Differences between the Large and Small Intestine in the Immunodominant Amoebic Proteins Recognized by IgG and IgA Antibodies in BALB/c Mice

Abstract

We have previously reported that there are differences in the number of predominant amoebic antigens recognized by serum and small intestinal antibodies induced after local and systemic immunization with glutarldehyde-fixed Entamoeba histolytica trophozoites (GFT) in BALB/c mice, by an immunoblot analysis. Moreover, by enzyme-linked immunosorbent assay (ELISA) analysis, we found differences in the antiamoebic antibody isotype patterns elicited at the large and small intestines. To further characterize the antiamoebic immune response induced in BALB/c mice, after local (oral and rectal) and systemic (intraperitoneal and intramuscular) immunization with GFT, we performed an immunoblot analysis of the amoebic proteins predominantly recognized by immunoglobulins (Ig)G, IgA and IgM in the serum and in the small and large intestines. The present work shows differences between the large and small intestine in the IgG-and IgA-antibody recognition pattern of amoebic proteins, thus confirming and extending our previous findings supporting the compartmentalization of the intestinal immune response. Furthermore, our reported observation that there are differences in the amoebic proteins predominantly recognized by antibodies of different isotypes was extended to the intestines, as some proteins with relative molecular weights of 24±25, 66, 140 kDa are strongly recognized by IgG but not by other antibody isotypes.

Striking phenotypic and functional differences in lamina propria lymphocytes from the large and small intestine of mice

“…Responses induced at different mucosal sites can vary depending on the antigen and route of immunization (Moreno-Fierros et al, 2000, 2003, Hopkins et al, 1995. In the case of intestinal immunity there are clear differences between the LI and the SI on the quality and intensity of the response as well as on the recognition pattern of antibodies induced by local and systemic immunization (Moreno-Fierros et al, 2002).…”

Strategic Compartmentalization of Toll-Like Receptor 4 in the Mouse Gut

“…A few studies have addressed the compartmentalization of the immune response in the mouse gut, but none of them measured PRRs (19,20). We have now shown that TLR4, CD14, and TLR2 are constitutively expressed in the mouse gut mucosa and that this expression varies along the gut.…”