Dietary L-carnitine supplementation for heat stressed juvenile pearl gentian grouper(Epinephelusllanolatus (Epinephelus♂×Epinephelusfuscoguttatus♀): Effects on the antioxidant enzyme, survival, and gene expression

AlatwinusaYohana, Mpwaga; Gyan, Watson Ray; Yang, Qihui; Tan, Beiping; Lin, Huaxing; Yi, Yuanming; Chi, Shuyan

doi:10.1016/j.aqrep.2023.101524

Cited by 5 publications

(2 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is a precursor for steroid hormones required for molting and reproduction (Kumar et al, 2018). Additionally, cholesterol gives cell membranes more structural integrity against environmental stressors like heat or salt (Alatwinusa Yohana et al, 2023). It is also a crucial component of lipoproteins, which help animals absorb and transport lipids throughout their bodies.…”

Section: The Kegg Pathway Enrichment Analysis Of Differential Metabol...mentioning

confidence: 99%

Effect of soybean meal replacement with corn gluten meal on the survival, biochemical and metabolic responses, and disease resistance of Pacific white shrimp (Litopenaeus vannamei)

Yohana,

Ray,

Yang

et al. 2024

Annals of Animal Science

Self Cite

View full text Add to dashboard Cite

This study delves into the potential of replacing soybean meal (SBM) with corn gluten meal (CGM) in the diet of Litopaeneaus vannamei. We aimed to investigate the effects of a dietary soybean meal replacement on various aspects of shrimp, including survival, biochemical indices, digestive enzymes, metabolomics, and disease resistance against Vibrio harveyi . To conduct the study, we fed 840 juvenile Pacific shrimp seven different diets, each containing varying levels of CGM. The control group received a diet with 0% CGM; the other diets contained 3%, 5%, 7%, 9%, 12%, and 14% CGM. Each group comprised three replicates, and the shrimp were fed their diets for eight weeks. Our findings revealed that the survival rate of the shrimp ranged from 90.83% to 97.50% and did not differ significantly between the control and those fed diet treatments 2-5. Additionally, there were no significant differences in crude protein, moisture, and lipid content. However, we observed that total antioxidant capacity content increased with the dietary inclusion of CGM. Furthermore, malondialdehyde content decreased with increasing CGM levels, while superoxide dismutase increased, indicating no obvious oxidative damage was observed in CGM treatment groups. Interestingly, shrimp fed diets 2 and 3 had considerably lower alanine aminotransferase activity than shrimp in the control group. The disease resistance in shrimp was improved across all treatment groups, with a notably higher CGM inclusion having the highest level of mortality during the challenge test. Finally, we analyzed the metabolomics data and found PCA score plots clearly separated the control group and shrimp-fed CGM diets. Our study revealed that a 3% substitution rate of SBM with CGM can enhance survival and immunity, regulate metabolites and improve disease resistance.

show abstract

Section: The Kegg Pathway Enrichment Analysis Of Differential Metabol...mentioning

confidence: 99%

Effect of soybean meal replacement with corn gluten meal on the survival, biochemical and metabolic responses, and disease resistance of Pacific white shrimp (Litopenaeus vannamei)

Yohana,

Ray,

Yang

et al. 2024

Annals of Animal Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…MDA serves as the end product of lipid peroxidation(Zhu et al, 2021). Consequently, measuring MDA content can be a valuable biomarker to assess the extent of oxidative damage in animals: AlatwinusaYohana et al, 2023. A decline in MDA concentration points to improved oxidative conditions(Hoseini et al, 2023).…”

mentioning

confidence: 99%

Response of gut microbiota, antioxidation, and disease resistance to Pacific shrimp fed distiller’s dried grains with solubles replaced soybean meal

Yohana,

Ray,

Qihui

et al. 2024

Aquacult Int

Self Cite

View full text Add to dashboard Cite

This experiment aimed to examine the potential of distillers dried grains soluble (DDGS) meal as a substitute for soybean meal (SBM) in the Paci c shrimp diet. The study aimed to investigate how DDGS affected disease resistance, antoxidation, and gut microbiota. 840 shrimp were given varied diets with varying concentrations of DDGS. D1 (0%), D2 2%, D3 4%, D4 6%, D5 8%, D6 10%, and D7 12% were the DDGS levels. Each group had three replicates of the 56-day experiment. Initially, the weight was 0.19 ± 0.00 g. The survival rate was consistently 100% for shrimp-fed DDGS, except for group 7 (14%) and the control group, where shrimp mortality was observed. The total protein (TP) content was improved as the inclusion level of DDGS increased. The glucose (GLU) exhibited a downward trend with higher DDGS inclusion.Similarly, the TG and CHO levels in the serum decreased as the DDGS levels increased. The activities of ALT (alanine transaminase) and AST (aspartate aminotransferase) decreased as the dietary DDGS increased, with the control group showing signi cantly higher ALT and AST activities than those fed DDGS diets (P < 0.05). Furthermore, the SOD (superoxide dismutase) activity signi cantly increased with higher DDGS levels, while the MDA (malondialdehyde) activity showed the opposite trend. Notably, the control exhibited the highest MDA activity value. The most prevalent phylu found in the gut microbiota were Proteobacteria, and Bacteroidetes. Upon completion of a 7-day bacteria challenge, it was observed that shrimp fed with the control diet had a lower mortality rate compared to those fed the DDGS diet. Based on the parameters measured in this study, it was determined that replacing 10% of SBM with DDGS in the feed of Paci c shrimp is feasible. The calculation for shrimp survivalSurvival rate % = [100× End number of shrimp/ Initial number of shrimp] Serum biochemical analysisThis study set out to look into the antioxidant activity and serum biochemical parameters of Paci c shrimp. The levels of TG, CHO, GLU, TP, ALT, AST, MDA, and SOD were among the many activities that our team examined. We used assay kits from the prestigious Nanjing Jiancheng Bioengineering Institute in China to guarantee precise and trustworthy results. We used commercial assay kits from the Nanjing Jiancheng Institute in Nanjing, China, to measure ALT and AST levels. The biuret method was used to determine TP levels. SOD activity was measured using the nitro blue tetrazolium reaction, with the color extracted into butanol and measured at 550 nm. The TBA (thiobarbituric acid) test was used to measure the amount of MDA in the hepatopancreas. Lipid hydroperoxide breakdown was also measured using TBA, resulting in red chemicals at the 532 nm absorbance peak. The analysis of digestive enzymeTo conduct a thorough analysis, we studied how dietary DDGS affects the activity of digestive enzymes, speci cally amylase, lysine, and protease, in shrimp. We selected three shrimp for each group and carefully removed and dissected their intestines. The intestine...

show abstract

Protective effects of butyric acid during heat stress on the survival, immune response, histopathology, and gene expression in the hepatopancreas of juvenile pacific shrimp (L. Vannamei)

Yohana,

Ray,

Yang

et al. 2024

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Dietary L-carnitine supplementation for heat stressed juvenile pearl gentian grouper(Epinephelusllanolatus (Epinephelus♂×Epinephelusfuscoguttatus♀): Effects on the antioxidant enzyme, survival, and gene expression

Cited by 5 publications

References 56 publications

Effect of soybean meal replacement with corn gluten meal on the survival, biochemical and metabolic responses, and disease resistance of Pacific white shrimp (Litopenaeus vannamei)

Effect of soybean meal replacement with corn gluten meal on the survival, biochemical and metabolic responses, and disease resistance of Pacific white shrimp (Litopenaeus vannamei)

Response of gut microbiota, antioxidation, and disease resistance to Pacific shrimp fed distiller’s dried grains with solubles replaced soybean meal

Protective effects of butyric acid during heat stress on the survival, immune response, histopathology, and gene expression in the hepatopancreas of juvenile pacific shrimp (L. Vannamei)

Contact Info

Product

Resources

About