This study was planned and conducted to comprehensively evaluate the attitudes and behaviors of university students regarding probiotic/prebiotic sources and supplements. A three-part questionnaire form was developed to assess the attitudes and behaviors of participants regarding probiotics/prebiotics. The probiotic sources mostly consumed everyday were cheese types, yogurt, and buttermilk. In contrast, 95.2% of the participants had never consumed boza, 92.8% vinegar, and 61.6% kefir. As for the consumed prebiotic sources were analyzed, 15.2% of the participants consumed whole-grain/whole-wheat bread, 7.9% dried fruits, and 5.8% bananas everyday. The prebiotic sources that were almost never consumed were asparagus, soybeans, and artichokes. The rates of probiotic and prebiotic consumption for a specific reason were 18.3% and 16.2%, respectively. When asked about the specific reasons for consuming probiotic/prebiotic sources, the most common response was strengthening immunity. The rate of participants who used probiotic supplements was 4.5%, whereas none of the participants reported using prebiotic supplements. The reason for using probiotic supplements was mostly strengthening immunity. Brief information on probiotics and prebiotics provided in written and visual media may not be sufficient. It is important that information and awareness-raising activities are planned by the relevant authorities for the conscious choice of these sources and supplements.