Dietary Milk-Fat-Globule Membrane Affects Resistance to Diarrheagenic Escherichia coli in Healthy Adults in a Randomized, Placebo-Controlled, Double-Blind Study

Bruggencate, Sandra J Ten; Frederiksen, Pernille; Pedersen, Simon Metz Mariendal; Floris-Vollenbroek, Esther G; Bos, Elly Lucas-van de; Hoffen, Els van; Wejse, Peter Langborg

doi:10.3945/jn.115.214098

Cited by 46 publications

(50 citation statements)

References 35 publications

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“…Novakovic et al found that the isolated individual proteins of porcine MFGM could inhibit F4ac‐positive enterotoxigenic E. coli from attaching to host intestinal epithelium . E.coli challenge studies in healthy adults also demonstrated protective effects of MFGM . Our findings are consistent with these studies, and the antimicrobial constituents of MFGM also contributed to the overall improvement.…”

Section: Discussionsupporting

confidence: 90%

“…From day 2 to day 14 postoperation, both Sham and SBS groups received daily administration of 1mL double-distilled (dd) water by intragastric gavage, while the SBS+MFGM group was gavaged with MFGM (Lacprodan MFGM-10, Arla Foods Ingredients group, Aarhus, Denmark) resolved in 1 mL dd water at a dosage of 1.5g/kg/d. [19][20][21] Body weight and food intake were monitored every day. Stool consistency was recorded and rated on a defined scale where 0 = formed pellets, 2 = loose or semiformed, 4 = watery.…”

Section: Experimental Designmentioning

confidence: 99%

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Milk Fat Globule Membrane Inhibits NLRP3 Inflammasome Activation and Enhances Intestinal Barrier Function in a Rat Model of Short Bowel

Niu

et al. 2018

J Parenter Enteral Nutr

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Section: Discussionsupporting

confidence: 90%

Section: Experimental Designmentioning

confidence: 99%

Milk Fat Globule Membrane Inhibits NLRP3 Inflammasome Activation and Enhances Intestinal Barrier Function in a Rat Model of Short Bowel

Niu

et al. 2018

J Parenter Enteral Nutr

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“…These results are not unexpected as previous authors found that MFGM from raw or heat-treated milk inhibited the expression of the Shiga toxin gene in E. coli O157:H7 [25] while in humans orally challenged with live, attenuated diarrheagenic E. coli the intake of a milk protein concentrate rich in MFGM improved the in vivo resistance to this bacteria [26]. …”

Section: Introductionsupporting

confidence: 58%

Isolation and Analysis of Phospholipids in Dairy Foods

Pimentel

Gomes

Pintado

et al. 2016

Journal of Analytical Methods in Chemistry

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The lipid fraction of milk is one of the most complex matrixes in foodstuffs due to the presence of a high number of moieties with different physical and chemical properties. Glycerolipids include glycerol and two fatty acids esterified in positions sn-1 and sn-2 with higher concentration of unsaturated fatty acids than in the triglyceride fraction of milk. Sphingolipids consist of a sphingoid base linked to a fatty acid across an amide bond. Their amphiphilic nature makes them suitable to be added into a variety of foods and recent investigations show that phospholipids, mainly phosphatidylserine and sphingomyelin, can exert antimicrobial, antiviral, and anticancer activities as well as positive effects in Alzheimer's disease, stress, and memory decline. Polar lipids can be found as natural constituents in the membranes of all living organisms with soybean and eggs as the principal industrial sources, yet they have low contents in phosphatidylserine and sphingomyelin. Animal products are rich sources of these compounds but since there are legal restrictions to avoid transmission of prions, milk and dairy products are gaining interest as alternative sources. This review summarizes the analysis of polar lipids in dairy products including sample preparation (extraction and fractionation/isolation) and analysis by GC or HPLC and the latest research works using ELSD, CAD, and MS detectors.

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“…We recently reported that infants fed the MFGM supplemented formula had a lower incidence of AOM and less use of antipyretics [17], and Bruggencate et al [34] found that MFGM intake reduced Escherichia coli induced diarrhea in adults. Taken together, these results support a modifying effect of MFGM on the proliferation of certain bacterial species and/or symptoms of infection.…”

Section: Discussionmentioning

confidence: 99%

Oral Microbiota in Infants Fed a Formula Supplemented with Bovine Milk Fat Globule Membranes - A Randomized Controlled Trial

et al. 2017

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BackgroundIn a recent study, supplementation of infant formula with milk fat globule membranes (MFGM) decreased the incidence of otitis media in infants <6 months of age.ObjectivesThe aim of the present study was to characterize the oral microbiota in infants fed MFGM-supplemented formula and compare it to that of infants fed standard formula or breast milk.MethodsIn a prospective double-blinded randomized controlled trial, exclusively formula-fed infants <2 months of age were randomized to be fed experimental formula (EF, n = 80) with reduced energy and protein and supplemented with a bovine MFGM concentrate, or standard formula (SF, n = 80) until 6 months of age. A breast-fed reference (BFR, n = 80) group was also recruited. The oral microbiota was analyzed at 4 (n = 124) and 12 (n = 166) months of age using Illumina MiSeq multiplex sequencing and taxonomic resolution against the HOMD 16S rDNA database of oral bacteria.ResultsSpecies richness in the oral samples did not differ between the EF and SF groups, but partial least square modeling identified a few taxa that were significantly associated with being in either group, e.g. lower level of Moraxella catarrhalis in the EF group. Infants in the BFR group had significantly lower species richness at 4 months of age and their microbiota pattern differed markedly from the formula-fed groups.ConclusionsSupplementation of infant formula with MFGM yielded moderate effects on the oral microbiome. Moraxella catarrhalis was less prevalent in infants fed EF than in those fed SF and may be associated with the decrease in otitis media seen in the same group.

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Dietary Milk-Fat-Globule Membrane Affects Resistance to Diarrheagenic Escherichia coli in Healthy Adults in a Randomized, Placebo-Controlled, Double-Blind Study

Cited by 46 publications

References 35 publications

Milk Fat Globule Membrane Inhibits NLRP3 Inflammasome Activation and Enhances Intestinal Barrier Function in a Rat Model of Short Bowel

Milk Fat Globule Membrane Inhibits NLRP3 Inflammasome Activation and Enhances Intestinal Barrier Function in a Rat Model of Short Bowel

Isolation and Analysis of Phospholipids in Dairy Foods

Oral Microbiota in Infants Fed a Formula Supplemented with Bovine Milk Fat Globule Membranes - A Randomized Controlled Trial

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