Dietary selenium supplementation alleviates immune toxicity in the hearts of chickens with lead-added drinking water

Huang, He; Jiao, Xiaoyan; Xu, Yong; Han, Qin; Jiao, Wanying; Liu, Yanyan; Li, Shu; Teng, Xiaohua

doi:10.1080/03079457.2019.1572102

Cited by 33 publications

(17 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, Pb 2+ enhanced nitric oxide production in both nonactivated and activated HD‐11 macrophages in a concentration‐dependent manner (Figure 4). This could be related to the up‐regulation of inducible nitric oxide synthase by Pb 2+ exposure (Liu et al 2012; Chiang et al 2014; Huang et al 2019). However, when GSH synthesis was blocked by BSO, affecting the cells’ ability to protect itself from the nitric oxide–related oxidative burst, the cells produced less nitric oxide than the ones without BSO; hence it seems that the cell needs the protective antioxidants before it can mount an oxidative burst.…”

Section: Discussionmentioning

confidence: 99%

Modulatory Effects of Pb²⁺ on Virally Challenged Chicken Macrophage (HD‐11) and B‐Lymphocyte (DT40) Cell Lines In Vitro

Han

García-Mendoza

Berg

et al. 2020

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Elevated levels of lead have been found in waterfowl, due to human activities. Lead may cause immunomodulatory effects, but the mechanisms are largely unknown, especially after viral challenges. To characterize avian immunomodulatory hazards of lead (Pb)2+, we used chicken macrophage (HD‐11) and B‐lymphocyte (DT40) cell lines, as in vitro models for the innate and adaptive immune systems, respectively. The cells were activated via toll‐like receptor‐3 by polyinosinic–polycytidylic acid sodium salt (poly I:C), mimicking viral infections. Our results indicate that Pb2+ is cytotoxic to both cell lines, macrophages being more sensitive. De novo synthesis of glutathione plays an important role in protecting macrophages from Pb2+ intoxication, which might also be closely involved in the induction of nitric oxide after Pb2+ exposure. Stimulatory effects on cell proliferation were noticed at noncytotoxic Pb2+ concentrations as well. Exposure to Pb2+ could also affect the inflammatory status by inhibiting the pro‐inflammatory interferon (IFN)‐γ while promoting the production of anti‐inflammatory type I IFNs in both macrophages and B‐cells, and increasing intracellular IgM levels in B‐cells. These results suggest that the immunomodulatory effects of Pb2+ in birds are probably closely associated with disruption of immune cell proliferation and cytokine production, potentially causing disorders of the avian immune system. Environ Toxicol Chem 2020;39:1060–1070. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC

show abstract

Section: Discussionmentioning

confidence: 99%

Modulatory Effects of Pb²⁺ on Virally Challenged Chicken Macrophage (HD‐11) and B‐Lymphocyte (DT40) Cell Lines In Vitro

Han

García-Mendoza

Berg

et al. 2020

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…Effects of selenium on adaptive and innate immunity Selenium supplementation has been noted to stimulate T-cell proliferation and enhance innate immune-system functions [ 71 ]. Supplementation of 200 μg/day (as sodium selenite) for 8 weeks caused large increases in cytotoxic T cells and Natural Killer (NK) cells by upregulating receptors for the growth regulatory lymphokine, interleukin-2 and consequently, the rate of cell proliferation and differentiation into cytotoxic cells [ 72 ].…”

Section: Introductionmentioning

confidence: 99%

Selenium and selenoproteins in viral infection with potential relevance to COVID-19

et al. 2020

View full text Add to dashboard Cite

Selenium is a trace element essential to human health largely because of its incorporation into selenoproteins that have a wide range of protective functions. Selenium has an ongoing history of reducing the incidence and severity of various viral infections; for example, a German study found selenium status to be significantly higher in serum samples from surviving than non-surviving COVID-19 patients. Furthermore, a significant, positive, linear association was found between the cure rate of Chinese patients with COVID-19 and regional selenium status. Moreover, the cure rate continued to rise beyond the selenium intake required to optimise selenoproteins, suggesting that selenoproteins are probably not the whole story. Nonetheless, the significantly reduced expression of a number of selenoproteins, including those involved in controlling ER stress, along with increased expression of IL-6 in SARS-CoV-2 infected cells in culture suggests a potential link between reduced selenoprotein expression and COVID-19-associated inflammation. In this comprehensive review, we describe the history of selenium in viral infections and then go on to assess the potential benefits of adequate and even supra-nutritional selenium status. We discuss the indispensable function of the selenoproteins in coordinating a successful immune response and follow by reviewing cytokine excess, a key mediator of morbidity and mortality in COVID-19, and its relationship to selenium status. We comment on the fact that the synthetic redox-active selenium compound, ebselen, has been found experimentally to be a strong inhibitor of the main SARS-CoV-2 protease that enables viral maturation within the host. That finding suggests that redox-active selenium species formed at high selenium intake might hypothetically inhibit SARS-CoV-2 proteases. We consider the tactics that SARS-CoV-2 could employ to evade an adequate host response by interfering with the human selenoprotein system. Recognition of the myriad mechanisms by which selenium might potentially benefit COVID-19 patients provides a rationale for randomised, controlled trials of selenium supplementation in SARS-CoV-2 infection.

show abstract

“…Heat shock response can protect against toxicity caused by excess heavy metals [48]. Previous studies reported that Pb increased HSPs (HSP27, HSP40, HSP60, HSP70, and HSP90 mRNA expression in peripheral blood neutrophils [49] and hearts [50] of chickens. In the present study, we observed high expression of HSPs (HSP27, HSP40, HSP60, HSP70, and HSP90) mRAN and protein caused by Pb exposure in the chicken kidneys, re ected the activation of this intracellular buffer system, which responds to oxidative stress when the antioxidant enzyme (T-AOC, GST, and CAT) exhaustion occurs [46].…”

Section: Discussionmentioning

confidence: 98%

“…Preparation of sections described in our present papers was processed as previously described [22]. Brie y, the kidney tissues xed with paraformaldehyde solution were dehydrated in gradient alcohol (30,50,70,90, 100, and 100%), were embedded in para n, and were sectioned to nominal thicknesses of 4 µm. The sections were stained with hematoxylin and eosin.…”

Section: Histological Analysesmentioning

confidence: 99%

Se Alleviated Oxidative Stress-Mediated Complex Poisoning Mechanism in Pb-Treated Chicken Kidneys: Inflammation, Heat Shock Response, and Autophagy

Miao

Wang

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Lead (Pb) is a toxic environmental pollutant and can exerts toxicity in kidneys. It is known that selenium (Se) has an antagonistic effect on Pb poisoning. However, biological events during the process were not well understood in chicken kidneys.Methods: One hundred and eighty male Hyline chickens (7-day-old) were randomly divided into the control group (offering standard diet and potable water), the Se group (offering Na2SeO3-added standard diet and potable water), the Pb group (offering standard diet and (CH3OO)2Pb-added potable water), and the Pb+Se group (offering Na2SeO3-added standard diet and (CH3OO)2Pb-added potable water). On 30th, 60th, and 90th days, kidneys were removed to perform the studies of histological structure, oxidative stress indicators, cytokines, heat shock proteins, and autophagy in the chicken kidneys.Results: The experimental results indicated that Pb poisoning changed renal histological structure; decreased catalase, glutathione-s-transferase, and total antioxidative capacity activities; increased hydrogen peroxide content; induced mRNA and protein expression of heat shock proteins; inhibited interleukin (IL)-2 mRNA expression, and induced IL-4 and IL-12β mRNA expression; inhibited mammalian target of rapamycin mRNA and protein expression, and induced autophagy-related gene mRNA and protein expression in the chicken kidneys. Supplement of Se mitigated the above changes caused by Pb.Conclusion: Our research strengthens the evidence that Pb induced oxidative stress, inflammation, heat shock response, and autophagy and Se administration alleviated Pb poisoning through mitigating oxidative stress in the chicken kidneys.

show abstract

Dietary selenium supplementation alleviates immune toxicity in the hearts of chickens with lead-added drinking water

Cited by 33 publications

References 43 publications

Modulatory Effects of Pb²⁺ on Virally Challenged Chicken Macrophage (HD‐11) and B‐Lymphocyte (DT40) Cell Lines In Vitro

Modulatory Effects of Pb²⁺ on Virally Challenged Chicken Macrophage (HD‐11) and B‐Lymphocyte (DT40) Cell Lines In Vitro

Selenium and selenoproteins in viral infection with potential relevance to COVID-19

Se Alleviated Oxidative Stress-Mediated Complex Poisoning Mechanism in Pb-Treated Chicken Kidneys: Inflammation, Heat Shock Response, and Autophagy

Contact Info

Product

Resources

About

Dietary selenium supplementation alleviates immune toxicity in the hearts of chickens with lead-added drinking water

Cited by 33 publications

References 43 publications

Modulatory Effects of Pb2+ on Virally Challenged Chicken Macrophage (HD‐11) and B‐Lymphocyte (DT40) Cell Lines In Vitro

Modulatory Effects of Pb2+ on Virally Challenged Chicken Macrophage (HD‐11) and B‐Lymphocyte (DT40) Cell Lines In Vitro

Selenium and selenoproteins in viral infection with potential relevance to COVID-19

Se Alleviated Oxidative Stress-Mediated Complex Poisoning Mechanism in Pb-Treated Chicken Kidneys: Inflammation, Heat Shock Response, and Autophagy

Contact Info

Product

Resources

About

Modulatory Effects of Pb²⁺ on Virally Challenged Chicken Macrophage (HD‐11) and B‐Lymphocyte (DT40) Cell Lines In Vitro

Modulatory Effects of Pb²⁺ on Virally Challenged Chicken Macrophage (HD‐11) and B‐Lymphocyte (DT40) Cell Lines In Vitro