Differences between strains of rhizobium in sensitivity to canavanine

Weaks, T. E.

doi:10.1007/bf02187248

Cited by 7 publications

(8 citation statements)

References 19 publications

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“…We observed that L-canavanine delays the growth of S. meliloti in a concentration-dependent manner. This is in agreement with previously recorded observations (51). In order to study the effect on expE2 gene expression independent of the delayed growth rate, we grew all cultures to early logarithmic phase, before addition of AHLs and L-canavanine.…”

Section: Discussionsupporting

confidence: 90%

“…However, the synthesis of L-canavanine commences with the emergence of the very first leaf, indicating that it may not function solely as a storage metabolite. It has been suggested that L-canavanine may act as an allelopathic substance, since it is known to be a potent inhibitor of growth of several microorganisms and phytophagous insects (40,51).…”

Section: Discussionmentioning

confidence: 99%

“…L-Canavanine is an arginine analog that inhibits the growth of certain bacteria, including some strains of S. meliloti (11,51). However, an effect on gene regulation has not been recorded to date.…”

mentioning

confidence: 99%

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l-Canavanine Made byMedicago sativaInterferes with Quorum Sensing inSinorhizobium meliloti

et al. 2005

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Sinorhizobium meliloti is a gram-negative soil bacterium, capable of establishing a nitrogen-fixing symbiosis with its legume host, alfalfa (Medicago sativa). Quorum sensing plays a crucial role in this symbiosis, where it influences the nodulation process and the synthesis of the symbiotically important exopolysaccharide II (EPS II). S. meliloti has three quorum-sensing systems (Sin, Tra, and Mel) that use N-acyl homoserine lactones as their quorum-sensing signal molecule. Increasing evidence indicates that certain eukaryotic hosts involved in symbiotic or pathogenic relationships with gram-negative bacteria produce quorum-sensing-interfering (QSI) compounds that can cross-communicate with the bacterial quorum-sensing system. Our studies of alfalfa seed exudates suggested the presence of multiple signal molecules capable of interfering with quorum-sensingregulated gene expression in different bacterial strains. In this work, we choose one of these QSI molecules (SWI) for further characterization. SWI inhibited violacein production, a phenotype that is regulated by quorum sensing in Chromobacterium violaceum. In addition, this signal molecule also inhibits the expression of the S. meliloti exp genes, responsible for the production of EPS II, a quorum-sensing-regulated phenotype. We identified this molecule as L-canavanine, an arginine analog, produced in large quantities by alfalfa and other legumes.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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l-Canavanine Made byMedicago sativaInterferes with Quorum Sensing inSinorhizobium meliloti

et al. 2005

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“…39 L-canavanine is known to serve as an allelopathic substance by inhibiting the growth of certain bacteria. 40 Handelsman et al showed that canavanine has the potential to affect the population biology of Bacillus cereus.…”

Section: L-canavanine As a Quorum-sensing Inhibitormentioning

confidence: 99%

Quorum Sensing in Plaque Biofilms: Challenges and Future Prospects

Biradar¹,

Devi²

2011

The Journal of Contemporary Dental Practice

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“…It has been reported to be as abundant as up to 5% (dry weight) of some leguminous seeds (149). In addition to serving as a nitrogen source for the germinating seedlings, L-canavanine is also known to serve as an allelopathic substance by inhibiting the growth of certain bacteria and phytophagous insects (119).…”

Section: L-canavanine As a Quorum-sensing Inhibitormentioning

confidence: 99%

Messing with Bacterial Quorum Sensing

Gonzalez

Keshavan

2006

Microbiol Mol Biol Rev

377

232

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SUMMARY Quorum sensing is widely recognized as an efficient mechanism to regulate expression of specific genes responsible for communal behavior in bacteria. Several bacterial phenotypes essential for the successful establishment of symbiotic, pathogenic, or commensal relationships with eukaryotic hosts, including motility, exopolysaccharide production, biofilm formation, and toxin production, are often regulated by quorum sensing. Interestingly, eukaryotes produce quorum-sensing-interfering (QSI) compounds that have a positive or negative influence on the bacterial signaling network. This eukaryotic interference could result in further fine-tuning of bacterial quorum sensing. Furthermore, recent work involving the synthesis of structural homologs to the various quorum-sensing signal molecules has resulted in the development of additional QSI compounds that could be used to control pathogenic bacteria. The creation of transgenic plants that express bacterial quorum-sensing genes is yet another strategy to interfere with bacterial behavior. Further investigation on the manipulation of quorum-sensing systems could provide us with powerful tools against harmful bacteria.

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Differences between strains of rhizobium in sensitivity to canavanine

Cited by 7 publications

References 19 publications

l-Canavanine Made byMedicago sativaInterferes with Quorum Sensing inSinorhizobium meliloti

l-Canavanine Made byMedicago sativaInterferes with Quorum Sensing inSinorhizobium meliloti

Quorum Sensing in Plaque Biofilms: Challenges and Future Prospects

Messing with Bacterial Quorum Sensing

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