Dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via RENT/AKT/IRS/GLUT4 pathway

Abstract

Background Gestational diabetes mellitus (GDM) has significant short and long-term health consequences for both the mother and child. There is limited but suggestive evidence that inulin could improve glucose tolerance during pregnancy. This study assessed the effect of inulin on glucose homeostasis and elucidated the molecular mechanisms underlying the inulin-induced antidiabetic effects during pregnancy. Method Female C57BL/6 mice were randomized to receive either no treatment, high-dose inulin and low-dose inulin for 7 weeks with measurement of biochemical profiles. A real-time2 (RT2) profiler polymerase chain reaction (PCR) array involved in glycolipid metabolism was measured. Results Inulin treatment facilitated glucose homeostasis in a dose-dependent manner by decreasing fasting blood glucose, advanced glycation end products and total cholesterol, and improving glucose tolerance. Suppressing resistin (RETN) expression was observed in the inulin treatment group and the expression was significantly correlated with fasting blood glucose levels. The ratios of p-IRS to IRS and p-Akt to Akt in liver tissue and the ratio of p-Akt to Akt in adipose tissue as well as the expression level of GLUT4 increased significantly after inulin treatment. Conclusions Our findings indicated improvement of glucose and lipid metabolism by inulin was to activate glucose transport through the translocation of GLUT4 which was mediated by insulin signaling pathway repairment due to decreased expression of RETN and enhanced phosphorylation of IRS and Akt in GDM mice.

Dietary inulin supplementation promote hepatic gluconeogenesis in goats

“…Inulin is a dietary ber that regulates glucose metabolism [23]. The observed reduction in blood insulin content and the consistent levels of glucagon and glucose in this study suggest that inulin attenuates insulin secretion from pancreatic β-cells Miao et al reported that 3.33 and 1.67 g/kg/day inulin supplementation signi cantly reduced blood glucose and insulin levels in gestational diabetes mellitus mice [24]. Similar results were reported by Zhang et al [25]that blood glucose and insulin levels in high-fat mice were signi cantly reduced when supplemented with 2 g/kg of inulin.…”

The Protective Effects of Inulin-Type Fructans Against High-Fat/Sucrose Diet-Induced Gestational Diabetes Mice in Association With Gut Microbiota Regulation

“…In human intervention studies, doses ranging from 12 to 16 g/day are often given when testing for metabolic effects of ITF ( Cani et al, 2009 ; Parnell and Reimer, 2009 ; Tarini and Wolever, 2010 ; Rahat-Rozenbloom et al, 2017 ), which equals to 2.46 and 3.33 g/kg body weight in mice, respectively ( Nair and Jacob, 2016 ). In this study, the dose was based on our previously published study of ITF administration in mice ( Miao et al, 2021 ). A high dose was chosen due to the short window of treatment allowed by pregnancy.…”

The Synergetic Effect of Egyptian Portulaca oleracea L. (Purslane) and Cichorium intybus L. (Chicory) Extracts against Glucocorticoid-Induced Testicular Toxicity in Rats through Attenuation of Oxidative Reactions and Autophagy

“…Inulin is a polymer of fructose with a β-(2-1)-glycosidic bond, is low in calories, and can be used as a sugar substitute [ 37 ]. It is also an excellent component of a diabetic diet and was reported to improve glucose and lipid metabolism [ 136 , 137 , 138 ]. Moreover, Rahimiyan-Heravan, Roshangar [ 138 ] reported the potential therapeutic effects of inulin on serum and testicular reproductive markers in diabetic male rats.…”