Differences in circulating dendritic cell subtypes in peripheral, placental and cord blood in African pregnant women

Aldebert, Delphine; Diallo, Mawlouth; Niang, Makhtar; Sarr, Demba; Cisse, C Boni; Moreau, J. C.; Jambou, Ronan

doi:10.1016/j.jri.2006.05.002

Cited by 20 publications

(33 citation statements)

References 28 publications

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“…As different DC subtypes are known to play an important role in eliciting Th1 and Th2 responses, considerable effort have been made to identify differences in the DC subtypes of cord blood and maternal blood. Despite this, however, the results have been conflicting (12-19). This can be partly explained by the use of different detection procedures and antibodies (22, 23).…”

Section: Discussionmentioning

confidence: 99%

“…The subtypes of dendritic cells from whole blood samples were analyzed as previously described (19, 20). A total of 50 µL of whole blood was incubated with optimum amounts (as indicated in the instructions of the manufacturer) of mouse anti-human CD123-PE, CD11c-APC, HLA-DR-PerCP and a lineage cocktail 1-fluorescein isothiocyanate (Lin1-FITC) (containing a mixture of FITC-conjugated anti-CD3, anti-CD14, anti-CD16, anti-CD19, anti-CD20 and anti-CD56 antibodies) (BD Biosciences, San Jose, CA, U.S.A.) for 25 min at room temperature in the dark.…”

Section: Methodsmentioning

confidence: 99%

“…Among the DC population, cells expressing CD11c were characterized as myeloid DC: CD11c + /CD123 ± (P3 gate), whereas cells expressing CD123 with high intensity were characterized as lymphoid DC: CD11c - /CD123 +++ (P5 gate). Cells expressing CD123 with low intensity were characterized as less differentiated DC: CD11c - /CD123 ± (P4 gate), as previously described (12, 19). Results were expressed as a percentage of total DC to ANC (all nucleated cells) and PBMC (peripheral blood mononuclear cells).…”

Section: Methodsmentioning

confidence: 99%

“…A recently reported third population of dendritic cells, referred to as CD11c - /CD123 ± , is thought to be a less differentiated DC population (10-12). Although a number of studies have tried to correlate the changes in immune status during pregnancy or in neonates with changes in DC subtype populations in the peripheral blood of pregnant women or in their cord blood, the results have been inconsistent (12-19) and there is no available data in Korean population.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, in order to minimize the in vitro effect of cell-preparation, we employed a direct immunofluorescence technique on whole blood samples and four-color flow cytometry (19, 20) to evaluate the proportion of each DC subtype in the peripheral blood of pregnant women at the time of delivery, in their umbilical cord blood, and in the peripheral blood of age-matched healthy, non-pregnant women in Korean population.…”

Section: Introductionmentioning

confidence: 99%

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Differences in Circulating Dendritic Cell Subtypes in Pregnant Women, Cord Blood and Healthy Adult Women

Shin

Jang²,

Roh

et al. 2009

J Korean Med Sci

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Different subtypes of dendritic cells (DC) influence the differentiation of naíve T lymphocytes into T helper type 1 (Th1) and Th2 effector cells. We evaluated the percentages of DC subtypes in peripheral blood from pregnant women (maternal blood) and their cord blood compared to the peripheral blood of healthy non pregnant women (control). Circulating DC were identified by flow cytometry as lineage (CD3, CD14, CD16, CD19, CD20, and CD56)-negative and HLA-DR-positive cells. Subtypes of DC were further characterized as myeloid DC (CD11c+/CD123±), lymphoid DC (CD11c-/CD123+++) and less differentiated DC (CD11c-/CD123±). The frequency of DC out of all nucleated cells was significantly lower in maternal blood than in control (P<0.001). The ratio of myeloid DC/lymphoid DC was significantly higher in maternal blood than in control (P<0.01). HLA-DR expressions of myeloid DC as mean fluorescence intensity (MFI) were significantly less in maternal blood and in cord blood than in control (P<0.001, respectively). The DC differentiation factors, TNF-α and GM-CSF, released from mononuclear cells after lipopolysaccharide stimulation were significantly lower in maternal blood than in control (P<0.01). The distribution of DC subtypes was different in maternal and cord blood from those of non-pregnant women. Their role during pregnancy remains to be determined.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Differences in Circulating Dendritic Cell Subtypes in Pregnant Women, Cord Blood and Healthy Adult Women

Shin

Jang²,

Roh

et al. 2009

J Korean Med Sci

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Nonhuman Primate Models of Immunosenescence

Rivera¹,

Rais²,

Barr³

et al. 2017

Handbook of Immunosenescence

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Due to a dramatic increase in life expectancy, the number of individuals aged 65 and older is rapidly rising. This presents considerable challenges to our health care system since advanced age is associated with a higher susceptibility to infectious diseases due to immune senescence. However, the mechanisms underlying age-associated dysregulated immunity are still incompletely understood. Advancement in our comprehension of mechanisms of immune senescence and development of interventions to improve health span requires animal models that closely recapitulate the physiological changes that occur with aging in humans. Nonhuman primates (NHPs) are invaluable preclinical models to study the underlying causal mechanism of pathogenesis due to their outbred nature, high degree of genetic and physiological similarity to humans, and their susceptibility to human pathogens. In this chapter, we review NHP models available for biogerontology research, advantages and challenges they present, and advances they facilitated. Furthermore, we emphasize the utility of NHPs in characterizing immune senescence, evaluating interventions to reverse aging of the immune system, and development of vaccine strategies that are better suited for this vulnerable population.

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