Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala

Liang, Hualiang; Mokrani, Amina; Chisomo-Kasiya, Hopeson; Ji, Ke; Ge, Xianping; Ren, Mingchun; Liu, Bo; Xi, Bingwen; Sun, Aijun

doi:10.1007/s10695-018-0594-x

Cited by 34 publications

(13 citation statements)

References 68 publications

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“…In addition, the mRNA level of mtor gene was also increased in the leucine programming group, suggesting the activation of mTOR signaling. The leucine in the diet plays an important role in regulating growth performance, body composition, and mTOR signaling pathways in fish [14,38,39]. With the injection of leucine in the hypothalamus of rainbow trout, the mTOR signaling pathway in central nervous system is activated, thereby regulating the digestion and absorption of nutrients [15].…”

Section: Discussionmentioning

confidence: 99%

Early leucine programming on protein utilization and mTOR signaling by DNA methylation in zebrafish (Danio rerio)

Zhu

Wang

et al. 2020

Nutr Metab (Lond)

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Background: Early nutritional programming affects a series of metabolism, growth and development in mammals. Fish also exhibit the developmental plasticity by early nutritional programming. However, little is known about the effect of early amino acid programming on growth and metabolism. Methods: In the present study, zebrafish (Danio rerio) was used as the experimental animal to study whether early leucine stimulation can programmatically affect the mechanistic target of rapamycin (mTOR) signaling pathway, growth and metabolism in the later life, and to undercover the mechanism of epigenetic regulation. Zebrafish larvas at 3 days post hatching (dph) were raised with 1.0% leucine from 3 to 13 dph during the critical developmental stage, then back to normal water for 70 days (83 dph). Results: The growth performance and crude protein content of zebrafish in the early leucine programming group were increased, and consistent with the activation of the mTOR signaling pathway and the high expression of genes involved in the metabolism of amino acid and glycolipid. Furthermore, we compared the DNA methylation profiles between the control and leucine-stimulated zebrafish, and found that the methylation levels of CG-differentially methylated regions (DMGs) and CHH-DMGs of genes involved in mTOR signaling pathway were different between the two groups. With quantitative PCR analysis, the decreased methylation levels of CG type of Growth factor receptorbound protein 10 (Grb10), eukaryotic translation initiation factor 4E (eIF4E) and mTOR genes of mTOR signaling pathway in the leucine programming group, might contribute to the enhanced gene expression. Conclusions: The early leucine programming could improve the protein synthesis and growth, which might be attributed to the methylation of genes in mTOR pathway and the expression of genes involved in protein synthesis and glycolipid metabolism in zebrafish. These results could be beneficial for better understanding of the epigenetic regulatory mechanism of early nutritional programming.

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Section: Discussionmentioning

confidence: 99%

Early leucine programming on protein utilization and mTOR signaling by DNA methylation in zebrafish (Danio rerio)

Zhu

Wang

et al. 2020

Nutr Metab (Lond)

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“…In addition, the mRNA level of mtor gene was also increased in the leucine programming group, suggesting the activation of mTOR signaling. The leucine in the diet plays an important role in regulating growth performance, body composition, and mTOR signaling pathways in sh [14,38,39]. With the injection of leucine in the hypothalamus of rainbow trout, the mTOR signaling pathway in central nervous system is activated, thereby regulating the digestion and absorption of nutrients [15].…”

Section: Discussionmentioning

confidence: 99%

Early Leucine Programming on Protein Utilization and mTOR Signaling by DNA Methylation in Zebrafish (Danio Rerio)

Zhu

Wang

et al. 2020

Preprint

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Background: Early nutritional programming affects a series of metabolism, growth and development in mammals. Fish also exhibit the developmental plasticity by early nutritional programming. However, little is known about the effect of early amino acid programming on growth and metabolism. Methods: In the present study, zebrafish (Danio rerio) was used as the experimental animal to study whether early leucine stimulation can programmatically affect the mechanistic target of rapamycin (mTOR) signaling pathway, growth and metabolism in the later life, and to undercover the mechanism of epigenetic regulation. Zebrafish larvas at 3 days post hatching (dph) were raised with 1.0% leucine from 3 to 13 dph during the critical developmental stage, then back to normal water for 70 days (83 dph). Results: The growth performance and crude protein content of zebrafish in the early leucine programming group were increased, and consistent with the activation of the mTOR signaling pathway and the high expression of genes involved in the metabolism of amino acid and glycolipid. Furthermore, we compared the DNA methylation profiles between the control and leucine-stimulated zebrafish, and found that the methylation levels of CG-differentially methylated regions (DMGs) and CHH-DMGs of genes involved in mTOR signaling pathway were different between the two groups. With quantitative PCR analysis, the decreased methylation levels of CG type of Growth factor receptor-bound protein 10 (Grb10), eukaryotic translation initiation factor 4E (eIF4E) and mTOR genes of mTOR signaling pathway in the leucine programming group, might contribute to the enhanced gene expression. Conclusions: The early leucine programming could improve the protein synthesis and growth, which might be attributed to the methylation of genes in mTOR pathway and the expression of genes involved in protein synthesis and glycolipid metabolism in zebrafish. These results could be beneficial for better understanding of the epigenetic regulatory mechanism of early nutritional programming.

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“…Plasma total glucose (GLU) was measured using an automatic chemical analyzer, Mindray BS-400 (Shenzhen Mindray Biomedical Electronics Co., Ltd., China) following the description by Ren et al (2017). In addition, an automatic chemiluminescence analyzer, Maglumi 1000, was used to determine plasma insulin levels (Snibe Diagnostic Ltd., China) according to the description of Liang et al (2019).…”

Section: Plasma Parametersmentioning

confidence: 99%

“…The method of western blot analysis was performed following the description by Liang et al (2019). In brief, first, the lysate from the sample was prepared, and then western blot analysis was performed through the following main steps: (1) equal amounts of protein were loaded into the wells of the SDS-PAGE gel, (2) the proteins from the gel were transferred to a PVDF membrane, (3) the membrane was blocked using a blocking buffer, (4) the membrane was incubated with monoclonal antibody to GFP or GADPH diluted 1000× (Bio-Transduction Lab, China), (5) the membrane was washed, (6) the membrane was incubated with the of goat anti-mouse IgG-HRP diluted 1000× (Bio-Transduction Lab, China), (7) the secondary antibody was stained by a BeyoECL Star kit (Beyotime Biotechnology, China) based on the manufacturer's instructions, and (8) the images were acquired using chemiluminescence.…”

Section: Western Blot Analysismentioning

confidence: 99%

Functional Characterization of Facilitative Glucose Transporter 4 With a Delay Responding to Plasma Glucose Level in Blunt Snout Bream (Megalobrama amblycephala)

Liang

Maulu

et al. 2020

Front. Physiol.

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Facilitative glucose transporter 4 (GLUT4) plays a central role in mediating insulin function to increase glucose uptake in glucose metabolism homeostasis. In this study, the function and localization of GLUT4 in blunt snout bream (Megalobrama amblycephala) were first investigated, and then, the response measured as carbohydrate level, was analyzed. The results showed that the cDNA sequence of GLUT4 in blunt snout bream (MaGLUT4, GenBank accession no: MT447093) was 2868 bp in length, and the corresponding mRNA contained a 5-UTR region of 513 bp and a 3-UTR region of 837 bp. MaGLUT4 had an open reading frame of 1518 bp and was encoded by 505 amino acids. Its theoretical isoelectric point and molecular weight was 6.41 and 55.47 kDa, respectively. A comparison of these characteristics with BLASTP results from the NCBI database showed that MaGLUT4 had the highest homology with Cypriniformes fish, with MaGLUT4 and GLUT4 of other Cypriniformes clustered in the phylogenetic tree with other GLUT1-4 amino acid sequences. Compared with the results from the homo_sapiens and mus_musculus data sets, some mutations were observed in the GLUT4 amino acid sequence of these aquatic animals, including an FQQI mutation to FQQL, LL mutation to MM, and TELEY mutation to TELDY. MaGLUT4 was constitutively expressed in the muscle, intestine, and liver, with the highest mRNA level observed in muscle. Furthermore, the predicted tertiary structure and results of immunohistochemical staining showed that MaGLUT4 was a transmembrane protein primarily located in the plasma membrane, where it accounts for 60.9% of the total expressed, according to an analysis of subcellular localization. Blood glucose level peaked within 1 h, and the insulin level peaked at 6 h, while the mRNA and protein levels of GLUT4 showed an upward trend with an increase in feeding time and decreased sharply after 12 h. These results confirmed that MaGLUT4 was mainly distributed in muscles and crosses the cell membrane. The changes in the insulin,

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Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala

Cited by 34 publications

References 68 publications

Early leucine programming on protein utilization and mTOR signaling by DNA methylation in zebrafish (Danio rerio)

Early leucine programming on protein utilization and mTOR signaling by DNA methylation in zebrafish (Danio rerio)

Early Leucine Programming on Protein Utilization and mTOR Signaling by DNA Methylation in Zebrafish (Danio Rerio)

Functional Characterization of Facilitative Glucose Transporter 4 With a Delay Responding to Plasma Glucose Level in Blunt Snout Bream (Megalobrama amblycephala)

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