Dietary Oxidized Linoleic Acid Modulates Plasma Lipids beyond Triglycerides Metabolism

Garelnabi, Mahdi; Ainsworth, Gregory; Mahini, Halleh; Jamil, Naseeha; Ochin, Chinedu

doi:10.1155/2017/1645828

Cited by 4 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No significant difference was seen between both treated groups, showing the comparable effect of both treatments in increasing HDL uptake. Previous studies have reported that treatment with LA enhanced HDL levels in human [29,30] and animal [44] studies. In this study, increased HDL uptake in the LA-treated HepG2 cells was attributed to increased mRNA expression of the SR-BI gene.…”

Section: Discussionmentioning

confidence: 90%

Xestospongia muta Fraction-7 and Linoleic Acid: Effects on SR-BI Gene Expression and HDL Cholesterol Uptake

Azemi

Abu-Bakar

et al. 2022

Marine Drugs

View full text Add to dashboard Cite

Xestospongia muta is a marine sponge belonging to the family Petrosiidae. It is an important source of biologically active marine natural products, with different kinds of essential fatty acids. Scavenger receptor class B type I (SR-BI) is the main receptor for high-density lipoprotein (HDL) cholesterol, which plays a pivotal role in preventing atherosclerosis. It removes cholesterol from HDL cholesterol, returning lipid-poor lipoprotein into blood circulation. The present study investigated the effects of X. muta Fraction-7 and linoleic acid on SR-BI gene expression and HDL cholesterol uptake. In vitro studies of the activity of X. muta and linoleic acid against the therapeutic target for hypercholesterolemia were conducted using the HDL receptor SR-BI via luciferase assay and HepG2 cells. In the present study, Fraction-7 of X. muta showed the highest expression level of the SR-BI gene via luciferase assay. Profiling of Fraction-7 of X. muta by GC-MS revealed 58 compounds, comprising various fatty acids, particularly linoleic acid. The in vitro study in HepG2 cells showed that the Fraction-7 of X. muta and linoleic acid (an active compound in X. muta) increased SR-BI mRNA expression by 129% and 85%, respectively, compared to the negative control. Linoleic acid increased HDL uptake by 3.21-fold compared to the negative control. Thus, the Fraction-7 of X. muta and linoleic acid have the potential to be explored as adjuncts in the treatment of hypercholesterolemia to prevent or reduce the severity of atherosclerosis development.

show abstract

Section: Discussionmentioning

confidence: 90%

Xestospongia muta Fraction-7 and Linoleic Acid: Effects on SR-BI Gene Expression and HDL Cholesterol Uptake

Azemi

Abu-Bakar

et al. 2022

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…This could be due to the LCFAs being major constituents of larger lipid molecules such as triglycerides and cholesterol esters within the liver and the blood. 27 28 The fatty acids in the livers of group A mice had significant higher concentrations than the concentration in the livers of the P group controls while the concentrations in the B group were statistically significantly less than the controls. This may be due to a dose dependent mechanism of oxidized LA associated with the biosynthesis and catabolism of fatty acids in the liver.…”

Section: Discussionmentioning

confidence: 92%

Dietary Oxidized Linoleic Acids Modulate Fatty Acids in Mice

Ochin

Wilson

Garelnabi

2022

J Lipid Atheroscler

Self Cite

View full text Add to dashboard Cite

Objective An elevated concentration of oxidized lipids along with the abnormal accumulation of lipids has been linked to the formation of atheromatous plaque and the development of cardiovascular diseases. This study aims to investigate if consumption of different concentrations of dietary oxidized linoleic acid alters the distribution of long chain fatty acids (LCFAs) within the liver relative to plasma in mice. Methods C57BL/6 male mice (n = 40) were divided into 4 groups: Standard chow as plain control (P group, n =10), Chow supplemented with linoleic acid 9 mg/mouse/day, linoleic control (C group, n=0), oxidized linoleic acid; 9 mg/mouse/day (A group, n=10) and oxidized linoleic acid 18 mg/mouse/day diet (B group, n=10). Liver and plasma samples were extracted, trans-esterified and subsequently analyzed using gas chromatography mass spectrometry (GC-MS) for LCFAs; palmitic acid, stearic acid, oleic acid, linoleic acid and arachidonic acid. Results LCFA methyl esters were eluted and identified based on their respective physiochemical characteristics of GCMS assay with inter assay coefficient of variation percentage (CV%, 1.81–5.28%), limits of quantification and limit of detection values (2.021–11.402 mg/mL and 1.016–4.430 mg/mL) respectively. Correlation analysis of liver and plasma lipids of the mice groups yielded coefficients (r=0.96, 0.6, 0.8 and 0.33) with fatty acid percentage total of (16%, 10%, 16% and 58%) for the P, C, A and B groups respectively. Conclusion The sustained consumption of a diet rich in oxidized linoleic acid disrupted fatty acid metabolism. The intake also resulted in elevated concentration of LCFAs that are precursors of bioactive metabolite molecule.

show abstract

“…The metabolic effect(s) of species such as oxidised triglycerides have not yet been characterised in infants. There is reasonable evidence to warrant the assumption that these compounds can have possible down-stream biological activity [50,51], for example, though agonism of PPAR receptors by oxidised fatty acids [52].…”

Section: Discussionmentioning

confidence: 99%

The Lipid and Glyceride Profiles of Infant Formula Differ by Manufacturer, Region and Date Sold

Furse

Koulman

2019

Nutrients

View full text Add to dashboard Cite

We tested the hypothesis that the lipid composition of infant formula is consistent between manufacturers, countries and target demographic. We developed techniques to profile the lipid and glyceride fraction of milk and formula in a high throughput fashion. Formula from principal brands in the UK (2017–2019; bovine-, caprine-, soya-based), the Netherlands (2018; bovine-based) and South Africa (2018; bovine-based) were profiled along with fresh British animal and soya milk and skimmed milk powder. We found that the lipid and glyceride composition of infant formula differed by region, manufacturer and date of manufacture. The formulations within some brands, aimed at different target age ranges, differed considerably where others were similar across the range. Soya lecithin and milk lipids had characteristic phospholipid profiles. Particular sources of fat, such as coconut oil, were also easy to distinguish. Docosahexaenoic acid is typically found in triglycerides rather than phospholipids in formula. The variety by region, manufacturer, date of manufacture and sub-type for target demographics lead to an array of lipid profiles in formula. This makes it impossible to predict its molecular profile. Without detailed profile of the formula fed to infants, it is difficult to characterise the relationship between infant nutrition and their growth and development.

show abstract

Dietary Oxidized Linoleic Acid Modulates Plasma Lipids beyond Triglycerides Metabolism

Cited by 4 publications

References 13 publications

Xestospongia muta Fraction-7 and Linoleic Acid: Effects on SR-BI Gene Expression and HDL Cholesterol Uptake

Xestospongia muta Fraction-7 and Linoleic Acid: Effects on SR-BI Gene Expression and HDL Cholesterol Uptake

Dietary Oxidized Linoleic Acids Modulate Fatty Acids in Mice

The Lipid and Glyceride Profiles of Infant Formula Differ by Manufacturer, Region and Date Sold

Contact Info

Product

Resources

About