Dietary influence of kefir on microbial activities in the mouse bowel

Marquina, Domingo; Santos, Andrés de la Oliva; Corpas, Isabel; Muñoz, J.A.; Zazo, J; Peinado, J. M.

doi:10.1046/j.1472-765x.2002.01155.x

Cited by 59 publications

(50 citation statements)

References 21 publications

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“…Lactobacillus can be classified as 'colonising' species [1] , but its amount was markedly decreased in kefir-treated layers herein. The potential of probiotics to improve the beneficial bacteria while possibly suppressing the pathogenic ones in the intestines have been shown previously [8,17,26,27] . Further, an increase of beneficial microorganisms in the intestine affected the performance favourably [28] .…”

Section: Yeni̇ce çElebi̇ Yörük Uçar Sağlam Tunç Altunmentioning

confidence: 99%

“…Kefir is a milk-based product and has long been used as soft drink in Northern Caucasia. It involves various bacteria and yeasts [16][17][18] . It ensures the enhancement and development of beneficial bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…It ensures the enhancement and development of beneficial bacteria. These bacteria inhabit at the intestinal mucosa and provide an easier clearance of pathogen microorganisms [3,5,15,17,19] .…”

Section: Introductionmentioning

confidence: 99%

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Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

Yenice¹,

Çelebi²,

Yörük³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryIn the current study, the effect of kefir upon the performance, intestinal microflora and histopathology of certain organs in laying hens was investigated. Totally, 108 Lohmann Brown layers, aged 24 weeks, were allocated randomly into three groups, as; Group C (control, n=36): no treatment, Group A (n=36): 10 cc, and Group B (n=36): 7.5 cc kefir per litre of water. Animals were fed for 10 weeks with basal diets. Livers showed moderate level of hydropic degeneration, some lipidosis and focal haemorrhages with high amounts of kefir (Group A). Fewer active follicles in the ovarium were also observed in this group. The egg yield was significantly (P<0.01) lower in Group A (89.40±0.91) than in Group C (92.50±0.83) and Group B (92.86±0.87). For the pH of large intestines, unlike the small ones, it was significantly changed (P<0.01) from basic to acidic milieu in kefir-treated groups. The titres of coliform (E. coli), aerobic (Lactobacillus spp.), and anaerobic bacteria (Peptostreptococcus spp.) were significantly decreased (P<0.05 to P<0.001) with increased intake for both intestinal tracts. We conclude that; i) high kefir intake could unfavourably impair the digestive organ structures, ii) the supplementations led to a marked decrease in the large intestinal pH and microbiological load of the intestines, and iii) high kefir level markedly decreased the egg yield, unlike the low concentration as leading to a considerable improvement from the 6 th weeks onwards. Keywords: Kefir, Laying hen, Microbiology, Pathology, Probiotic Yumurtacı Tavuklarda Kefirin Performans, Barsak Mikroflorası ve Bazı Organların Histopatolojisi Üzerine Etkisi ÖzetMevcut araştırmada, yumurtacı tavuklarda kefirin performans, barsak mikroflorası ve bazı organların histopatolojisi üzerine etkisi araştırıldı. 24 haftalık toplam 108 adet Lohmann Brown yumurtacı tavuk; Grup K (kontrol, n=36), Grup A (n=36): 10 cc ve Grup B (n=36): 7.5 cc kefir/L su olarak rastgele 3 gruba ayrıldı. Hayvanlar 10 hafta süreyle bazal rasyonla beslendi. Yüksek kefir miktarı (Grup A), karaciğerlerde orta düzey hidropik dejenerasyon, belli düzeyde lipidosis ve fokal hemorajiler oluşturdu. Ayrıca, bu grupta ovaryumdaki aktif follikül sayısının daha az olduğu gözlendi. Grup A'daki yumurta verimi (89.40±0.91), Grup C (92.50±0.83) ve Grup B'dekinden (92.86±0.87) önemli düzeyde (P<0.01) daha düşük bulundu. Kefir uygulanan gruplardaki kalın bağırsak pH'sı, ince bağırsakların aksine, bazikten asidik ortama doğru önemli düzeyde (P<0.01) değişti. Koliform (E. coli), aerobik (Lactobacillus spp.), ve anaerobik bakteri (Peptostreptococcus spp.) titreleri artan probiyotik alımıyla birlikte önemli düzeyde (P<0.05 -P<0.001) azaldı. Sonuç olarak; i) yüksek kefir alımının sindirim organı yapılarını olumsuz yönde etkileyebildiği, ii) katkıların kalın bağırsak pH'sını ve bağırsakların mikrobiyolojik yükünü önemli düzeyde azalttığı, ve iii) yüksek kefir düzeyinin yumurta verimini önemli düzeyde azaltmasına karşın, düşük konsantrasyonun 6. haftadan sonra belli oranda artırdığı kanısına va...

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Section: Yeni̇ce çElebi̇ Yörük Uçar Sağlam Tunç Altunmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

Yenice¹,

Çelebi²,

Yörük³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…This hypothesis is supported by the fact that Lactobacillus strains were found in kefir suspension formulation (Table 1), to small cauliflower branches, with a diameter of 0.3-3.5 cm, and is composed of about 890-900 g. , 1988;KOROLEVA, 1988;ÂNGULO;LOPEZ;LEMA, 1993;PINTADO;LOPES;FERNANDES, 1996;REA et al, 1996). Many studies have been carried out on the distribution and constitution of the kefir microbiota (PIDOUX et al, 1990;ÂNGULO;LOPEZ;LEMA, 1993;OHARA et al, 1997;WATABE et al, 1998;MITSUE;TACHIBANA;FUJI, 1999;GARROTE;ANTONINI, 2001;MARQUINA, 2002;SIMOVA et al, 2002). Bottazzi and Bianchi (1980) suggested that the populations found in the kefir grains are not randomly distributed in the grains; Lactobacillus, for example, is found in the grain periphery.…”

Section: Resultsmentioning

confidence: 99%

Microbial profile of a kefir sample preparations: grains in natura and lyophilized and fermented suspension

Bergmann

Pereira

Veiga

et al. 2010

Ciênc. Tecnol. Aliment.

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“…3,11 Kefir also seems to be a promising compound in terms of the enhancement of mucosal resistance to gastrointestinal pathogen infection. 12,13 In addition, kefir was found to have antiallergic and antimutagenic effects. 8,12,14 Kefir as a complex fermented milk, together with its cell-free fraction (KF), was able to exert beneficial effects on the immune system and prevent several types of cancer: 15 Ehrlich carcinoma or Sarcoma 180, Lewis lung carcinoma, E-ascites carcinoma (EC), human mammary cancer (MCF-7), and human T-lymphotropic virus-(HTLV)-positive malignant T-lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

201 Kefir Induces Cell-Cycle Arrest and Apoptosis in HTLV-1 Negative Malignant T-Lymphocytes

Rizk

Maalouf

Baydoun

2011

Clinical Lymphoma Myeloma and Leukemia

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Background: Adult lymphoblastic leukemia (ALL) is a malignancy that occurs in white blood cells. The overall cure rate in children is 85%, whereas it is only 40% in adults. Kefir is an important probiotic that contains many bioactive ingredients, which give it unique health benefits. It has been shown to control several cellular types of cancer. Purpose: The present study investigates the effect of a cell-free fraction of kefir on CEM and Jurkat cells, which are human T-lymphotropic virus type I (HTLV-1)-negative malignant T-lymphocytes. Methods: Cells were incubated with different kefir concentrations. The cytotoxicity of the compound was evaluated by determining the percentage viability of cells. The effect of all the noncytotoxic concentrations of kefir on the proliferation of CEM and Jurkat cells was then assessed. The levels of transforming growth factor-alpha (TGF-α), transforming growth factor-beta1 (TGF-β1), matrix metalloproteinase-2 (MMP-2), and MMP-9 mRNA upon kefir treatment were then analyzed using reverse transcriptase polymerase chain reaction (RT-PCR). Finally, the growth inhibitory effects of kefir on cell-cycle progression/apoptosis were assessed by Cell Death Detection (ELISA) and flow cytometry. Results:The maximum cytotoxicity recorded after 48-hours treatment with 80 µg/µL kefir was only 42% and 39% in CEM and Jurkat cells, respectively. The percent reduction in proliferation was very significant, and was dose-, and time-dependent. In both cell lines, kefir exhibited its antiproliferative effect by downregulating TGF-α and upregulating TGF-β1 mRNA expression. Upon kefir treatment, a marked increase in cell-cycle distribution was noted in the preG 1 phase of CEM and Jurkat cells, indicating the proapoptotic effect of kefir, which was further confirmed by Cell Death Detection ELISA. However, kefir did not affect the mRNA expression of metalloproteinases needed for the invasion of leukemic cell lines. Conclusion: In conclusion, kefir is effective in inhibiting proliferation and inducing apoptosis of HTLV-1-negative malignant T-lymphocytes. Therefore, further in vivo investigation is highly recommended.

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Dietary influence of kefir on microbial activities in the mouse bowel

Cited by 59 publications

References 21 publications

Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

Microbial profile of a kefir sample preparations: grains in natura and lyophilized and fermented suspension

201 Kefir Induces Cell-Cycle Arrest and Apoptosis in HTLV-1 Negative Malignant T-Lymphocytes

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