Differences between Male Hormone Extracts from Urine and from Testes

Comparison of the Hershberger assay and androgen receptor binding assay of twelve chemicals

“…Assays to detect androgenic properties have been developed since the 1930s (Korenchevsky, 1932;Korenchevsky et al, 1932Korenchevsky et al, , 1933aBülbring and Burn, 1935;Dingemanse et al, 1935;Deanesly and Parkes, 1936;Wainman and Shipounoff, 1941;Eisenberg et al, 1949;Eisenberg and Gordan, 1950;Di Salle et al, 1994), and the capabilities of the assays were demonstrated in 1953 by Hershberger et al when they analyzed the response of the ventral prostate, seminal vesicles and coagulating glands, and the levator ani without the bulbocavernosus muscle to a number of active chemicals, including estrogens and progesterones (Hershberger et al, 1953).…”

Comparative activities of compounds of the androsterone-testosterone series

“…In the meantime evidence had been accumulating that the male hormone activity of male urine differed quantitatively from that of testicular extracts, notably in its smaller activity per capon unit on the seminal vesicles of castrated rats (see Dingemanse et al [1935] for refs. ), and it was soon evident that androsterone was not the chief active principle of testicular extracts [David and Freud, 1935;Callow and Deanesly, 1935, 1].…”

Effect of androsterone and of male hormone concentrates on the accessory reproductive organs of castrated rats, mice and guinea-pigs

“…Moore and Gallagher [1930,2], enumerating mammalian indicators for male hormone, state that, after the spermatozoon motility test (one not in general use), the restoration of normal prostatic structure is the most sensitive test, the next being cytological restoration of the seminal vesicles. Many tests of this kind have been made, but although histological changes in the glandular epithelium have been brought about by a few days' treatment, yet the stimulation or mnaintenance of glands of normal adult size in groups of animals castrated for as long as 10 or 14 days has not been quantitatively demonstrated, until recently by Dingemanse et al [1935]. These workers give the average seminal vesicle weights in 5 immature castrated rats injected for 24 days with a testis extract as 538 mg., approximately equivalent to the glands in a normal 175 g. rat ( Fig.…”