Dietary Fats Differentially Modulate the Expression of Lecithin:Cholesterol Acyltransferase, Apoprotein-A1 and Scavenger Receptor B1 in Rats

Hatahet, Wael; Cole, Lisa A.; Kudchodkar, Bhalchandra J.; Fungwe, Thomas V.

doi:10.1093/jn/133.3.689

Cited by 30 publications

(19 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This particular observation is in line with that of Machado et al [5], who have reported less weight gain in mice fed a TFA diet than in those on a PUFA diet. Furthermore, in the present study, TFA- and n–3 PUFA-fed rats had significantly lower plasma TC and HDL-C levels while MUFA- and n–6 PUFA-fed rats had significantly higher plasma TC and HDL-C levels, which is in line with the observation of Hatahet et al [14]. However, the proportion of HDL-C in TC was not altered by any of these dietary treatments.…”

Section: Discussionsupporting

confidence: 92%

Chronic Consumption of <i>trans-</i>Fat-Rich Diet Increases Hepatic Cholesterol Levels and Impairs Muscle Insulin Sensitivity without Leading to Hepatic Steatosis and Hypertriglyceridemia in Female Fischer Rats

Jeyakumar¹,

Prashant²,

Rani³

et al. 2011

Ann Nutr Metab

View full text Add to dashboard Cite

Background: The impact of industrial trans fatty acids (TFAs) on lipid metabolism and health remains elusive. Methods: We compared the effect of long-term (52 weeks) ingestion of 10% partially hydrogenated vegetable oil, providing 4.2% of total energy from TFAs, on hepatic lipid metabolism and muscle insulin sensitivity in weanling female Fischer rats with that of palmolein (monounsaturated fatty acid, MUFA), sunflower (n–6 polyunsaturated fatty acid, PUFA), and a blend of sunflower and fish oil (n–3 PUFA). Results: The proportion of plasma high-density lipoprotein cholesterol in total cholesterol and reverse cholesterol transport-associated protein expressions were similar in all the groups. Despite higher lipogenic-pathway protein levels, steatosis or hypertriglyceridemia was not observed in TFA-fed rats. Though TFA ingestion had no effect on fasting plasma glucose, insulin levels or oral glucose tolerance, it significantly decreased muscle insulin-stimulated glucose uptake as compared to PUFAs. Further, TFA ingestion increased adipose tissue retinol-binding protein 4 mRNA as compared to PUFAs (n–6 or n–3). The effects of MUFA (oleic acid) on all these parameters were comparable to those observed for TFAs. Conclusions: Compared to PUFA-rich diets, chronic consumption of a TFA-rich diet did not lead to steatosis or hypertriglyceridemia; however, it significantly impaired muscle insulin sensitivity, while no changes were found in the oral glucose tolerance test.

show abstract

Section: Discussionsupporting

confidence: 92%

Chronic Consumption of <i>trans-</i>Fat-Rich Diet Increases Hepatic Cholesterol Levels and Impairs Muscle Insulin Sensitivity without Leading to Hepatic Steatosis and Hypertriglyceridemia in Female Fischer Rats

Jeyakumar¹,

Prashant²,

Rani³

et al. 2011

Ann Nutr Metab

View full text Add to dashboard Cite

show abstract

“…Feeding hamsters with miristic acid (a SAFA) modulates the SR-BI expression in the liver [34] . An increase in SR-BI expression was observed after feeding rats with oleic acid (a MUFA) [35] or in hamsters fed with omega-6 PUFAs [36] . Recently, an increase in hepatic mRNA levels for SR-BI was observed in mice fed omega-3 PUFAs [37] .…”

Section: Introductionmentioning

confidence: 99%

“…A metabolic effect similar to that reported in SR-BI-overexpressing mice [34] was observed in our study with rats fed the FO-diet ( fi g. 2, 4 a). Studies evaluating a possible regulatory link between dietary fatty acids and hepatic SR-BI expression have been previously reported [34][35][36][37] . Hamsters fed miristic acid [34] , a SAFA, showed a reduction of protein SR-BI expression.…”

mentioning

confidence: 99%

“…Hamsters fed miristic acid [34] , a SAFA, showed a reduction of protein SR-BI expression. Rats fed oleic acid [35] , a MUFA, and hamsters fed omega-6 PUFAs exhibited increased hepatic levels of SR-BI mRNA and protein [36] , while mice fed omega-3 PUFAs showed increased hepatic mRNA expression for SR-BI [37] . These latter fi ndings suggested a potential transcriptional mechanism for the regulation of hepatic SR-BI expression by PUFAs.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Comparative Effect of Fish Oil Feeding and Other Dietary Fatty Acids on Plasma Lipoproteins, Biliary Lipids, and Hepatic Expression of Proteins Involved in Reverse Cholesterol Transport in the Rat

2005

View full text Add to dashboard Cite

Background: While elevated plasma high-density lipoprotein (HDL) levels has been associated to a reduction in cardiovascular risk, dietary fish oils rich in omega–3 polyunsaturated fatty acids (PUFAs) may protect against this disease. The protective effect of HDL is associated to its participation in the reverse cholesterol transport pathway. On the other hand, omega–3 PUFAs decrease plasma HDL levels compared to other fatty acids, which may suggest an effect on reverse cholesterol transport. Aim: In this work, the effect of dietary fish oil on the fatty acid composition of hepatic membranes, plasma lipoprotein cholesterol profile, biliary lipids, and the expression of proteins involved in reverse cholesterol transport, was compared to other dietary oils having a different degree of fatty acid unsaturation. Methods: Male rats were fed a semi synthetic diet containing fish oil (omega–3), sunflower oil (omega–6), olive oil (omega–9) or coconut oil (saturated). Hepatic membrane fatty acid composition, plasma cholesterol levels, lipoprotein cholesterol profile, biliary lipids, hepatic mRNA levels for lecithin cholesterol acyltransferase, hepatic lipase, apo E, and apo A-I, and hepatic protein levels of the scavenger receptor class B type I, caveolin-1, and the ATP binding cassette transporter A1 were analyzed. Plasma apo A-I and apo E protein levels were also evaluated. Results: Compared to the other diets, omega–3 PUFAs significantly changed omega–3/omega–6 fatty acid ratio of hepatic membranes, caused a reduction of plasma total and HDL cholesterol, and selectively increased biliary cholesterol secretion. No modification in the expression levels of lecithin cholesterol acyltransferase, hepatic lipase, apo A-I and apo E mRNA was observed. Hepatic scavenger receptor class B type I, caveolin-1, and the ATP binding cassette transporter A1 protein levels were also not affected. Plasma apo A-I, but not apo E, was reduced. Conclusions: These results show that dietary omega–3 PUFAs reduce plasma HDL cholesterol and increase biliary cholesterol without concomitant modifications in the expression of key genes and proteins involved in reverse cholesterol transport. These findings suggest that functional changes in the activity of these proteins as consequence of the incorporation of omega-3 PUFAs into hepatic membranes and plasma lipoproteins may underlie the effect of fish oil feeding on plasma and hepatic cholesterol metabolism in the rat.

show abstract

“…Este efecto se ratifica en nuestro estudio, que muestra un significativo aumento del HDL-colesterol de un 97% en el grupo tratado. Ello se podría explicar porque, en respuesta a una dieta alta en grasas, se genera una probable disminución en la captación hepática de HDL por una regulación negativa del receptor de HDL (Scavenger Receptor B1 -SRB1), dificultando el transporte reverso de colesterol 33 . Adicionalmente, dietas bajas en grasas a largo plazo han mostrado un incremento en los niveles plasmáticos de triglicéridos 29 .…”

Section: Discussionunclassified

Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol

et al. 2017

View full text Add to dashboard Cite

Background: It is known that some nutrients play an important role in the development of cholelithiasis. Cholesterol is carried by micelles and vesicles inL a litiasis biliar es considerada una patología metabólica multifactorial, siendo la dieta un factor importante en el desarrollo de esta patología. Al respecto, existen antecedentes que sugieren que una dieta alta en grasas es un probable factor predisponente para el desarrollo de litiasis biliar. Sin embargo, aun no se ha dilucidado el impacto que ejercen dietas altas en grasas en los lípidos biliares, debido a diferencias en el metabolismo lipídico inherentes al huésped, en el momento de la intervención dietética. Se han realizado estudios metabólicos en sujetos con perfil lipídico en el rango normal e hiperlipidémicos, con y sin cálculos vesiculares, pero sin lograr obtener un patrón consistente 1,2 . Inicialmente se postuló, que la adición de grasas en dietas destinadas a reducir el peso corporal, podrían disminuir la incidencia de colelitiasis, al potenciar la contractibilidad de la vesícula biliar 3 . Sin embargo, se han reportado resultados contradictorios, indicando que la elevada contractibilidad vesicular por alta ingesta de grasas, no es suficiente explicación para la coleli- Se ha logrado establecer, que una dieta excesiva en colesterol, es un factor predisponente a hiperlipidemia, y puede inducir una sobresaturación de la bilis y formación de cálculos vesiculares. Sin embargo, el tipo de dieta grasa, y específicamente ciertos ácidos grasos, pueden también influenciar la colelitiasis [5][6][7][8] . Ensayos realizados en un modelo animal de conejos con hipercolesterolemia, señalan que la ingesta de una dieta alta en ácidos grasos monoinsaturados, logran normalizar los porcentajes de lípidos biliares, reduciendo el índi-ce litogénico; por el contrario, una dieta alta en áci-dos grasos ω-3-poliinsaturados incrementaron el índice litogénico 9 . En hámsteres se ha informado, que la incorporación de ácidos grasos saturados a la dieta incrementan los efectos litogénicos 10,11

show abstract

Dietary Fats Differentially Modulate the Expression of Lecithin:Cholesterol Acyltransferase, Apoprotein-A1 and Scavenger Receptor B1 in Rats

Cited by 30 publications

References 55 publications

Chronic Consumption of <i>trans-</i>Fat-Rich Diet Increases Hepatic Cholesterol Levels and Impairs Muscle Insulin Sensitivity without Leading to Hepatic Steatosis and Hypertriglyceridemia in Female Fischer Rats

Chronic Consumption of <i>trans-</i>Fat-Rich Diet Increases Hepatic Cholesterol Levels and Impairs Muscle Insulin Sensitivity without Leading to Hepatic Steatosis and Hypertriglyceridemia in Female Fischer Rats

Comparative Effect of Fish Oil Feeding and Other Dietary Fatty Acids on Plasma Lipoproteins, Biliary Lipids, and Hepatic Expression of Proteins Involved in Reverse Cholesterol Transport in the Rat

Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol

Contact Info

Product

Resources

About