Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon

Gurwara, Shawn; Ajami, Nadim J.; Jang, Albert; Hessel, Frances C.; Chen, Liang; Plew, Sarah; Wang, Zhensheng; Graham, David Y.; Hair, Clark; White, Donna L.; Kramer, Jennifer R.; Kourkoumpetis, Themistoklis; Hoffman, Kristi L.; Cole, Rhonda A.; Hou, Jason K.; Husain, Nisreen; Jarbrink-Sehgal, Maria; Hernáez, Rubén; Kanwal, Fasiha; Ketwaroo, Gyanprakash A.; Shah, Rajesh; Velez, Maria; Natarajan, Yamini; El–Serag, Hashem B.; Petrosino, Joseph F.; Jiao, Li

doi:10.3390/nu11030613

Cited by 57 publications

(46 citation statements)

References 47 publications

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“…Especially for water-soluble vitamins (e.g., vitamin B family and vitamin C), it is important to consume a diet containing the necessary amounts of these vitamins. There was a significantly higher vitamin B9 intake for subjects in C1 and C3 compared to C2 or C4 ( Table 3 ) supporting the research of bacterial richness, and composition differed significantly by the consumption of folate and B vitamin group [ 54 ].…”

Section: Discussionsupporting

confidence: 67%

Obesity Measures and Dietary Parameters as Predictors of Gut Microbiota Phyla in Healthy Individuals

et al. 2020

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The dynamics and diversity of human gut microbiota that can remarkably influence the wellbeing and health of the host are constantly changing through the host’s lifetime in response to various factors. The aim of the present study was to determine a set of parameters that could have a major impact on classifying subjects into a single cluster regarding gut bacteria composition. Therefore, a set of demographical, environmental, and clinical data of healthy adults aged 25–50 years (117 female and 83 men) was collected. Fecal microbiota composition was characterized using Illumina MiSeq 16S rRNA gene amplicon sequencing. Hierarchical clustering was performed to analyze the microbiota data set, and a supervised machine learning model (SVM; Support Vector Machines) was applied for classification. Seventy variables from collected data were included in machine learning analysis. The agglomerative clustering algorithm suggested the presence of four distinct community types of most abundant bacterial phyla. Each cluster harbored a statistically significant different proportion of bacterial phyla. Regarding prediction, the most important features classifying subjects into clusters were measures of obesity (waist to hip ratio, BMI, and visceral fat index), total body water, blood pressure, energy intake, total fat, olive oil intake, total fiber intake, and water intake. In conclusion, the SVM model was shown as a valuable tool to classify healthy individuals based on their gut microbiota composition.

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Section: Discussionsupporting

confidence: 67%

Obesity Measures and Dietary Parameters as Predictors of Gut Microbiota Phyla in Healthy Individuals

et al. 2020

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“…The species with the highest number of vitamin auxotrophies were F. prausnitzii , S. variabile , and the E. rectale / Roseburia group. Previous studies have found that increased consumption of B vitamins (in particular riboflavin, cobalamin, folate, and pyridoxine) is associated with a higher abundance of these gut bacteria ( 41 , 42 ). Intriguingly, these species are among the most prevalent and abundant species in the human gut microbiota ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Vitamin Biosynthesis by Human Gut Butyrate-Producing Bacteria and Cross-Feeding in Synthetic Microbial Communities

Soto-Martin

Warnke

Farquharson

et al. 2020

mBio

142

118

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We investigated the requirement of 15 human butyrate-producing gut bacterial strains for eight B vitamins and the proteinogenic amino acids by a combination of genome sequence analysis and in vitro growth experiments. The Ruminococcaceae species Faecalibacterium prausnitzii and Subdoligranulum variabile were auxotrophic for most of the vitamins and the amino acid tryptophan. Within the Lachnospiraceae, most species were prototrophic for all amino acids and several vitamins, but biotin auxotrophy was widespread. In addition, most of the strains belonging to Eubacterium rectale and Roseburia spp., but few of the other Lachnospiraceae strains, were auxotrophic for thiamine and folate. Synthetic coculture experiments of five thiamine or folate auxotrophic strains with different prototrophic bacteria in the absence and presence of different vitamin concentrations were carried out. This demonstrated that cross-feeding between bacteria does take place and revealed differences in cross-feeding efficiency between prototrophic strains. Vitamin-independent growth stimulation in coculture compared to monococulture was also observed, in particular for F. prausnitzii A2-165, suggesting that it benefits from the provision of other growth factors from community members. The presence of multiple vitamin auxotrophies in the most abundant butyrate-producing Firmicutes species found in the healthy human colon indicates that these bacteria depend upon vitamins supplied from the diet or via cross-feeding from other members of the microbial community. IMPORTANCE Microbes in the intestinal tract have a strong influence on human health. Their fermentation of dietary nondigestible carbohydrates leads to the formation of health-promoting short-chain fatty acids, including butyrate, which is the main fuel for the colonic wall and has anticarcinogenic and anti-inflammatory properties. A good understanding of the growth requirements of butyrate-producing bacteria is important for the development of efficient strategies to promote these microbes in the gut, especially in cases where their abundance is altered. The demonstration of the inability of several dominant butyrate producers to grow in the absence of certain vitamins confirms the results of previous in silico analyses. Furthermore, establishing that strains prototrophic for thiamine or folate (butyrate producers and non-butyrate producers) were able to stimulate growth and affect the composition of auxotrophic synthetic communities suggests that the provision of prototrophic bacteria that are efficient cross feeders may stimulate butyrate-producing bacteria under certain in vivo conditions.

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“…[38] It is also involved in one-carbon metabolism in the human gut. [39] Lactobacillus strains, S. thermophilus, and Ruminococcus bromii depend on B2 for their growth and metabolism. [40][41][42] Gut anaerobic and butyrate-producing bacteria F. prausnitzii and Butyricicoccus pullicaecorum use extracellular B2 to reduce oxygen via extracellular electron transport and can so cope with environmental oxygen.…”

Section: Vitamin B2-riboflavinmentioning

confidence: 99%

Role of Dietary Micronutrients on Gut Microbial Dysbiosis and Modulation in Inflammatory Bowel Disease

Kundra¹,

Rachmühl²,

Lacroix³

et al. 2021

Molecular Nutrition Food Res

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In patients with inflammatory bowel diseases (IBD), dietary micronutrient intake is low and deficiencies are common. Besides the host, also the gut microbiota require micronutrients and low levels may disturb its functioning. Multi‐omics studies indeed detected shifts in micronutrient‐dependent microbial pathways in IBD. It is however not clear whether micronutrients may alleviate inflammation directly, by modulating the immune system, or also indirectly, by modulating the structure and function of the gut microbiota. The latter seems of particular interest, since the gut microbiota is one of the future therapeutic targets in IBD. A review of the most recent available literature on relevant micronutrients in context of IBD and gut microbiota was conducted. An overview per relevant micronutrient on its role on gut bacterial growth, metabolism and host–microbe interactions during gut inflammation is provided. Dietary micronutrients have potential to be part of future personalized microbiome‐targeted therapies in IBD, considering both the micronutrient status of the host and the gut microbiota. However, cohort studies together with integrated multi‐scale studies are needed to understand the mechanisms of micronutrient–microbiome–host interactions in IBD and to evaluate efficacy and safety of dietary micronutrient treatment strategies.

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Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon

Cited by 57 publications

References 47 publications

Obesity Measures and Dietary Parameters as Predictors of Gut Microbiota Phyla in Healthy Individuals

Obesity Measures and Dietary Parameters as Predictors of Gut Microbiota Phyla in Healthy Individuals

Vitamin Biosynthesis by Human Gut Butyrate-Producing Bacteria and Cross-Feeding in Synthetic Microbial Communities

Role of Dietary Micronutrients on Gut Microbial Dysbiosis and Modulation in Inflammatory Bowel Disease

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