Dietary vitamin D3 deprivation suppresses fibroblast growth factor 23 signals by reducing serum phosphorus levels in laying hens

Yan, Jiakun; Pan, Chong; Liu, Yan Li; Liao, Xujie; Chen, Jionghao; Zhu, Yong; Huang, Xinhuo; Yang, Xiaojun; Ren, Zhouzheng

doi:10.1016/j.aninu.2021.07.010

Cited by 3 publications

(3 citation statements)

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“…In humans, mutations in this gene have been associated with hypercalcemia, hypercalciuria, hypophosphatemia [71], and idiopathic infantile hypercalcemia [72]. In laying hens, some studies evaluated the CYP24A1 gene to verify its role in osteogenesis [73] and in the regulation of serum vitamin D [31,74]. Gloux et al [31] observed that kidney CYP24A1 upregulation occurred earlier in old than in young hens at 18-19 h post ovulation, attributing this result to the need to stimulate Ca retention and bone resorption.…”

Section: Discussionmentioning

confidence: 99%

CYP24A1 and TRPC3 Gene Expression in Kidneys and Their Involvement in Calcium and Phosphate Metabolism in Laying Hens

Salmória,

Ibelli,

Tavernari

et al. 2024

Animals

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Ca and P homeostasis across the egg-laying cycle is a complex process involving absorption in the small intestine, reabsorption/excretion in the kidneys, and eggshell gland secretion. Diets with inadequate calcium and phosphorus can interfere with their absorption and digestibility, resulting in eggshell quality losses and reduced productive life, affecting egg production and welfare. A better understanding of gene expression profiles in the kidneys of laying hens during the late egg-laying period could clarify the renal role in mineral metabolism at this late stage. Therefore, the performance, egg quality and bone integrity-related traits, and expression profiles of kidney candidate genes were evaluated in 73-week-old laying hens receiving different Ca and P ratios in their diet: a high Ca/P ratio (HR, 22.43), a low ratio (LR, 6.71), and a medium ratio (MR, 11.43). The laying hens receiving the HR diet had improved egg production and eggshell quality traits compared to the other two groups. Humerus length was shorter in the HR than in the other groups. The CYP24A1 and TRPC3 genes were differentially expressed (p.adj ≤ 0.05) among the groups. Therefore, their expression profiles could be involved in calcium and phosphate transcellular transport in 73-week-old laying hens as a way to keep mineral absorption at adequate levels.

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Section: Discussionmentioning

confidence: 99%

CYP24A1 and TRPC3 Gene Expression in Kidneys and Their Involvement in Calcium and Phosphate Metabolism in Laying Hens

Salmória,

Ibelli,

Tavernari

et al. 2024

Animals

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“…A second, more tightly regulated hydroxylation occurs in the kidney at the 1α-carbon to form 1,25(OH) 2 D 3 ( Jones et al, 1998 ). In mammals and fish, this is carried out by an enzyme encoded by CYP27B1 ( Monkawa et al, 1997 ; Shinki et al, 1997 ; Chun et al, 2014 ); however, this gene has not been identified in chickens and the enzyme responsible is currently unknown despite recent publications that have reported measuring expression of CYP27B1 mRNA or an equivalent ( Shanmugasundaram and Selvaraj, 2012 ; Gloux et al, 2020a ; Gloux et al, 2020b ; Yan et al, 2022 ). Investigation into transcripts amplified reveals these are an enzyme involved in retinoic acid metabolism ( CYP27C1 ) or one identified as vitamin D 3 hydroxylase-associated protein ( Ettinger et al, 1994 ; Ettinger and DeLuca, 1995 ), neither of which have demonstrable 1α-hydroxylase activity.…”

Section: Vitamin D 3 Metabolism and Mechanism Of A...mentioning

confidence: 99%

Physiological regulation of calcium and phosphorus utilization in laying hens

Sinclair-Black

Garcia²,

Ellestad³

2023

Front. Physiol.

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Commercial laying hens can produce one egg approximately every 24 h. During this process, regulatory systems that control vitamin D3 metabolism, calcium and phosphorus homeostasis, and intestinal uptake of these minerals work in concert to deliver components required for eggshell calcification and bone mineralization. Commercial production cycles have been extended in recent years to last through 100 weeks of age, and older hens often exhibit an increased prevalence of skeletal fractures and poor eggshell quality. Issues such as these arise, in part, through imbalances that occur in calcium and phosphorus utilization as hens age. As a result, an in-depth understanding of the mechanisms that drive calcium and phosphorus uptake and utilization is required to develop solutions to these welfare and economic challenges. This paper reviews factors that influence calcium and phosphorus homeostasis in laying hens, including eggshell formation and development and roles of cortical and medullary bone. Metabolism and actions of vitamin D3 and physiological regulation of calcium and phosphorus homeostasis in key tissues are also discussed. Areas that require further research in avian species, such as the role of fibroblast growth factor 23 in these processes and the metabolism and action of bioactive vitamin D3, are highlighted and the importance of using emerging technologies and establishing in vitro systems to perform functional and mechanistic studies is emphasized.

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“…The protein isolation and western blotting procedures were conducted as previously described [29]. Primary antibodies (rabbit) to type 2a sodium-phosphate cotransporter (NPt2a, catalogue no.…”

Section: Western-blotting Analysismentioning

confidence: 99%

Adjusting phosphate feeding regimen according to daily rhythm increases eggshell quality via enhancing medullary bone remodeling in laying hens

Yan

Wang

Chen

et al. 2023

J Animal Sci Biotechnol

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Background Body phosphorus metabolism exhibits a circadian rhythm over the 24-h daily cycle. The egg laying behavior makes laying hens a very special model for investigating phosphorus circadian rhythms. There is lack of information about the impact of adjusting phosphate feeding regimen according to daily rhythm on the phosphorus homeostasis and bone remodeling of laying hens. Methods and results Two experiments were conducted. In Exp. 1, Hy-Line Brown laying hens (n = 45) were sampled according the oviposition cycle (at 0, 6, 12, and 18 h post-oviposition, and at the next oviposition, respectively; n = 9 at each time point). Diurnal rhythms of body calcium/phosphorus ingestions and excretions, serum calcium/phosphorus levels, oviduct uterus calcium transporter expressions, and medullary bone (MB) remodeling were illustrated. In Exp. 2, two diets with different phosphorus levels (0.32% and 0.14% non-phytate phosphorus (NPP), respectively) were alternately presented to the laying hens. Briefly, four phosphorus feeding regimens in total (each included 6 replicates of 5 hens): (1) fed 0.32% NPP at both 09:00 and 17:00; (2) fed 0.32% NPP at 09:00 and 0.14% NPP at 17:00; (3) fed 0.14% NPP at 09:00 and 0.32% NPP at 17:00; (4) fed 0.14% NPP at both 09:00 and 17:00. As a result, the regimen fed 0.14% NPP at 09:00 and 0.32% NPP at 17:00, which was designed to strengthen intrinsic phosphate circadian rhythms according to the findings in Exp. 1, enhanced (P < 0.05) MB remodeling (indicated by histological images, serum markers and bone mineralization gene expressions), elevated (P < 0.05) oviduct uterus calcium transportation (indicated by transient receptor potential vanilloid 6 protein expression), and subsequently increased (P < 0.05) eggshell thickness, eggshell strength, egg specific gravity and eggshell index in laying hens. Conclusions These results underscore the importance of manipulating the sequence of daily phosphorus ingestion, instead of simply controlling dietary phosphate concentrations, in modifying the bone remodeling process. Body phosphorus rhythms will need to be maintained during the daily eggshell calcification cycle. Graphical Abstract

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Dietary vitamin D3 deprivation suppresses fibroblast growth factor 23 signals by reducing serum phosphorus levels in laying hens

Cited by 3 publications

References 55 publications

CYP24A1 and TRPC3 Gene Expression in Kidneys and Their Involvement in Calcium and Phosphate Metabolism in Laying Hens

CYP24A1 and TRPC3 Gene Expression in Kidneys and Their Involvement in Calcium and Phosphate Metabolism in Laying Hens

Physiological regulation of calcium and phosphorus utilization in laying hens

Adjusting phosphate feeding regimen according to daily rhythm increases eggshell quality via enhancing medullary bone remodeling in laying hens

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