Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

Abstract

Postbiotics from Lactobacillus plantarum have been reported to improve growth performance, nutrient utilization, immune status and gut health in livestock. However, there is scarce information on the antioxidant activity of postbiotics and its modulation of antioxidant activity and rumen barrier function in animals. We investigated the antioxidant activity of postbiotics from L. plantarum RG14, RG11 and TL1 and dietary effects in post-weaning lambs on serum and ruminal antioxidant activity, hepatic antioxidant enzymes and ruminal barrier function. Postbiotic RG14 showed the highest antioxidant activity in both 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay and was chosen to be evaluated in animal trials. Twelve post-weaning Dorper lambs were allotted to the control group and postbiotic group (0.9% (v/w) postbiotic RG14). The improvement in antioxidant activity of the postbiotic group was observed by greater glutathione peroxidase (GPX) in serum and ruminal fluid and lower serum TBARS. The findings were strengthened by the upregulation of hepatic GPX1, GPX4 and copper, zinc superoxide dismutase (Cu/Zn SOD) in the postbiotic group. Lambs received postbiotics had higher regulation of rumen barrier function through upregulation of tight junction protein (TJP), occludin (OCLD), claudin-1 (CLDN1) and CLDN4. The current study demonstrated that dietary postbiotics enhanced the serum and ruminal fluid antioxidant activity, reduced the serum lipid peroxidation and upregulated hepatic antioxidant enzymes and ruminal barrier function.

Influence of probiotics “Immunobacterin-D” on biocenoses and development of the gastrointestinal tract of calves

“…Пробіотики здатні змінювати рН рубця і виробляти антибіотичні речовини для того, щоб запобігти розмноженню патогенної мікрофлори та прикріпленню її до епітелію. Так, деякі види Lactobacillus мають сильну пригнічуючу активність на L. monocytogenes, S. thypimurium та E. coli (Izuddin et al, 2019;Izuddin et al, 2020). Дослідниками були виділені органічні кислоти -як метаболіти видів.…”

“…Отже, включення до випоювань молока ФПД "Імунобактерин-D" 1 раз/добу, щоденно, забезпечує надійну (100 %) профілактику розладів травлення (Izuddin et al, 2020).…”

“…На 30ту добу експерименту в дослідній групі чисельність бактерій вірогідно збільшилась на 94,3 %; інфузорійна 40,5 %; ентодиноморфів -на 26,7 % порівняно з контролем (Р < 0,001). За результатами проведеного експерименту можна стверджувати, що ферментнопробіотична добавка "Імунобактерин-D" збільшує кількісний і поліпшує якісний склад мікроорганізмів в рубці телят (Izuddin et al, 2020).…”

Supplementation of Postbiotic RI11 Improves Antioxidant Enzymes Activity, Upregulated Gut Barrier Genes and Reduced Cytokines, Acute Phase Proteins and HSP70 Gene Expression Levels in Heat-Stressed Broilers

“…Aside from developing a healthy gut and promote growth performance, a preliminary study from this laboratory revealed that the postbiotics produced by L. plantarum have high antioxidant activities [40]. Similarly, bacterial cultures of L. plantarum were reported to exhibit high antioxidative activities [45,46].…”

“…It has recently been observed that the dietary supplementation of postbiotics promoted the health and growth performance in broilers [31][32][33], layers [34,35] and piglets [36,37]. More recently, postbiotics have been reported enhancing the growth performance, rumen fermentation, immune status, antioxidant defence system, and gut health in small ruminants [38][39][40]. Under normal environmental temperature, dietary supplementation of postbiotics improved health and growth performance of broilers by promoting their immune status, growth genes expression and gut health as their supplementation significantly improved the intestinal villus, decreased the population of Enterobacteriaceae and faecal pH, and increased the population of lactic acid bacteria [25,[31][32][33]41].…”