Dietary lipid levels impact lipoprotein lipase, hormone-sensitive lipase, and fatty acid synthetase gene expression in three tissues of adult GIFT strain of Nile tilapia, Oreochromis niloticus

Jian, Tian; Wu, Fan; Yang, Changgeng; Jiang, Ming; Liu, Wei; Huang, Wen

doi:10.1007/s10695-014-0001-1

Cited by 58 publications

(45 citation statements)

References 71 publications

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“…In the present study, the optimal dietary lipid level for larval GIFT tilapia was 8.56%, according to the secondary curve equation, which is similar to the dietary lipid level requirement of adult GIFT tilapia(7.66–8.79% lipid levels) (Tian et al . ). However, this optimal dietary lipid level for larvae is lower than that for juvenile hybrid tilapia and GIFT tilapia (12% and 9.34% respectively) (Chou & Shiau ; Wang et al .…”

Section: Discussionmentioning

confidence: 97%

“…Mjoun, Rosentrater and Brown () recommended that dietary fat levels should be 8–12% for tilapia weighing up to 25 g and 6–8% for larger fish. Tian, Wu, Yang, Jiang, Liu and Wen () revealed that the optimal dietary lipid level for maximum growth performance of adult Genetically Improved Farmed Tilapia (GIFT) tilapia is 7.66–8.79% and that increasing dietary lipid level contributed to increase tissue and whole‐body lipid levels. Increased culture of GIFT Nile tilapia in China is based on their rapid growth rate, high fillet yield and good disease resistance (Qiang, Yang, Wang, Kpundeh & Xu ).…”

Section: Introductionmentioning

confidence: 99%

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Dietary lipid requirements of larval genetically improved farmed tilapia,Oreochromis niloticus(L.), and effects on growth performance, expression of digestive enzyme genes, and immune response

et al. 2016

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The present study was performed to investigate the effects of dietary lipid level on growth performance, antioxidant defense, and mRNA levels of digestive enzymes and immune genes in larval genetically improved farmed tilapia (Oreochromis niloticus). The results show that larvae fed lipid levels of 4-11.5% had greater weight gain (WG), feed efficiency, protein efficiency ratio and survival than larvae fed the other dietary lipid levels (P < 0.05). No difference whole-body crude protein, ash or moisture contents were found between treatments (P > 0.05) but lipid contents increased as dietary lipid level was increased. The 8% and 11.5% dietary lipid levels had stimulatory effects on trypsin mRNA level compared with those of larvae fed the 15% and 18.5% lipid diets. Intestinal and liver amylase mRNA levels decreased in larvae fed the 18.5% lipid diet, following the decrease in dietary starch. Analysis of the secondary curve equation for WG indicated that the optimal dietary lipid level in larval tilapia was 8.56%. In addition, the 4-11.5% lipid levels improved superoxide dismutase and glutathione peroxidase activities and increased mRNA levels of heat shock protein70, hepcidin antimicrobial peptide-1, and C-type lysozyme in the liver of larvae (P < 0.05). A 192-h Streptococcus iniae challenge study was conducted after the 35-day feeding trial, and the results showed that postchallenge mortality of larvae fed the 11.5% lipid level was the lowest and enhanced the immune activities.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Dietary lipid requirements of larval genetically improved farmed tilapia,Oreochromis niloticus(L.), and effects on growth performance, expression of digestive enzyme genes, and immune response

et al. 2016

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“…Mesenteric adipose tissue is recognized as an important fat storage tissue (Weil et al, ) and substantially expresses lipid metabolic enzymes in fish (Tian, Lu, et al, ; Tian, Wu, et al, ). In this study, the mRNA levels of fas decreased after AICAR treatment, while the mRNA levels of srebp‐1 , hsl and lpl remained unchanged.…”

Section: Discussionmentioning

confidence: 99%

“…The primers used for these genes are shown in Table . All primers were synthesized by BGI and have been used successfully in grass carp in previous studies (Cheng et al, , ; Hu et al, ; Leng et al, ; Sun et al, ; Tian, Lu, et al, ; Tian, Wu, et al, ). The key glucose metabolic enzymes in the liver, muscle and heart were tested (Hemre et al, ; Hu et al, ), and the lipid metabolic factors in the liver, muscle and adipose tissue were detected, respectively (Weil et al, ).…”

Section: Methodsmentioning

confidence: 99%

AMPK activation by dietary AICAR affects the growth performance and glucose and lipid metabolism in juvenile grass carp

et al. 2019

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Fish are poor users of dietary carbohydrates and considered to be glucose intolerant, and activating AMP‐activated protein kinase (AMPK) by 5‐aminoimidazole‐4‐carboxamide ribonucleoside (AICAR) has been shown to stimulate glucose uptake and utilization in skeletal muscle cells of rainbow trout. However, the effects of activating AMPK by dietary AICAR in the piscine model remain largely unexplored. In the present study, grass carp were fed purified diets including AICAR at a level of 0 or 200 mg/kg diet to chronically activate AMPK for 8 weeks, and the dietary effects on phenotypic and molecular responses related to glucose and lipid metabolism were examined. The results showed that activating AMPK by dietary AICAR significantly reduced fish growth performance, serum glucose and triacylglycerol contents, and liver lipid contents, while it increased hepatic glycogen contents (p < 0.05). The survival was not influenced by AICAR treatment (p > 0.05). The results of AMPK signalling pathway in both the liver and muscle show that dietary AICAR increased the phosphorylation of AMPKα and acetyl‐CoA carboxylase (ACC; p < 0.05), while inhibiting the phosphorylation of sterol regulatory element‐binding transcription factor‐1(SREBP‐1) and target of rapamycin (p < 0.05). The activation of AMPK reduced the mRNA expression of lipogenesis enzymes in the liver and muscle (p < 0.05) and increased mRNA expression of gluconeogenesis enzymes in the liver and glycolysis enzymes in the heart (p < 0.05). Our results demonstrate that activating AMPK by AICAR reduces serum glucose and inhibits activities of ACC and SREBP‐1 to reduce lipogenesis in the liver and muscle, and AMPK could be used as an active target for its metabolic syndromes of grass carp.

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“…In dietinduced obese mice, transgenic overexpression adropin exhibit inhibition LPL genes expression in adipose tissue (Kumar et al 2008). Given that the hepatic LPL gene is substantially expressed in fish (Albalat et al 2006, Feng et al 2014, Tian et al 2015, correlations between adropin and LPL were suspected to play a role in lipid metabolism at the tilapia hepatocytes level. This idea is supported by our in vitro studies with tilapia hepatocytes in which adropin was effective in elevating LPL release, cellular LPL content and total LPL production.…”

Section: Discussionmentioning

confidence: 99%

Adropin induction of lipoprotein lipase expression in tilapia hepatocytes

Lian

Liu

et al. 2016

Journal of Molecular Endocrinology

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The peptide hormone adropin plays a role in energy homeostasis. However, biological actions of adropin in non-mammalian species are still lacking. Using tilapia as a model, we examined the role of adropin in lipoprotein lipase (LPL) regulation in hepatocytes. To this end, the structural identity of tilapia adropin was established by 5 0 /3 0 -rapid amplification of cDNA ends (RACE). The transcripts of tilapia adropin were ubiquitously expressed in various tissues with the highest levels in the liver and hypothalamus. The prolonged fasting could elevate tilapia hepatic adropin gene expression, whereas no effect of fasting was observed on hypothalamic adropin gene levels. In primary cultures of tilapia hepatocytes, synthetic adropin was effective in stimulating LPL release, cellular LPL content, and total LPL production. The increase in LPL production also occurred with parallel rises in LPL gene levels. In parallel experiments, adropin could elevate cAMP production and up-regulate protein kinase A (PKA) and PKC activities. Using a pharmacological approach, cAMP/PKA and PLC/inositol trisphosphate (IP3)/PKC cascades were shown to be involved in adropin-stimulated LPL gene expression. Parallel inhibition of p38MAPK and Erk1/2, however, were not effective in these regards. Our findings provide, for the first time, evidence that adropin could stimulate LPL gene expression via direct actions in tilapia hepatocytes through the activation of multiple signaling mechanisms.

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Dietary lipid levels impact lipoprotein lipase, hormone-sensitive lipase, and fatty acid synthetase gene expression in three tissues of adult GIFT strain of Nile tilapia, Oreochromis niloticus

Cited by 58 publications

References 71 publications

Dietary lipid requirements of larval genetically improved farmed tilapia,Oreochromis niloticus(L.), and effects on growth performance, expression of digestive enzyme genes, and immune response

Dietary lipid requirements of larval genetically improved farmed tilapia,Oreochromis niloticus(L.), and effects on growth performance, expression of digestive enzyme genes, and immune response

AMPK activation by dietary AICAR affects the growth performance and glucose and lipid metabolism in juvenile grass carp

Adropin induction of lipoprotein lipase expression in tilapia hepatocytes

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