Differences between the effects of plant species and compartments on microbiome composition in two halophyte Suaeda species

Abstract

Root-related or endophytic microbes in halophytes play an important role in adaptation to extreme saline environments. However, there have been few comparisons of microbial distribution patterns in different tissues associated with halophytes. Here, we analyzed the bacterial communities and distribution patterns of the rhizospheres and tissue endosphere in two Suaeda species ( S. salsa and S. corniculata Bunge) using the 16S rRNA gene sequencing. The results showed that the bacterial abundance and diversity in the rhizosphere were significantly higher than that of endophytic, but lower than that of bulk soil. Microbial-diversity analysis showed that the dominant phyla of all samples were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria and Firmicutes , among which Proteobacteria were extremely abundant in all the tissue endosphere. Heatmap and Linear discriminant analysis Effect Size (LEfSe) results showed that there were notable differences in microbial community composition related to plant compartments. Different networks based on plant compartments exhibited distinct topological features. Additionally, the bulk soil and rhizosphere networks were more complex and showed higher centrality and connectedness than the three endosphere networks. These results strongly suggested that plant compartments, and not species, affect microbiome composition.

The Marine Bacterial Genus Euzebya Is Distributed Worldwide in Terrestrial Environments: A Review

“…The occurrence of Euzebya in these environments has been reported in numerous studies [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55], denoting the ability of the members of this genus to prosper in habitats with high salt concentrations (Table 2).…”

“…In other highly saline environments, such as salt marshes, mines, and lake soils, Euzebya was detected in the rhizosphere of halophytic plants [41,43,[47][48][49]54].…”

Insight into bacterial and archaeal community structure ofSuaeda altissimaandSuaeda dendroidesrhizosphere in response to different salinity level

“…They play a crucial role in nutrient cycling, organic matter decomposition, and enhancing plant resistance to abiotic and biotic stress to maintain productivity ( 37 ). However, the diversity and community structure of halophyte-associated rhizosphere microbes depend on the soil properties and plant species ( 38 ). Our research highlights the importance of understanding the complex interplay between soil properties, plant species, and microbial communities in saline soils.…”

“…It can be seen that not only related to soil characteristics, but also plant physiological activity can influence the microbial community structure. Plant species have been proved that it can affect the structural and functional diversity of rhizosphere microbial community due to variations in root exudation and rhizodeposition in different zones ( 38 , 46 ). In our study, we also confirmed this viewpoint and found significant differences in the structure of bacterial and archaeal community structures in the rhizosphere between S. altissima and S. dendroides ( Fig.…”

Plant Compartments Shape the Assembly and Network of Vallisneria natans-Associated Microorganisms

“…The endophytic bacterial community's niche differentiation is low due to the host plant's space and nutrient limitations. Similarly, sediment microbial communities exhibit niche differentiation, which may result from root deposition effects, leading to the selection of specific bacteria combinations [42]. The networks of V. natans' various samples were positively correlated, indicating that most bacterial colonies had extensive cooperative and symbiotic potential in their respective compartments.…”