The pot experiment was established in vegetation hall in the year 2015. Spring barley, variety KWS Irina, was grown. Two different soils -chernozem from Brno (with a low phosphorus content and alkali soil reaction -7.37) and haplic luvisol from Jaroměřice nad Rokytnou (with a high phosphorus content and slightly acid soil reaction -6.01) were used for comparison. The rates of phosphorus in the form of triple superphosphate (45 % P2O5) were increased from 0.3 -0.6 -1.2 g per pot (5 kg of soil -Mitscherlich pots). Nitrogen was applied in the form of CAN (27 % N) at a rate of 1 g N per pot in all the treatments incl. the control. Using statistical analysis, significant differences were found between the two soil types both in terms of the postharvest soil P content and yields of aboveground biomass. The content of post-harvest soil phosphorus increased significantly with the applied rate (96 -141 -210 mg/kg in chernozem and 128 -179 -277 mg/kg in haplic luvisol). Dry matter yields of the aboveground biomass grown on chernozem were the lowest in the control treatment not fertilised with P (38.97 g per pot) and increased significantly with the P rate applied (46.02 -47.28 g per pot), although there were no significant differences among the fertilised treatments. On haplic luvisol phosphorus fertilisation was not seen at all, demonstrating that the weight of the biomass in all the treatments was balanced (48.12 -49.63 g per pot).