Difference in activity and gene expression of pectin-degrading enzymes during softening process in two cultivars of Chinese pear fruit

Jian-mei, Wei; Qi, Xin; Cheng, Yudou; Guan, Junfeng

doi:10.1016/j.scienta.2015.10.002

Cited by 31 publications

(17 citation statements)

References 45 publications

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“…The decrease of flesh firmness is not only a symbol of fruit ripening but also evidence of senescence in many fruits. 38 texture, which depends on respiration, cellular water and degradation of pectin substances, is another important trait used to evaluate the quality of fruit. The decrease of flesh firmness is mainly caused by cell wall degradation and water loss.…”

Section: Discussionmentioning

confidence: 99%

Application of sodium silicate retards apple softening by suppressing the activity of enzymes related to cell wall degradation

Duan

et al. 2018

J Sci Food Agric

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BACKGROUND During the storage of apples, apple softening is one of the main problems. Sodium silicate has been used to enhance disease resistance and maintain quality of fruits. In the present study, apple fruit (cv. Golden delicious) were treated with 100 mmol L–1 sodium silicate for 10 min and stored at 20 °C to investigate its effects on weight loss, flesh firmness, and the activity of cell wall‐degrading enzymes. RESULTS The results indicated that 100 mmol L–1 of sodium silicate treatment delayed the increase of weight loss and decrease of the flesh firmness in apples. Sodium silicate treatment also suppressed the activity of polygalacturonic acid transeliminase and pectin methyltranseliminase, pectin methylgalacturonase, polygalacturonase, cellulase and β‐galactosidase in the fruit. CONCLUSIONS Delaying apple softening by sodium silicate treatment is closely related to the inhibition of the activity of cell wall‐degrading enzymes and weight loss. © 2018 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Application of sodium silicate retards apple softening by suppressing the activity of enzymes related to cell wall degradation

Duan

et al. 2018

J Sci Food Agric

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“…UDP-glucuronate-4-epimerase, UDP-glucose-6-dehydrogenase, pectinesterase (PE) and polygalacturonase (PG) have been recognized as key enzymes in generating and deconstructing pectin [ 74 ]. Using the fruit firmness tester, we found that firmness declined significantly after 25 DAA.…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-based Protein Profiling and Fruit Quality Changes at Different Development Stages of Oriental Melon

Guo

Cui

et al. 2017

BMC Plant Biol

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BackgroundOriental melon is one of the most popular crops for its nutritional and flavour quality. Components that determine melon quality, such as sugar, colour, texture, flavour and aroma, among other factors, accumulate in different developmental stages. Thus, correlating the proteomic profiles with the biochemical and physiological changes occurring in the oriental melon is very important for advancing our understanding of oriental melon quality in the ripening processes.ResultsiTRAQ-based protein profiling was conducted on ‘YuMeiren’ oriental melon fruit at different developmental stages. Physiological quality indices, including firmness, rind colour, soluble solids content (SSC), ethylene production, sugar content and volatile compounds were also characterized during four maturity periods of the melon, including 5, 15, 25 and 35 days after anthesis (DAA). A principal component analysis (PCA) revealed that the aroma volatiles at 5 DAA and 15 DAA were similar and separated from that of 35 DAA. More than 5835 proteins were identified and quantified in the two biological repeats and divided into 4 clusters by hierarchical cluster analysis. A functional analysis was performed using Blast2GO software based on the enrichment of a GO analysis for biological process, molecular function and cellular components. The main KEGG pathways, such as glycolysis, α-linolenic acid and starch and sucrose metabolism, were analyzed.The gene family members corresponding to differentially expressed proteins, including lipoxygenase (CmLOX01-18) and alcohol acetyltransferase (CmAAT1-4) involved in the α-linolenic acid metabolic pathway, were verified with real-time qPCR. The results showed that the expression patterns of 64.7% of the genes were consistent with the expression patterns of the corresponding proteins.ConclusionsThis study combined the variation of the quality index and differentially expressed proteins of oriental melon at different developmental stages that laid the foundation for the subsequent protein and gene function validation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-0977-7) contains supplementary material, which is available to authorized users.

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“…UDP-glucose 6-dehydrogenase was identified as a member of the sucrose metabolism pathway (Table 3 ). UDP-glucose 6-dehydrogenase is a pectin-degrading enzyme (Wei et al, 2015 ). Fruit firmness is closely related to soluble pectin content, and up-regulation of UDP-glucose 6-dehydrogenase expression may thus lead to fruit softening (Fischer and Bennett, 1991 ).…”

Section: Discussionmentioning

confidence: 99%

A Transcriptome Approach Toward Understanding Fruit Softening in Persimmon

et al. 2017

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Persimmon (Diospyros kaki Thunb.), which is a climacteric fruit, softens in 3–5 weeks after harvest. However, little is known regarding the transcriptional changes that underlie persimmon ripening. In this study, high-throughput de novo RNA sequencing was performed to examine differential expression between freshly harvested (FH) and softened (ST) persimmon fruit peels. Using the Illumina HiSeq platform, we obtained 259,483,704 high quality reads and 94,856 transcripts. After the removal of redundant sequences, a total of 31,258 unigenes were predicted, 1,790 of which were differentially expressed between FH and ST persimmon (1,284 up-regulated and 506 down-regulated in ST compared with FH). The differentially expressed genes (DEGs) were further subjected to KEGG pathway analysis. Several pathways were found to be up-regulated in ST persimmon, including “amino sugar and nucleotide sugar metabolism.” Pathways down-regulated in ST persimmon included “photosynthesis” and “carbon fixation in photosynthetic organisms.” Expression patterns of genes in these pathways were further confirmed using quantitative real-time RT-PCR. Ethylene gas production during persimmon softening was monitored with gas chromatography and found to be correlated with the fruit softening. Transcription involved in ethylene biosynthesis, perception and signaling was up-regulated. On the whole, this study investigated the key genes involved in metabolic pathways of persimmon fruit softening, especially implicated in increased sugar metabolism, decreased photosynthetic capability, and increased ethylene production and other ethylene-related functions. This transcriptome analysis provides baseline information on the identity and modulation of genes involved in softening of persimmon fruits and can underpin the future development of technologies to delay softening in persimmon.

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Difference in activity and gene expression of pectin-degrading enzymes during softening process in two cultivars of Chinese pear fruit

Cited by 31 publications

References 45 publications

Application of sodium silicate retards apple softening by suppressing the activity of enzymes related to cell wall degradation

Application of sodium silicate retards apple softening by suppressing the activity of enzymes related to cell wall degradation

iTRAQ-based Protein Profiling and Fruit Quality Changes at Different Development Stages of Oriental Melon

A Transcriptome Approach Toward Understanding Fruit Softening in Persimmon

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