lipogenic genes. Whereas Akt phosphorylation is mainly controlled by insulin in trout, in vivo activation of the mTOR pathway seems to require both insulin and amino acids (Avruch et al, 2009;Lansard et al 2010 and2011;Seiliez et al 2011). We observed a small increase in circulating free amino acids in the plasma that coincided with the activation of the mTOR pathway, but this was not significantly correlated.…”
Section: Discussionmentioning
confidence: 99%
“…The primer sequences used in the quantitative real-time PCR, as well as the protocol conditions of the assays, have been previously published. 47,48 Primers of the Plin2/Adrp, Plin3/Tip47, Atp6v1a and Ddit3 genes were newly designed using Primer3 software, as previously described. 49 To confirm specificity of the newly developed RT-PCR assay, the amplicon was purified and sequenced (Beckman-Coulter Genomics, Takeley, UK).…”
Section: Mrna Levels Analysis: Quantitative Rt-pcrmentioning
confidence: 99%