Dietary Oleocanthal Supplementation Prevents Inflammation and Oxidative Stress in Collagen-Induced Arthritis in Mice

Abstract

Oleocanthal (OLE), a characteristic and exclusive secoiridoid of Oleoaceae family, is mainly found in extra virgin olive oil (EVOO). Previous studies have reported its antioxidant, anti-inflammatory, antimicrobial, anticancer and neuroprotective effects. Since the pathogenesis of rheumatoid arthritis (RA) involves inflammatory and oxidative components, this study was designed to evaluate the preventive role of dietary OLE-supplemented effects in collagen-induced arthritis (CIA) murine model. Animals were fed with a preventive OLE-enriched dietary during 6 weeks previous to CIA induction and until the end of experiment time. At day 43 after first immunization, mice were sacrificed: blood was recollected and paws were histological and biochemically processed. Dietary OLE prevented bone, joint and cartilage rheumatic affections induced by collagen. Levels of circulatory matrix metalloproteinase (MMP)-3 and pro-inflammatory cytokines (IL-6, IL-1β, TNF-α, IL-17, IFN-γ) were significantly decreased in secoiridoid fed animals. Besides, dietary OLE was able to diminish COX-2, mPGES-1 and iNOS protein expressions and, also, PGE2 levels. The mechanisms underlying these protective effects could be related to Nrf-2/HO-1 axis activation and the inhibition of relevant signaling pathways including JAK-STAT, MAPKs and NF-κB, thus controlling the production of inflammatory and oxidative mediators. Overall, our results exhibit preliminary evidences about OLE, as a novel dietary tool for the prevention of autoimmune and inflammatory disorders, such as RA.

(−)-Methyl-Oleocanthal, a New Oleocanthal Metabolite Reduces LPS-Induced Inflammatory and Oxidative Response: Molecular Signaling Pathways and Histones Epigenetic Modulation

“…In the present study, we reported that met-OLE suppressed MAPKs’ phosphorylation in a concentration-dependent manner. These results are in line with those from our previous studies, which reported that MAPKs’ activation was drastically inhibited by OLE in LPS -induced murine macrophages [ 14 ], as well as in the paws of arthritic animals fed with this EVOO phenol [ 15 ].…”

“…OLE represents up to 10% of the total polyphenol content in EVOO (0.2–498 mg/kg) [ 12 ] and has received more scientific attention, due to its interesting biological activities, both within in vitro and in vivo systems, including anti-inflammatory, antioxidant, cardioprotective, chemopreventive and neuroprotective properties [ 10 , 13 ]. In fact, recently, we have reported the preventive role of dietary OLE-supplemented effects in a collagen-induced arthritis (CIA) murine model and the ability of OLE to diminish the acute inflammatory response in LPS-induced murine peritoneal macrophages [ 14 , 15 ].…”

Oleacein Attenuates Lipopolysaccharide-Induced Inflammation in THP-1-Derived Macrophages by the Inhibition of TLR4/MyD88/NF-κB Pathway

“…Several research groups reported the anti-oxidant and anti-inflammatory activities of oleocanthal and oleuropein [30][31][32][33][34][35][36][37], while those of OLC are still poorly defined. Recently, it has been shown that OLC exerts anti-inflammatory effects in human adipocytes challenged with TNF-α, a prototypic inflammatory stimulus, which could aid to explain the cardiometabolic benefit of EVOO consumption [32].…”

A Major Diplotaxis harra-Derived Bioflavonoid Glycoside as a Protective Agent against Chemically Induced Neurotoxicity and Parkinson’s Models; In Silico Target Prediction; and Biphasic HPTLC-Based Quantification

“…Cruciferae) are distributed worldwide, and many of them have been traditionally used as anti-arthritic [ 9 ] and antirheumatic [ 10 ]. In this context, plants of genus Diplotaxis provided supporting evidence for this traditional use as they exhibited anti-inflammatory [ 11 , 12 , 13 ] and antioxidant/antiradical [ 13 , 14 , 15 ] activities since inflammation and oxidative stress are linked to inflammatory movement disorders, including rheumatoid arthritis [ 16 , 17 , 18 ]. Genus Diplotaxis includes edible and medicinal plants [ 12 , 19 , 20 ] of about 30 species, mainly distributed in Central Europe, the Mediterranean region [ 21 ], and Southwest Asia [ 22 ], including Saudi Arabia [ 23 ].…”