Dietary docosahexaenoic acid is more optimal than eicosapentaenoic acid affecting the level of cellular defence responses of the juvenile grouper Epinephelus malabaricus

Wu, Feng-Cheng; Ting, Yun-Yuan; Chen, H.Y.

doi:10.1006/fsim.2002.0433

Cited by 52 publications

(30 citation statements)

References 55 publications

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“…Fatty acid composition of fish muscle lipids usually reflects that of the lipids in their feed [19,[52][53][54][55]. Our results are in agreement with previous reports that dietary EPA and DHA concentrations show good correlations with tissue concentrations of DHA and EPA [56].…”

Section: Discussionsupporting

confidence: 94%

Different Ratios of Docosahexaenoic and Eicosapentaenoic Acids Do Not Alter Growth, Nucleic Acid and Fatty Acids of Juvenile Cobia (Rachycentron canadum)

Xu¹,

Ding²,

Zhang³

et al. 2009

Lipids

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An experiment was performed to study the effect of different ratios of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on the growth, nucleic acid and fatty acids of cobia (Rachycentron canadum) juveniles. The juveniles were fed for 8 weeks using seven treatment diets (D-1-D-7) with the same amount of DHA and EPA (1.50 +/- 0.1% of dried diet), but varying ratios of DHA to EPA (0.90, 1.10, 1.30, 1.50, 1.70, 1.90, 2.10, respectively) and a control diet (D-0, DHA + EPA = 0.8% of dried diet, DHA/EPA = 1.30). At the end of the experiment, the mean body weight (BW) of juveniles fed D-0-D-7 increased significantly (from 6.86 +/- 1.64 in the week 0 to 58.52 +/- 16.45 g at the end of week 8, P < 0.05). The mean RNA amount and RNA/DNA ratio in the muscle (from 39.62 +/- 1.30 microg mg(-1) and 2.29 +/- 0.11 in the week 0 to 272.55 +/- 10.70 microg mg(-1) and 14.54 +/- 1.75 at the end of week 8, respectively) and the mean weight in the liver (from 117.70 +/- 11.15 microg mg(-1) and 3.14 +/- 0.25 in the week 0 to 793.07 +/- 13.38 microg mg(-1) and 13.16 +/- 0.76 at the end of week 8, respectively) of cobia juveniles fed D-0-D-7 were significantly higher at the end of 8-week experiment than initially (P < 0.05). The RNA/DNA ratio in the muscle and liver of cobia juveniles increased with their growth and appeared an obvious positive relationship, especially in the muscle, based on regression analysis. The mean lipid content increased significantly in the liver (from 29.82 +/- 0.99 to 37.47 +/- 3.25% totally) and muscle (from 6.74 +/- 0.25 to 10.63 +/- 0.23% totally) of cobia juveniles (P < 0.05). However, no significant difference was found on the lipid contents of juveniles fed different diets for 8 weeks (P > 0.05). In the muscle and liver of juveniles, EPA decreased with its reduction in the diet; DHA, DHA/EPA ratio and poly unsaturated fatty acids (PUFAs) generally increased with their increment in the diet. The conclusion was drawn that the growth, nucleic acid and fatty acids of cobia juveniles were not significantly affected by different DHA/EPA ratios in our experiments.

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Section: Discussionsupporting

confidence: 94%

Different Ratios of Docosahexaenoic and Eicosapentaenoic Acids Do Not Alter Growth, Nucleic Acid and Fatty Acids of Juvenile Cobia (Rachycentron canadum)

Xu¹,

Ding²,

Zhang³

et al. 2009

Lipids

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“…The inclusion of the two fatty acids in fish diets are known to improve the quality of broodstocks, and the growth and normal development of larvae (Watanabe, 1983;Furuita et al, 2000;Copeman et al, 2002;Mazorra et al, 2003;Watanabe & Vassallo-Agius, 2003). Moreover, the highly unsaturated fatty acids have been shown to promote tolerance to handling stress (Koven et al, 2001), increase cellular (Wu et al, 2003) and humoral (Kiron et al, 1995) defense responses and offer disease resistance (Kiron et al, 1995). These fatty acids also enhanced the schooling behavior of the larval yellowtail (Ishizaki et al, 2001).…”

Section: Resultsmentioning

confidence: 96%

Enhancement of EPA and DHA biosynthesis by over-expression of masu salmon Δ6-desaturase-like gene in zebrafish

et al. 2005

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The n - 3 polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have important nutritional benefits in humans. Farmed fish could serve as promising sources of EPA/DHA, but they need these fatty acids or their precursors in their diets. Here we transferred masu salmon delta6-desaturase-like gene in zebrafish to increase its ability for synthesizing EPA and DHA. Expression of this gene in transgenic fish elevated their EPA content by 1.4-fold and DHA by 2.1-fold. On the other hand, the alpha-linolenic acid (ALA) content decreased, it being a substrate of delta6-desaturase, while the total lipid remained constant. This achievement demonstrates that fatty acid metabolic pathway in fish can be modified by the transgenic technique, and perhaps this could be applied to tailor farmed fish as even better sources of valuable human food.

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“…NBT positive cell percentage and SOD play a critical role in controlling the balance of release and clear of ROS in immune cells, particularly during the immune processes such as phagocytosis, and hence are indicative parameters of immune functions [42e44]. A previous study found that phagocytic function was enhanced by appropriate supplementation of dietary n-3 HUFA [45]. However, PI showed no significant differences among dietary treatments in the present study, which may be due to immune fatigue caused by parasite infection.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary n-3 highly unsaturated fatty acids on growth, nonspecific immunity, expression of some immune related genes and disease resistance of large yellow croaker (Larmichthys crocea) following natural infestation of parasites (Cryptocaryon irritans)

Zuo

Mai

et al. 2012

Fish & Shellfish Immunology

206

102

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Dietary docosahexaenoic acid is more optimal than eicosapentaenoic acid affecting the level of cellular defence responses of the juvenile grouper Epinephelus malabaricus

Cited by 52 publications

References 55 publications

Different Ratios of Docosahexaenoic and Eicosapentaenoic Acids Do Not Alter Growth, Nucleic Acid and Fatty Acids of Juvenile Cobia (Rachycentron canadum)

Different Ratios of Docosahexaenoic and Eicosapentaenoic Acids Do Not Alter Growth, Nucleic Acid and Fatty Acids of Juvenile Cobia (Rachycentron canadum)

Enhancement of EPA and DHA biosynthesis by over-expression of masu salmon Δ6-desaturase-like gene in zebrafish

Effects of dietary n-3 highly unsaturated fatty acids on growth, nonspecific immunity, expression of some immune related genes and disease resistance of large yellow croaker (Larmichthys crocea) following natural infestation of parasites (Cryptocaryon irritans)

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