Differences in Ca2+ regulation for high-output Is and low-output Ib motor terminals in Drosophila larvae

He, Tao; Singh, Vinay Kumar; Rumpal, Nidhi; Lnenicka, Gregory A.

doi:10.1016/j.neuroscience.2009.01.074

Cited by 23 publications

(47 citation statements)

References 35 publications

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“…7D). The higher [Ca 2+ ] c in type-Is boutons is consistent with a higher per-volume-density of active zones in phasic MN terminals, as reported previously in Drosophila (Meinertzhagen et al, 1998; He et al, 2009) and crayfish (Msghina et al, 1999). …”

Section: Resultssupporting

confidence: 91%

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Presynaptic Mitochondria in Functionally Different Motor Neurons Exhibit Similar Affinities for Ca²⁺But Exert Little Influence as Ca²⁺Buffers at Nerve Firing RatesIn Situ

Chouhan¹,

Zhang²,

Zinsmaier³

et al. 2010

J. Neurosci.

109

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Section: Resultssupporting

confidence: 91%

“…Mitochondria could have different thresholds (set points) for Ca 2+ sequestration. Alternatively, [Ca 2+ ] c could rise higher in type-Is than Ib terminals for a given stimulation frequency, although this was previously discounted (Karunanithi et al, 1997; Reiff et al, 2002; but see Rossano and Macleod, 2007, and He et al, 2009). …”

Section: Resultsmentioning

confidence: 99%

Presynaptic Mitochondria in Functionally Different Motor Neurons Exhibit Similar Affinities for Ca²⁺But Exert Little Influence as Ca²⁺Buffers at Nerve Firing RatesIn Situ

Chouhan¹,

Zhang²,

Zinsmaier³

et al. 2010

J. Neurosci.

109

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“…As in our previous studies (He et al, 2009; Lnenicka et al, 2006a), we loaded the indicator into the motor terminals using the technique developed by Macleod et al, 2002. Briefly, larvae were dissected in Schneider’s media (Sigma, St. Louis, MO) and the nerve supplying the third segment was cut.…”

Section: Methodsmentioning

confidence: 99%

“…As in a previous study, we estimated the [OGB-1] in the synaptic terminals by measuring the fluorescence intensity for a range of OGB-1 concentrations in capillary tubes with an inside diameter of approximately 20 μm (He et al, 2009). The fluorescent values were normalized to a 20 μm diameter giving a linear plot of [OGB-1] versus fluorescent intensity.…”

Section: Methodsmentioning

confidence: 99%

Chronic lead exposure alters presynaptic calcium regulation and synaptic facilitation in Drosophila larvae

Hirsch

Ruden

et al. 2009

NeuroToxicology

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Prolonged exposure to inorganic lead (Pb 2+ ) during development has been shown to influence activity-dependent synaptic plasticity in the mammalian brain, possibly by altering the regulation of intracellular Ca 2+ concentration ([Ca 2+ ] i ). To explore this possibility, we studied the effect of Pb 2+ exposure on [Ca 2+ ] i regulation and synaptic facilitation at the neuromuscular junction of larval Drosophila. Wild-type Drosophila (CS) were raised from egg stages through the third larval instar in media containing either 0, 100 μM or 250 μM Pb 2+ and identified motor terminals were examined in late third-instar larvae. To compare resting [Ca 2+ ] i and the changes in [Ca 2+ ] i produced by impulse activity, the motor terminals were loaded with a Ca 2+ indicator, either Oregon Green 488 BAPTA-1 (OGB-1) or fura-2 conjugated to a dextran. We found that rearing in Pb 2+ did not significantly change the resting [Ca 2+ ] i nor the Ca 2+ transient produced in synaptic boutons by single action potentials (APs); however, the Ca 2+ transients produced by 10 and 20 Hz AP trains were larger in Pb 2+ -exposed boutons and decayed more slowly. For larvae raised in 250 μM Pb 2+ , the increase in [Ca 2+ ] i during an AP train (20 Hz) was 29% greater than in control larvae and the [Ca 2+ ] i decay τ was 69% greater. These differences appear to result from reduced activity of the plasma membrane Ca 2+ ATPase (PMCA), which extrudes Ca 2+ from these synaptic terminals. These findings are consistent with studies in mammals showing a Pb 2+ -dependent reduction in PMCA activity. We also observed a Pb 2+ -dependent enhancement of synaptic facilitation at these larval neuromuscular synapses. Facilitation of EPSP amplitude during AP trains (20 Hz) was 55% greater in Pb 2+ -reared larvae than in controls. These results showed that Pb 2+ exposure produced changes in the regulation of [Ca 2+ ] i during impulse activity, which could affect various aspects of nervous system development. At the mature synapse, this altered [Ca 2+ ] i regulation produced changes in synaptic facilitation that are likely to influence the function of neural networks.

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“…Interestingly, in motor terminals of Drosophila larvae, PMCA2 makes little contribution to the function of the high release probability Is terminals compared with the lower output Ib terminals [22] .…”

Section: Bmentioning

confidence: 99%

Contribution of plasma membrane Ca²⁺ATPase to cerebellar synapse function

Huang

Nagaraja²,

Garside³

et al. 2010

WJBC

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The cerebellum expresses one of the highest levels of the plasma membrane Ca 2+ ATPase, isoform 2 in the mammalian brain. This highly efficient plasma membrane calcium transporter protein is enriched within the main output neurons of the cerebellar cortex; i.e. the Purkinje neurons (PNs). Here we review recent evidence, including electrophysiological and calcium imaging approaches using the plasma membrane calcium ATPase 2 (PMCA2) knockout mouse, to show that PMCA2 is critical for the physiological control of calcium at cerebellar synapses and cerebellar dependent behaviour. These studies have also revealed that deletion of PMCA2 throughout cerebellar development in the PMCA2 knockout mouse leads to permanent signalling and morphological alterations in the PN dendrites. Whilst these findings highlight the importance of PMCA2 during cerebellar synapse function and development, they also reveal some limitations in the use of the PMCA2 knockout mouse and the need for additional experimental approaches including cell-specific and reversible manipulation of PMCAs. THE CEREBELLUM, THE PURKINJE NEURON AND THE IMPORTANCE OF CALCIUM DYNAMICS AT CEREBELLAR SYNAPSESThe cerebellum is a major centre for the integration of sensory and motor information in the brain and plays a central role in our ability to learn and refine motor tasks; the specialised function of the cerebellum allows us to execute motor tasks in a finely controlled but, at the same time, "unaware" manner that can still be improved by learning. For a detailed review of cerebellar function TOPIC HIGHLIGHTWorld J Biol Chem 2010 May 26; 1(5): 95-102 ISSN 1949-8454 (online)

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Differences in Ca2+ regulation for high-output Is and low-output Ib motor terminals in Drosophila larvae

Cited by 23 publications

References 35 publications

Presynaptic Mitochondria in Functionally Different Motor Neurons Exhibit Similar Affinities for Ca²⁺But Exert Little Influence as Ca²⁺Buffers at Nerve Firing RatesIn Situ

Presynaptic Mitochondria in Functionally Different Motor Neurons Exhibit Similar Affinities for Ca²⁺But Exert Little Influence as Ca²⁺Buffers at Nerve Firing RatesIn Situ

Chronic lead exposure alters presynaptic calcium regulation and synaptic facilitation in Drosophila larvae

Contribution of plasma membrane Ca²⁺ATPase to cerebellar synapse function

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Differences in Ca2+ regulation for high-output Is and low-output Ib motor terminals in Drosophila larvae

Cited by 23 publications

References 35 publications

Presynaptic Mitochondria in Functionally Different Motor Neurons Exhibit Similar Affinities for Ca2+But Exert Little Influence as Ca2+Buffers at Nerve Firing RatesIn Situ

Presynaptic Mitochondria in Functionally Different Motor Neurons Exhibit Similar Affinities for Ca2+But Exert Little Influence as Ca2+Buffers at Nerve Firing RatesIn Situ

Chronic lead exposure alters presynaptic calcium regulation and synaptic facilitation in Drosophila larvae

Contribution of plasma membrane Ca2+ATPase to cerebellar synapse function

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Product

Resources

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Presynaptic Mitochondria in Functionally Different Motor Neurons Exhibit Similar Affinities for Ca²⁺But Exert Little Influence as Ca²⁺Buffers at Nerve Firing RatesIn Situ

Presynaptic Mitochondria in Functionally Different Motor Neurons Exhibit Similar Affinities for Ca²⁺But Exert Little Influence as Ca²⁺Buffers at Nerve Firing RatesIn Situ

Contribution of plasma membrane Ca²⁺ATPase to cerebellar synapse function