Difference between cholic acid and chenodeoxycholic acid in dependence upon cholesterol of hepatic and plasmatic sources as the precursor in rats

Ayaki, Yoshikazu; Ogura, Yoshio; Kitayama, S; Endo, Saburo; Ogura, Michio

doi:10.1016/0039-128x(83)90091-0

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…This result in conventional rats corresponds well to the increase of chenodeoxycholic acid in the feces in cholestyramine- Y. IMAI ET treated germ-free rats (4). Chenodeoxycholic acid has been reported to be transformed into ~-muricholic acid through a-muricholic acid in bile fistula rats (28,29) and isolated hepatocytes (30,31). The first step of this conversion is 6~-hydroxylation of chenodeoxycholic acid.…”

Section: Fecal Sterol and Bile Acid Determination Fecal Sterolsmentioning

confidence: 99%

Effect of cholestyramine on bile acid metabolism in conventional rats

Imai

Kawata

Inada

et al. 1987

Lipids

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Effects of cholestyramine on biliary secretion of cholesterol, phospholipids and bile acids and fecal excretion of sterols and bile acids were examined in Wistar male rats. Six rats were fed a basal diet, and the other six were fed a basal diet supplemented with 5% cholestyramine for eight days. Bile flow and biliary secretion of bile acids and phospholipids (per hour per rat) decreased with cholestyramine treatment, while biliary cholesterol secretion (per hour per rat) remained unchanged. In the biliary bile acid composition, a marked increase of chenodeoxycholic acid with a concomitant decrease of beta-muricholic acid was observed in cholestyramine-treated rats. Fecal excretion of total sterols and bile acids increased about three- and four-fold, respectively, after cholestyramine treatment. The increase of fecal bile acids derived from cholic acid was more predominant than that derived from chenodeoxycholic acid, resulting in an increase of the cholic acid group/chenodeoxycholic acid group ratio.

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Section: Fecal Sterol and Bile Acid Determination Fecal Sterolsmentioning

confidence: 99%

Effect of cholestyramine on bile acid metabolism in conventional rats

Imai

Kawata

Inada

et al. 1987

Lipids

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“…This finding is supported by our previous study (37) and also by recently published work (14), demonstrating that a bolus of mevinolin inhibited chenodeoxycholate and muricholate to a greater extent than it did cholate. Some investigators using the tritiated water method (36), the [180,1 inhalation technique (21) or various cholesterol precursors (26,30,31,36) came to the same conclusion. In contrast to these findings, others (23-25) suggested a common cholesterol precursor pool for cholate and chenodeoxycholate in human beings and rats.…”

Section: Discussionmentioning

confidence: 79%

“…In contrast to biliary cholesterol, only few data are available for the precursor pools of individual bile acids. Earlier studies with various labeled precursors other than tritiated water or oxygen suggested that cholesterol and bile acids are derived from the same (23)(24)(25) or from separate (26)(27)(28)(29)(30)(31)(32) cholesterol pools in different species. However, using these older techniques, only differences in specific activities of secreted bile acids and biliary cholesterol were detectable, but the amount or proportion of bile acids synthesized from newly formed cholesterol could not be quantitated.…”

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confidence: 99%

Bile acid synthesis from newly synthesized vs. preformed cholesterol precursor pools in the rat

et al. 1993

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The present study defines the origin of cholesterol subserving bile acid synthesis in male rats with an extracorporal bile duct by labeling newly formed cholesterol with tritiated water. Within 6 hr after interruption of the enterohepatic circulation, the bile acid pool was depleted. At this early time point the proportion from de novo cholesterol was 8% and 12% for biliary cholesterol and cholate, but 18% and 19% for muricholate and chenodeoxycholate, respectively. This proportion gradually rose to 40%, 34%, 51% and 51%, respectively, at 15 to 30 hr. At 78 hr after bile diversion, 64% of cholate was labeled, compared with 84% to 88% of the other biliary lipids and 71% of plasma cholesterol. Total and labeled bile acid secretion exhibited the same diurnal rhythm. To allow differentiation between direct hepatocytic de novo synthesis of bile acids from acetate and recycling of labeled plasma cholesterol, an inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase (pravastatin) was infused from 54 to 78 hr. It suppressed total synthesis of primary bile acids by 60% to 80% but decreased the tritium label of bile acids only from a range of 74% to 92% (54 hr) to a range of 54% to 63% (78 hr), which was in the range of plasma cholesterol (58%). We conclude that bile acids and biliary cholesterol are synthesized mostly from preformed (i.e., plasma) cholesterol, both immediately after depletion of the pool in enterohepatic circulation and after derepression. Moreover, the hepatic cholesterol pools subserving the synthesis of different bile acids and biliary cholesterol secretion are not identical.

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“…However, they may stem from relatively long-lasting metabolic changes in the liver, since they were ob served in animals fasted for 15 h. The en hanced output of bile acids, in animals on the apple diet might be a result of an en hanced cholesterol 7a-hydroxylase activity [12], the key enzyme for bile acid biosynthe sis from cholesterol. There may also be a more available pool of substrate since he patic cholesterogenesis remains relatively ac tive in animals on the fruit diet despite the 15-hour period of fasting [11], and it has been shown that neoformed cholesterol is a preferential substrate for bile acid synthesis [27], especially that of CA [28].…”

Section: Discussionmentioning

confidence: 99%

Steroid Pattern of Bile and Feces in Response to a Fruit-Enriched Diet in Hypercholesterolemic Hamsters

Sablé

Sicart²,

Berry³

1990

Ann Nutr Metab

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The effect of a fruit (apple)-enriched diet on bile secretion and on fecal steroid excretion was studied in two strains of hamster: normal hamsters with normal cholester-olemia and spontaneous hypercholesterolemic hamsters with high-level hepatic cholesterol esters (FEC hamsters). Quantitative and qualitative alterations in the steroid composition in bile and feces were accompanied by changes in intestinal morphology. The fruit diet displayed a choleretic effect and increased the output of bile acids from liver in FEC hamsters. In addition, bile collected continuously from cannulated apple-fed animals was enriched with conjugates of cholic acid. Moreover, apple consumption lowered the lithogenic index of the bile. In response to the fruit diet, fecal excretion of bile acids and neutral sterols increased, essentially in the form of primary bile acids and sterol esters, respectively.

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Difference between cholic acid and chenodeoxycholic acid in dependence upon cholesterol of hepatic and plasmatic sources as the precursor in rats

Cited by 5 publications

References 16 publications

Effect of cholestyramine on bile acid metabolism in conventional rats

Effect of cholestyramine on bile acid metabolism in conventional rats

Bile acid synthesis from newly synthesized vs. preformed cholesterol precursor pools in the rat

Steroid Pattern of Bile and Feces in Response to a Fruit-Enriched Diet in Hypercholesterolemic Hamsters

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