“…Rotifers and Artemia nauplii have been widely used as live feeds for larval fish during the past decade, but they are deficient in n‐3 polyunsaturated fatty acids (PUFA) for some species (Watanabe, Oowa, Kitajima & Fujita 1978). Several methods to increase the n‐3 PUFA content in live feeds, such as enrichment of live feeds by ω‐yeast (Kitajima, Yoshida & Watanabe 1980), emulsifying oil (Watanabe, Tamiya, Oka, Hirata, Kitajima & Fujita 1983a; Gatesoupe 1991; Ozkizilcik & Chu 1994), microalgae (Watanabe, Oowa, Kitajima, Fujita & Yone 1979; Hayashi, Toda, Yoneji, Sato & Kitaoka 1993; Ozkizilcik & Chu 1994), self‐emulsifying product, SELCO (Dhert, Lavens, Duray & Sorgeloos 1990; Kraul, Nelson, Brittain, Ako & Ogasawara 1992; Stottrup & Attramadal 1992), microencapsules (Walford & Lam 1987; Ozkizilcik & Chu 1994; Southgate & Lou 1995) and fish by‐products, silages containing ω‐3 PUFA (Tocher & Harvie 1988; Tocher, Mourente & Sargent 1997) have been developed and are effective in improving growth and survival rates of many fish species. Specifically, docosahexaenoic acid (DHA, 22:6n‐3) and eicosapentaenoic (EPA, 20:5n‐3) acids are known to be essential for visual and neural functioning and brain development of fish (Mourente, Tocher & Sargent 1991; Bell, Batty, Navarro, Sargent & Dick 1995).…”