DIFFERENCES IN CYTOCOMPATIBILITY BETWEEN MWCNTs AND CARBOXYLIC FUNCTIONALIZED MWCNTs

Zhang, Yiteng; Li, Dejun; Zhao, Mengli; Guo, Meixian; Deng, Xiangyun; Gu, Haitao; Wan, Rongxin

doi:10.1142/s1793604712500531

Cited by 7 publications

(10 citation statements)

References 17 publications

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“…These studies were conducted on neuronal cells [ 11 , 13 ], osteoblasts [ 12 ], and fibroblasts [ 15 ] from human and rat sources. When the MWCNTs were functionalized, the presence of COOH groups enhances cell proliferation, viability, and adhesion due to the -COOH groups rendering the surface hydrophilic and wettable [ 14 ]. Carboxyl functionalized CNTs (CNT–COOH) have been used as an effective template to chemically synthesize HAp [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…As discussed earlier, COOH group promotes matrix mineralization. The presence of -COOH groups likely increased differentiation on day 7 [ 14 ] and matrix mineralization after day 28 due to increased hydrophilicity, surface wettability of MWCNTs and facilitating mineral matrix deposition [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…A cell adhesion study on MWCNTs and MWCNTs chemically functionalized with carboxylic acid groups (-COOH) on Ti discs using mouse fibroblast cells (L929) and human umbilical vein endothelial cells (EAHY926) showed that the MWCNT-COOH group had enhanced cell proliferation, viability, and adhesion due to enhanced wettability; indicating superior cyto-compatibility over MWCNTs [ 14 ]. When mouse fibroblasts (L929) were cultured on MWCNTs grown on a silicon substrate, the response to this nanotubular surface was demonstrated as high cell viability and exceptional cell adhesion without any functionalization of the nanotubes [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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In vitro evaluation of osteoblast responses to carbon nanotube-coated titanium surfaces

et al. 2016

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BackgroundThe effects of surface roughness and carboxyl functionalization of multi-walled carbon nanotubes (MWCNTs) mixed with collagen coated onto titanium (Ti) substrates on MC3T3-E1 osteoblasts were evaluated.MethodsThe proliferation, differentiation, and matrix mineralization were investigated using (1) smooth-surfaced Ti discs, (2) Ti discs coated with collagen and MWCNT (Ti-MWCNT), and (3) Ti discs coated with collagen and MWCNT-COOH (Ti-MWCNT-COOH) for applications in orthodontic mini screw implants (MSIs). The coatings were uniform when analyzed using scanning electron microscopy (SEM), and surface roughness was evaluated by surface profilometry that demonstrated similar surface roughness (Ra, mean ± SD) in the MWCNT (0.83 ± 0.02 μm) and MWCNT-COOH (0.84 ± 0.01 μm) groups. MTT (3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide) assay was performed after days 1, 3, and 7 to assess proliferation. Alkaline phosphatase (ALP)-specific activity was assessed after day 7 to quantify differentiation. Alizarin red staining was measured after day 28 to quantify matrix mineralization. All data were analyzed with JMP Pro11 software (SAS, USA) with a statistical significance of p < 0.05.ResultsSurface profilometry demonstrated similar surface roughness (Ra, mean ± SD) in the MWCNT (0.83 ± 0.02 μm) and MWCNT-COOH (0.84 ± 0.01 μm) groups. On day 7, ALP assay showed that MWCNT-COOH (mean ± SD 0.98 ± 0.26 U/μg of protein) enhanced cell differentiation when compared to the uncoated group (p = 0.05). Alizarin red staining after 28 days of cell culture revealed that MWCNT-COOH (mean ± SD 1.5 ± 0.2 OD405) increased (p = 0.03) matrix mineralization when compared to the uncoated group (0.9 ± 0.09 OD405).ConclusionsThis study showed that coatings containing MWCNT-COOH (increased hydrophilic surface chemistry) influence osteoblast proliferation, differentiation, and matrix mineralization and should be further studied for applications in orthodontic MSIs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro evaluation of osteoblast responses to carbon nanotube-coated titanium surfaces

et al. 2016

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“…Some previous investigation demonstrated that the anticoagulation of MWCNTs was greatly influenced by surface functional groups. In our previous work, we focused on cell and blood compatibility of MWCNTs by different functional groups [ 16 - 20 ]. The improved anticoagulation was observed in COOH + implantation-MWCNTs when compared with pristine MWCNTs [ 13 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous work, we focused on cell and blood compatibility of MWCNTs by different functional groups [ 16 - 20 ]. The improved anticoagulation was observed in COOH + implantation-MWCNTs when compared with pristine MWCNTs [ 13 , 16 ]. The method of COOH + implantation introduced functional carboxyl groups into the surfaces of materials.…”

Section: Introductionmentioning

confidence: 99%

The enhanced anticoagulation for graphene induced by COOH+ ion implantation

et al. 2015

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Graphene may have attractive properties for some biomedical applications, but its potential adverse biological effects, in particular, possible modulation when it comes in contact with blood, require further investigation. Little is known about the influence of exposure to COOH+-implanted graphene (COOH+/graphene) interacting with red blood cells and platelets. In this paper, COOH+/graphene was prepared by modified Hummers' method and implanted by COOH+ ions. The structure and surface chemical and physical properties of COOH+/graphene were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurement. Systematic evaluation of anticoagulation, including in vitro platelet adhesion assays and hemolytic assays, proved that COOH+/graphene has significant anticoagulation. In addition, at the dose of 5 × 1017 ions/cm2, COOH+/graphene responded best on platelet adhesion, aggregation, and platelet activation.

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Composite Nanocoatings of Biomedical Magnesium Alloy Implants: Advantages, Mechanisms, and Design Strategies

Dai

Xiong

et al. 2023

Advanced Science

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The rapid degradation of magnesium (Mg) alloy implants erodes mechanical performance and interfacial bioactivity, thereby limiting their clinical utility. Surface modification is among the solutions to improve corrosion resistance and bioefficacy of Mg alloys. Novel composite coatings that incorporate nanostructures create new opportunities for their expanded use. Particle size dominance and impermeability may increase corrosion resistance and thereby prolong implant service time. Nanoparticles with specific biological effects may be released into the peri‐implant microenvironment during the degradation of coatings to promote healing. Composite nanocoatings provide nanoscale surfaces to promote cell adhesion and proliferation. Nanoparticles may activate cellular signaling pathways, while those with porous or core–shell structures may carry antibacterial or immunomodulatory drugs. Composite nanocoatings may promote vascular reendothelialization and osteogenesis, attenuate inflammation, and inhibit bacterial growth, thus increasing their applicability in complex clinical microenvironments such as those of atherosclerosis and open fractures. This review combines the physicochemical properties and biological efficiency of Mg‐based alloy biomedical implants to summarize the advantages of composite nanocoatings, analyzes their mechanisms of action, and proposes design and construction strategies, with the purpose of providing a reference for promoting the clinical application of Mg alloy implants and to further the design of nanocoatings.

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DIFFERENCES IN CYTOCOMPATIBILITY BETWEEN MWCNTs AND CARBOXYLIC FUNCTIONALIZED MWCNTs

Cited by 7 publications

References 17 publications

In vitro evaluation of osteoblast responses to carbon nanotube-coated titanium surfaces

In vitro evaluation of osteoblast responses to carbon nanotube-coated titanium surfaces

The enhanced anticoagulation for graphene induced by COOH+ ion implantation

Composite Nanocoatings of Biomedical Magnesium Alloy Implants: Advantages, Mechanisms, and Design Strategies

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