Striking phenotypic and functional differences in lamina propria lymphocytes from the large and small intestine of mice

Amelioration of 2,4,6-trinitrobenzene sulfonic acid-induced colitis in mice by immunoregulatory dendritic cells

“…Small intestine (SI)-derived DC-enriched cells were isolated as previously described [1,8]. The cells were stained with fluorescein isothiocyanate (FITC)-conjugated mAb against CD11c (BD Biosciences, San Jose, CA), phycoerythrin (PE)-conjugated anti-CD11c, -CD103, PIR-A/B (BD Biosciences), PIR-B (clone 326414: R&D Systems, Inc., Minneapolis, USA), and allophycocyanin (APC)-conjugated anti-CD45R (B220) (BD Biosciences), -CD11b, -CD83 mAbs (eBioscience Inc., San Diego, CA), -CD40, -CD86, and -CD197 mAbs (CCR7), Alexa Fluor 647-conjugated anti-CD54 mAbs, PE/Cy7-conjugated anti-CD86 mAbs (BioLegend, San Diego, CA, USA), -CD45R (B220) (BD Biosciences), PIR-A/B (BD Biosciences), and anti-CX3CR1 Abs (ProSci inc., Flint Place Poway, CA, USA).…”

Regionalization of pIgR expression in the mucosa of mouse small intestine

“…The isolation of intestinal lymphocytes was carried out as previously described [9]. Briefly each intestinal segment was removed, carefully cleaned from its mesentery and flushed of fecal contents with cold RPMI medium.…”

“…TCR␥␦ + , CD2 − T and CD8 + T cells predominate in the SI, whereas TCR␣␤ + , CD2 + T and CD4 + T cells predominate in the LI [4][5][6]. Furthermore, there is evidence of differences in number and phenotype of the LPL in the LI and SI [7][8][9].…”

The effect of oral tolerance on the roles of small intestinal intraepithelial lymphocytes in murine colitis induced by dextran sodium sulfate

“…Small intestinal intraepithelial lymphocytes were prepared according to a modification of previously published methods [30]. The small intestine included the duodenal bulb to the ileocecal junction.…”