DIFFERENCES BETWEEN THE MUSCLES IN K FREE RINGER AND THOSE IN Ca FREE RINGER WITH SPECIAL REFERENCE TO IONIC BEHAVIORS

Effects of Mechanical and Chemical Disorganization of the Outermost Structure of the Muscle Fibres on the Release of Ions From the Muscle

“…Ionic changes produced by treatment with trypsin are due to disorganization of membrane structure which is supposed to cause the loosening of normal membrane substances which in turn results in the change in ionic contents . Previously, the present author (Noda, 1966) reported that trypsin and transmembrane movement of K or Na ions in Ca free Ringer are not related and the site of trypsin action may be far from the location of Ca++ in the membrane. It is obvious from the results, that the effect of trypsin is not the result of decalcifying of the membrane structure .…”

“…The present author (Noda, 1966) suggested both the heterogeneous distribution of internal rations of the muscle fibres and the possible role of the structural components in maintaining internal ion concentrations, and emphasized that the amount of internal K or Na ion concentration, which was obtained flame-photometrically, could not give the pictorial image of the intracellular state as a whole. In this piper, the effect of the outermost cellular structure of the muscle fibres on ions leaking out from or accumulating in the muscle has been investigated by applying passive stretch and by treating with trypsin or hypertonic Ringer.…”

“…The author (Noda, 1966) reported that K loss and Na gain of the muscle were brought about by the change of the process to extrude sodium ions. Thus, the movement of Na ions in trypsin-Ringer was investigated using radioactive Na ions.…”

“…In regard to the change in ion content, the author (Noda, 1966) suggests two types of ionic behaviors as in K free Ringer and in Ca free Ringer, the distinction being essential. The effect of trypsin on cationic movement, as described in this paper, resembled the ionic change in K free Ringer while the effect of hypertonic Na solution resulted in almost the same as in Ca free Ringer.…”

“…Radioactive sodium, potassium or calcium was used for efflux experiments of these ions. The methods utilized for this purpose were similar to those described in the previous paper (Noda, 1966). In particular, the change in the outermost structure of the cell membrane was interpreted in connection with the movement of membrane calcium.…”