Dietary influences over proliferating cell nuclear antigen expression in the locust midgut

Zudaire, Enrique; Simpson, Stephen J.; Illa, Isabel; Montuenga, Luis M.

doi:10.1242/jeb.01004

Cited by 21 publications

(14 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of our single BrdU administration experiments show that, immediately after injection, all the BrdU-labelled cells in the locust midgut are exclusively located in the niche area. Our previous data confirm these results as positive cells for PCNA (proliferating cell nuclear antigen), which are exclusively localized in the niche area and colocalize with BrdUlabelled cells (Zudaire et al, 2004). When the stem cell of the intestinal villus crypt divides asymmetrically (Bach et al, 2000;Watt and Hogan, 2000), it produces a stem cell and a committed daughter cell that enters into a dividing transient state.…”

Section: Discussionsupporting

confidence: 79%

“…These data suggest that not all the labeled cells that incorporate BrdU (and thus are synthesizing DNA at Day 0) enter into mitosis. According to previous studies (Zudaire et al, 2004), differentiated columnar cells have multiple DNA copies in their nuclei. To produce polyploid or polytenic cells, the cell must undergo multiple S phases without entering into mitosis, a process termed endoreduplication (Edgar and Orr-Weaver, 2001).…”

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

The regenerative nidi of the locust midgut as a model to study epithelial cell differentiation from stem cells

Illa-Bochaca

Montuenga

2006

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARY A better knowledge of the regulatory mechanisms involved in stem cell proliferation and/or differentiation could reveal new methods for the treatment of some diseases. Most previous studies in the field of stem cell biology have been carried out on cultured isolated cells. In the case of adult tissue stem cells, mesenchymal bone marrow derived cells have been most widely studied, while the undifferentiated stem cells present in the epithelial tissues are less known. In order to advance further our understanding of epithelial tissue stem cells, new in vivo models are required. The present study focuses on the dynamics of a new and simple model of intestinal epithelial regeneration found in the midgut of the migratory locust, Locusta migratoria (Linnaeus 1758). The locust midgut consists of three cell types: columnar cells, endocrine cells and undifferentiated regenerative clustered cells. The undifferentiated epithelial midgut cells give rise to two other cell types and are located in a nest of regenerative cells known as regenerative niche. We have performed single and continuous bromodeoxyuridine (BrdU) administration experiments to study regeneration niches and their cellular dynamics. Immunocytochemistry and immunofluorescence techniques were used to detect the incorporation of BrdU into regenerative niches and the presence of FMRFamide-like immunoreactivity, as a marker for endocrine cell differentiation. Some isolated label retaining cells (LRC) were observed at the niche base 10-15 days after the final BrdU administration. We propose that these cells are the stem cells of this epithelial tissue. We also calculated the length of the cell cycle phases for a subpopulation of transit amplifying cells within the regenerative niche: G1, 2.5±0.5 h; S,5.5±0.5 h; G2, 0.75±0.25 h; M, 2.5±0.5 h. These amplifying cells will give rise to the columnar epithelial non-endocrine lineage. The differentiation of an endocrine cell from a niche stem cell occurs less frequently and thus leads to a lower proportion of endocrine cells as compared with epithelial columnar digestive cells (up to three endocrine cells per niche). Endocrine cell commitment seems to occur very early in the differentiation process within the niche, as double-labelled BrdU and FMRF endocrine cells have never been found. The only exception is the endocrine cells located in the ampullar region of the midgut, some of which show double immunostaining after long-term chronic BrdU injection. In summary, we have characterized a new and simple animal model of epithelial stem cell regeneration that may be useful for understanding the complex biological process that drives tissue renewal from undifferentiated and uncommitted progenitor cells.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 87%

The regenerative nidi of the locust midgut as a model to study epithelial cell differentiation from stem cells

Illa-Bochaca

Montuenga

2006

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…These cells have processes that make contact with the GIT contents, the haemolymph and the primary urine, and release FMRFamides as a function of stage in the feeding cycle and the nutritional quality (protein and carbohydrate ratio and concentration) of the food (Zudaire et al 1998). Dietary quality also affects DNA synthesis and may thus impact structural as well as enzymatic changes in the GIT with diet composition (Zudaire et al 2004). Structural remodelling of the GIT over longer time scales (e.g.…”

Section: Discussionmentioning

confidence: 99%

The gastrointestinal tract as a nutrient-balancing organ

et al. 2010

View full text Add to dashboard Cite

Failure to provision tissues with an appropriate balance of nutrients engenders fitness costs. Maintaining nutrient balance can be achieved by adjusting the selection and consumption of foods, but this may not be possible when the nutritional environment is limiting. Under such circumstances, rebalancing of an imbalanced nutrient intake requires post-ingestive mechanisms. The first stage at which such postingestive rebalancing might occur is within the gastrointestinal tract (GIT), by differential release of digestive enzymes-releasing less of those enzymes for nutrients present in excess while maintaining or boosting levels of enzymes for nutrients in deficit. Here, we use an insect herbivore, the locust, to show for the first time that such compensatory responses occur within the GIT. Furthermore, we show that differential release of proteases and carbohydrases in response to nutritional state translate into differential extraction of macronutrients from host plants. The prevailing view is that physiological and structural plasticity in the GIT serves to maximize the rate of nutrient gain in relation to costs of maintaining the GIT; our findings show that GIT plasticity is integral to the maintenance of nutrient balance.

show abstract

“…Control sections did not receive the anti-PCNA monoclonal antibody solution. Clone PC-10 was used according the manufacturer's instructions, as with PCNA detection in fish and insect tissues (Ortega et al 1994;Zudaire et al 2004). Slides were incubated with streptavidin-peroxidase for 10 min and rinsed with PBS.…”

Section: Pcna Immunohistochemical Stainingmentioning

confidence: 99%

Cell Proliferation Detected with Flow Cytometric Cell Cycle Analysis and Immunohistochemical Detection of Proliferating Cell Nuclear Antigen (PCNA) from Somatic Tissues of Eastern Oysters,Crassostrea virginica∗

Jenkins

LaPeyre

2006

Environmental Bioindicators

View full text Add to dashboard Cite

Two novel biomarker of response assays were developed and compared for use with bivalves. Bivalve mollusks are often used as bioindicators to monitor contaminant body burdens and are employed globally in pollution monitoring and as sentinels of environmental quality. The prevalence of proliferating cells in tissues of the eastern oyster, Crassostrea virginica, (n = 10) was investigated by using immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and by flow cytometric analysis of DNA in cell cycle phases. The PCNA protein is highly conserved and central to DNA synthesis in dividing cells. This protein was detected by using a commercial antibody in the histological staining of gills, mantle, digestive gland, stomach, and labial palps. Cells obtained from these same organs and from the hearts of the oysters were used for flow cytometry (FCM). The proportions of nuclei in the S plus G2/M and G2/M fractions were compared with percentages of PCNA-positive cells scored through microscopy. Proliferation responses generated by microscopy and FCM (S plus G2/M) were equal for digestive gland, gills, stomach, and mantle. The proliferation response in labial palps was significantly higher by PCNA microscopy than by DNA FCM (S plus G2/M). For PCNA microscopy results, a significantly higher proliferation response was noted both for labial palps as compared to gills and mantle and for stomach and digestive gland as compared to gills (P < 0.005). For DNA FCM results, the percentages of proliferating cells in G2M fractions were significantly higher for labial palps and heart when compared to gills, mantle, and digestive gland. By FCM (S plus G2M), the percentages of proliferating cells followed a similar trend, although no significant differences were found between organs. The choice of a relatively highly proliferative tissue, such as labial palps as determined by this study, has utility for developing a cell line, for examining proliferation as a biomarker due to stressor impacts in feral oysters, and for detecting impacts of compounds on proliferative mechanisms.

show abstract

Dietary influences over proliferating cell nuclear antigen expression in the locust midgut

Cited by 21 publications

References 49 publications

The regenerative nidi of the locust midgut as a model to study epithelial cell differentiation from stem cells

The regenerative nidi of the locust midgut as a model to study epithelial cell differentiation from stem cells

The gastrointestinal tract as a nutrient-balancing organ

Cell Proliferation Detected with Flow Cytometric Cell Cycle Analysis and Immunohistochemical Detection of Proliferating Cell Nuclear Antigen (PCNA) from Somatic Tissues of Eastern Oysters,Crassostrea virginica∗

Contact Info

Product

Resources

About