Dietary fat alters the expression of cortistatin and ghrelin systems in the PBMCs of elderly subjects: Putative implications in the postprandial inflammatory response

Gahete, Manuel D.; Luque, Raúl M.; Yubero‐Serrano, Elena M.; Cruz‐Teno, Cristina; Ibáñez‐Costa, Alejandro; Delgado-Lista, Javier; Gracia‐Navarro, Francisco; Pérez-Jiménez, Francisco; Castaño, Justo P.; López‐Miranda, José

doi:10.1002/mnfr.201400059

Cited by 15 publications

(10 citation statements)

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“…RNA extraction, quantification, reverse-transcription as well as the development, validation and application of qPCR to measure the expression levels of different human transcripts have been previously reported elsewhere by our group [ 41 – 45 ]. Total RNA was extracted from paraffin-embedded breast cancer samples, frozen xenografted tumors and cell lines using Trizol (Life Technologies, Barcelona, Spain) following the manufacturer's protocol and subsequently treated with DNase (Promega, Barcelona, Spain).…”

Section: Methodsmentioning

confidence: 99%

The truncated somatostatin receptor sst5TMD4 stimulates the angiogenic process and is associated to lymphatic metastasis and disease-free survival in breast cancer patients

et al. 2016

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The truncated somatostatin receptor sst5TMD4 is associated with poor prognosis in breast cancer and increases breast cancer cell malignancy. Here, we examined the cellular/molecular mechanisms underlying this association, aiming to identify new molecular tools to improve diagnosis, prognosis or therapy. A gene expression array comparing sst5TMD4 stably-transfected MCF-7 cells and their controls (empty-plasmid) revealed the existence of profound alterations in the expression of genes involved in key tumoral processes, such as cell survival or angiogenesis. Moreover, sst5TMD4-overexpressing MCF-7 and MDA-MB-231 cells demonstrated increased expression/production of pro-angiogenic factors and enhanced capacity to form mammospheres. Consistently, sst5TMD4-expressing MCF-7 cells induced xenografted tumors with higher VEGF levels and elevated number of blood vessels. Importantly, sst5TMD4 was expressed in a subset of breast cancers, where it correlated with angiogenic markers, lymphatic metastasis, and reduced disease-free survival. These results, coupled to our previous data, support a relevant role of sst5TMD4 in the angiogenic process and reinforce the role of sst5TMD4 in breast cancer malignancy and metastatic potential, supporting its possible utility to develop new molecular biomarkers and drug therapies for these tumors.

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Section: Methodsmentioning

confidence: 99%

The truncated somatostatin receptor sst5TMD4 stimulates the angiogenic process and is associated to lymphatic metastasis and disease-free survival in breast cancer patients

et al. 2016

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“…Venous blood from the participants (12 h overnight fast) was collected in tubes containing EDTA during the fat-rich meal test, at 0 and 4 h. PBMCs were isolated as previously described [ 21 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

“…500,000 cells/well from each subject were seeded on ultra-low attachment multi 12-well plates (Corning Costar, Sigma) and treated per duplicate with 10% baseline fasting and postprandial serum derived from control and incident-T2DM patients (specifically, we used serum form individuals that developed T2DM during the first two years of follow-up). After the incubation periods (4 and 24 h), PBMCs were centrifuged and RNA was extracted and isolated using TRI-reagent (Sigma) [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Changes in Splicing Machinery Components Influence, Precede, and Early Predict the Development of Type 2 Diabetes: From the CORDIOPREV Study

et al. 2018

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BackgroundType-2 diabetes mellitus (T2DM) is a major health problem with increasing incidence, which severely impacts cardiovascular disease. Because T2DM is associated with altered gene expression and aberrant splicing, we hypothesized that dysregulations in splicing machinery could precede, contribute to, and predict T2DM development.MethodsA cohort of patients with cardiovascular disease (CORDIOPREV study) and without T2DM at baseline (at the inclusion of the study) was used (n = 215). We determined the expression of selected splicing machinery components in fasting and 4 h-postprandial peripheral blood mononuclear cells (PBMCs, obtained at baseline) from all the patients who developed T2DM during 5-years of follow-up (n = 107 incident-T2DM cases) and 108 randomly selected non-T2DM patients (controls). Serum from incident-T2DM and control patients was used to analyze in vitro the modulation of splicing machinery expression in control PBMCs from an independent cohort of healthy subjects.FindingsExpression of key splicing machinery components (e.g. RNU2, RNU4 or RNU12) from fasting and 4 h-postprandial PBMCs of incident-T2DM patients was markedly altered compared to non-T2DM controls. Moreover, in vitro treatment of healthy individuals PBMCs with serum from incident-T2DM patients (compared to non-T2DM controls) reduced the expression of splicing machinery elements found down-regulated in incident-T2DM patients PBMCs. Finally, fasting/postprandial levels of several splicing machinery components in the PBMCs of CORDIOPREV patients were associated to higher risk of T2DM (Odds Ratio > 4) and could accurately predict (AUC > 0.85) T2DM development.InterpretationOur results reveal the existence of splicing machinery alterations that precede and predict T2DM development in patients with cardiovascular disease.FundISCIII, MINECO, CIBERObn.

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“…The most widely studied functional compounds are polyunsaturated fatty acids. In this line, a large range of studies directly performed in humans have shown the ability of n‐3 polyunsaturated fatty acids (n‐3 PUFAs) to alter transcriptional profile in PBMC showing a less pro‐inflammatory environment and a decrease in the expression of pro‐inflammatory factors (Gahete et al., ; Tsunoda et al., ). Additionally, PBMC have been shown to reflect increased lipolytic capacity (increased expression of genes such as PPARα and CPT1A ), which occurs in different tissues in response to the intake of n‐3 PUFAs (Myhrstad et al., ; Radler et al., ).…”

Section: Pbc As a Surrogate Materials For Key Organs And Tissues In Numentioning

confidence: 99%

Peripheral Blood Cells, a Transcriptomic Tool in Nutrigenomic and Obesity Studies: Current State of the Art

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et al. 2018

Comp Rev Food Sci Food Safe

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Gene expression profile of peripheral blood cells (PBC) is able to reflect useful aspects of the whole body metabolic status. Therefore, and favored by the huge development of "omic" technologies, blood cells and, particularly, the peripheral blood mononuclear cell (PBMC) fraction, are emerging as a potent source of transcriptomic biomarkers of health and disease. In this review we describe and discuss the available information concerning the use of the PBC and the PBMC fraction as a crucial tool for nutrigenomic studies. Results of these studies reveal, as these cells are good indicators of metabolic adaptations to diet and, moreover, as they allow us to monitor from early stages on, the metabolic alterations associated with dietary imbalances. In this way, blood cells present the capacity of reflecting higher risks of suffering from diet-related pathologies, such as obesity and its medical complications. What is more, different studies also show how PBMC are able to evidence the metabolic recovery associated with weight loss or dietary interventions. Besides, recent research points to the utility of ex vivo systems of blood cells to test the efficacy of food bioactives. All in all, PBC constitutes an easily obtainable source of predictive biomarkers of metabolic imbalance and disease related to diet and obesity, and also of metabolic recovery, which appears as highly relevant for developing nutritional preventive strategies in dietetics. Moreover, they could serve to perform relatively simple and economic in vitro tests to assess food bioactive compounds, promoting in this way functional food research and related industry developments.

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Dietary fat alters the expression of cortistatin and ghrelin systems in the PBMCs of elderly subjects: Putative implications in the postprandial inflammatory response

Abstract: Our results suggest a potential relevant role of CORT/ssts and ghrelin systems in regulating PBMCs response to nutrient intake, which could help to explain the positive effects of n-3 PUFAs-enriched diets in reducing the inflammatory response.

Cited by 15 publications

References 67 publications

The truncated somatostatin receptor sst5TMD4 stimulates the angiogenic process and is associated to lymphatic metastasis and disease-free survival in breast cancer patients

The truncated somatostatin receptor sst5TMD4 stimulates the angiogenic process and is associated to lymphatic metastasis and disease-free survival in breast cancer patients

Changes in Splicing Machinery Components Influence, Precede, and Early Predict the Development of Type 2 Diabetes: From the CORDIOPREV Study

Peripheral Blood Cells, a Transcriptomic Tool in Nutrigenomic and Obesity Studies: Current State of the Art

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