Dietary Lactobacillus plantarum affected on some immune parameters, air-exposure stress response, intestinal microbiota, digestive enzyme activity and performance of narrow clawed crayfish (Astacus leptodactylus, Eschscholtz)

Valipour, Alireza; Nedaei, Shiva; Noori, Ahmad; Khanipour, A A; Hoseinifar, Seyed Hossein

doi:10.1016/j.aquaculture.2019.01.064

Cited by 51 publications

(40 citation statements)

References 63 publications

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“…The positive role of Lactobacillus in the gut, health and immune status, and disease resistance of aquatic animals has also been investigated 16,30,42,68,69 . In a most recent study, dietary supplementation of 10 9 CFU/ mL of L. plantarum, a same concentration to the present study significantly increased the haemolymph parameters, enzymatic activity and LAB counts in the gut of narrow clawed crayfish (Astacus leptotylus) 29 . Therefore, addition of L. acidophilus and L. plantarum in diet generated an augmented community of beneficial bacteria for aquatic animals that might be associated with improved health and immune status of marron in this study.…”

Section: Discussionsupporting

confidence: 72%

“…Dietary supplementation of feed additives have significant impacts on growth, immunity and disease resistance of crustaceans 3,7,9,29,[37][38][39][40] . Probiotic in aqua diets, has positive influence on the growth and immune performance of commercially farmed shrimp and crayfish species.…”

Section: Discussionmentioning

confidence: 99%

“…Probiotic in aqua diets, has positive influence on the growth and immune performance of commercially farmed shrimp and crayfish species. Among the probiotic bacteria, Lactobacillus species are widely used for their beneficial role in health and immunity of crayfish, Astacus leptodactylus and C. cainii 29,41 . Present study evaluated the combined effects of two most potent LAB on growth, hemolymph parameters, biochemical compositions of tail muscle, intestine and hepatopancreas structure, gut microbiota and immune genes of freshwater crayfish marron.…”

Section: Discussionmentioning

confidence: 99%

“…One hundred microliter of bacterial broth from each dilution was cultured in MRS agar (Sigma-Aldrich, Germany), incubated for 48 h at 37 °C under anaerobic conditions. Subsequently the dilution containing 10 9 CFU/mL L. acidophilus and L. plantarum counts in culture plate was selected based on results of previous study on crayfish 29 . The ingredients of basal diet was purchased from a commercial feed supplier (Glenn Forest, Perth, Australia).…”

Section: Discussionmentioning

confidence: 99%

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Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

Foysal

Fotedar

Siddik

et al. 2020

Sci Rep

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This study aimed to investigate the combined effects of two most potent probiotic bacteria Lactobacillus acidophilus and Lactobacillus plantarum on overall health and immune status of freshwater crayfish, marron under laboratory conditions. A total of 36 marron were distributed into six different tanks and two different feeding groups, control and probiotic-fed group. After acclimation, control group was fed with basal diet while probiotic group was fed 10 9 cfU/mL per kg of bacterial supplemented feed for 60 days. The results showed no significant differences in weight gain, however, probiotic feed significantly enhanced some hemolymph parameters and biochemical composition of tail muscle. Histology data revealed better hepatopancreas health and higher microvilli counts in the marron gut fed probiotic diet. The probiotic bacteria triggered significant shift of microbial communities at different taxa level, mostly those reported as beneficial for crayfish. The probiotic diet also enriched the metabolic functions and genes associated with innate immune response of crayfish. Further correlation analysis revealed significant association of some taxa with increased activity for hemolymph and immune genes. therefore, dietary Lactobacillus supplementation can modulate the overall health and immunity as well as gut microbial composition and interaction network between gut microbiota and immune system in crayfish. Aquaculture has become an important food sector for meeting the overall protein demand for growing population. The global consumption of crustaceans has increased greatly and especially those with live transport abilities are increasingly becoming popular 1-3. Marron (Cherax cainni) is one of the largest freshwater crayfish farmed in Western Australia (WA) that has high nutritive value and widespread consumer preferences 4,5. In addition, long distance live transportation ability of marron further increases its international demand, and thus has become an ideal crayfish species for commercial farming 6,7. However, the production of marron in WA has remained stagnant for a long time 7. Selection of proper diets and maintaining the optimum water quality are the two most crucial factors required for marron farming 5,7. Although no disease outbreaks have yet been reported, the interest and intend of intensification in marron farming can expose marron to possible crayfish pathogens including Vibrio, Aeromonas and Rhodobacter 5,6. In the past, several laboratory based trials have been conducted 3,6-9 , however, finding a suitable diet and identifying the beneficial bacteria that potentially can influence the growth and immune performance of marron has still remained unknown. Towards sustainable development of aquaculture, the use of feed additives including probiotics, prebiotics, synbiotics, parabiotics and phytogenics in crustacean's diet to boost biological indices has gained extensive attention from the researchers and farmers. Probiotics are microorganisms associated with health and immune benefits for the host ...

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

Foysal

Fotedar

Siddik

et al. 2020

Sci Rep

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“…Therefore, applications of beneficial bacteria and modulation of intestinal microbiota to improve health and immune responses have been promising means to enhance growth performance and disease resistance in shellfish aquaculture ( Ringo , 2020 ; Wang et al, 2020 ). For instance, Lactobacillus species as feed additives has been reported to improve immune responses and modulate gut microbiota in marron ( Cherax cainii ) ( Foysal et al, 2020 ), improve immune response and disease resistance in Pacific white shrimp ( Litopenaeus vannamei ) ( Roomiani, Ahmadi & Ghaeni, 2018 ), increase growth performance in giant river prawn ( Macrobrachium rosenbergii ) ( Karthik & Bhavan, 2018 ), and enhance immune-related enzymes in crayfish ( Astacus leptodactylus ) ( Valipour et al, 2019 ). Additionally, shrimp feed supplemented with Bacillus has been shown to improve survival under pathogen exposure in black tiger shrimp ( Rengpipat et al, 2003 ) and Pacific white shrimp ( Cai et al, 2019 ; Li, Tan & Mai, 2009b ; Liu et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Multi-omics analysis to examine microbiota, host gene expression and metabolites in the intestine of black tiger shrimp (Penaeus monodon) with different growth performance

Uengwetwanit

Uawisetwathana

Arayamethakorn

et al. 2020

PeerJ

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Understanding the correlation between shrimp growth and their intestinal bacteria would be necessary to optimize animal’s growth performance. Here, we compared the bacterial profiles along with the shrimp’s gene expression responses and metabolites in the intestines between the Top and the Bottom weight groups. Black tiger shrimp (Penaeus monodon) were collected from the same population and rearing environments. The two weight groups, the Top-weight group with an average weight of 36.82 ± 0.41 g and the Bottom-weight group with an average weight of 17.80 ± 11.81 g, were selected. Intestines were aseptically collected and subjected to microbiota, transcriptomic and metabolomic profile analyses. The weighted-principal coordinates analysis (PCoA) based on UniFrac distances showed similar bacterial profiles between the two groups, suggesting similar relative composition of the overall bacterial community structures. This observed similarity was likely due to the fact that shrimp were from the same genetic background and reared under the same habitat and diets. On the other hand, the unweighted-distance matrix revealed that the bacterial profiles associated in intestines of the Top-weight group were clustered distinctly from those of the Bottom-weight shrimp, suggesting that some unique non-dominant bacterial genera were found associated with either group. The key bacterial members associated to the Top-weight shrimp were mostly from Firmicutes (Brevibacillus and Fusibacter) and Bacteroidetes (Spongiimonas), both of which were found in significantly higher abundance than those of the Bottom-weight shrimp. Transcriptomic profile of shrimp intestines found significant upregulation of genes mostly involved in nutrient metabolisms and energy storage in the Top-weight shrimp. In addition to significantly expressed metabolic-related genes, the Bottom-weight shrimp also showed significant upregulation of stress and immune-related genes, suggesting that these pathways might contribute to different degrees of shrimp growth performance. A non-targeted metabolome analysis from shrimp intestines revealed different metabolic responsive patterns, in which the Top-weight shrimp contained significantly higher levels of short chain fatty acids, lipids and organic compounds than the Bottom-weight shrimp. The identified metabolites included those that were known to be produced by intestinal bacteria such as butyric acid, 4-indolecarbaldehyde and L-3-phenyllactic acid as well as those produced by shrimp such as acyl-carnitines and lysophosphatidylcholine. The functions of these metabolites were related to nutrient absorption and metabolisms. Our findings provide the first report utilizing multi-omics integration approach to investigate microbiota, metabolic and transcriptomics profiles of the host shrimp and their potential roles and relationship to shrimp growth performance.

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Use of Probiotics in Shellfish

Sharifuzzaman

Walpita²,

Hasan³

2022

Probiotics in Aquaculture

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Dietary Lactobacillus plantarum affected on some immune parameters, air-exposure stress response, intestinal microbiota, digestive enzyme activity and performance of narrow clawed crayfish (Astacus leptodactylus, Eschscholtz)

Cited by 51 publications

References 63 publications

Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

Multi-omics analysis to examine microbiota, host gene expression and metabolites in the intestine of black tiger shrimp (Penaeus monodon) with different growth performance

Use of Probiotics in Shellfish

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