Introduction: The physical fitness of college students incites an alarming concern. Although the importance of fitness tests for college students has improved, the overall level and range are low. This requires colleges and universities to increase sports awareness and enrich their exercise methods. Relevant scientific articles can assist universities in promoting college students' physical and mental health. Objective: Analyze the effects of exercise on college students' physical fitness. Methods: We randomly selected 30 college students with normal cardiopulmonary function as volunteers for the research. Walking for 30 minutes was promoted, followed by a rapid walking test for college students. Factors such as breathing, heart rate, and other indicators during exercise were collected, measured, and evaluated. Results: The students in the research had good correlations with each index of oxygen uptake, CO2 exhalation, ventilation ratio, and heart rate during uniform running. The value of the indices tended to stabilize about 5 minutes after exercise. Conclusion: Active running exercise can improve aerobic endurance in college students. Physical fitness was improved in this trial. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.